1. Denaturation of parental plasmid
2. Annealing of mutagenic primers

3. Amplification by QuikChange using parenetal template
-primers are represented by pppxpp> where x is the point mutation and > indicates elongation direction
-two linear strands of DNA with the mutations incorporated are generated
		┌-----------------------┐
		| ┌---<ppxppp |-------┐	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| └-------------------┘ |
		└-----------------------┘

		┌---| pppxpp>-----------┐
		| ┌-------------------┐	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| └-------------------┘ |
		└-----------------------┘

4. Denaturation 
-linear strands of mutant DNA are separated from the parental DNA

						 pppxpp>------------------------------------------|
	
	|----------------------------------------<ppxppp


5.Annealing of mutagenic primers to mutant DNA and parental template
- Annealing of mutagenic primers to parental template is the same fashion as in (3)
- Annealing of mutagenic primers to mutant DNA is as shown below:

						 pppxpp>------------------------------------------|
						 <ppxppp
	
	|----------------------------------------<ppxppp
						 pppxpp>


6. Elongation
-Mutagenic primers annealing to the parental template will amplify as in (3)
-Mutagenic primers annealing to mutant DNA cannot elongate because there is no nucleotide downstream due to the primer design used in the protocol. In order for a primer to use a PCR product as the template, it has to anneal to the 3' end of the new DNA, not 5' end as in this case.

.
.
.
.
(after many PCR cycles)


7. Hybridisation of mutant PCR product

		┌---| pppxpp>-----------┐
		| ┌---<ppxppp |-------┐	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| |		      |	|
		| └-------------------┘ |
		└-----------------------┘