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	<title>BioForum Method Discussion</title>
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		<pubDate>Tue, 29 Mar 2005 22:32:47 -0800</pubDate>
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		<pubDate>Mon, 28 Mar 2005 19:56:07 -0800</pubDate>
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		<title>¶¨Á¿MSP</title>
		<link>http://www.protocol-online.org/forums/index.php?showtopic=4798</link>
		<description><![CDATA[ÎÒµÄMSP»ù±¾³É¹¦£¬Ö¤Ã÷ÒýÎïÊÇ¿ÉÒÔµÄ£¬ÓÐÒ»µã¾­Ñé¾ÍÊÇÈÈÆô¶¯PCR·Ç³£ÖØÒª£¬ÓÈÆäÔÚ·Ç¼×»ù<br />»¯ÌØÒìÐÔÒýÎïÀ©ÔöÌåÏµÖÐ£¬·ÇÈÈÆô¶¯²»¿É£¡<br />ÏÂÒ»²½´òËã½øÐÐ¶¨Á¿MSP£¬ÏÖÔÚÕý¿àÓÚÑ¡ÔñÌ½ÕëÓÐ¼¸¸öÎÊÌâÇë½Ì£º<br />1. ÒýÎïÉè¼ÆÓëÌ½ÕëºÏ³É£º¾­¹ýMETHPRIMERÉè¼ÆµÄÁ½¶ÔÒýÎïµÄTmÖµ·Ö±ðÎª£º¼×»ù»¯ÌØÒìÐÔ69.54£<br />¬69.57£»·Ç¼×»ù»¯ÌØÒìÐÔ59.88£¬65.94£»ÒýÎïºÏ³Éµ¥ÉÏÓëÈí¼þ¸ø³öµÄTmÖµÏà²î½Ï´ó£¬ÒªµÍ5¶<br />È<br />×óÓÒ£¬¶øÔÚÊµ¼ÊMSP¹ý³ÌÖÐ·Ç¼×»ù»¯ÌØÒìÐÔÒýÎïµÄÍË»ðÎÂ¶ÈÖ»ÓÐ52¶È£¬¶¨Á¿PCRµÄÌ½ÕëÒªÇóÒý<br />Î<br />ïÓÐ½Ï¸ßµÄÍË»ðÎÂ¶È£¬¿ÉÊÇ²»Í¬µÄÌ½ÕëºÏ³É¹«Ë¾¿Ú¾¶ÍêÈ«²»Í¬£¬ÓÐµÄËµ²»ÄÜºÏ³É£¬ÓÐµÄËµÍêÈ<br />«<br />¿ÉÒÔºÏ³É£¬ÎÒÓÐÐ©µ£ÐÄ¡£<br />2.  Ì½ÕëÐòÁÐ·½Ãæ£ºÎÒ¿´µ½µÄ²Î¿¼ÎÄÏ×ÉÏ¼×»ù»¯ÌØÒìÐÔÓë·Ç¼×»ù»¯ÌØÒìÐÔÌåÏµµÄÌ½ÕëÐòÁÐÊÇ²»Í¬<br />µÄ£¬¶øÇÒ»¹°üÀ¨ÁË1£­2¸öCpG¼î»ù¶Ô¡£ÎÒ×Ô¼ºÈÏÎªÒýÎïÉè¼ÆÒÑ¾­ÄÜ¹»Çø·Ö¼×»ù»¯Óë·Ç¼×»ù»¯Ð<br />ò<br />ÁÐ£¬Ì½ÕëÊÇ·ñ¿É²»±ØÔÙ°üÀ¨CpG¼î»ù¶Ô£¿ÕâÑùÁ½¸öÌåÏµ¿É¹²ÓÃÒ»¸öÌ½Õë£¬Ò»·½Ãæ½ÚÊ¡Ò»Ð©£¬Á<br />í<br />Ò»·½ÃæÌ½ÕëÉè¼Æ·½Ãæ¾ÍÃ»ÓÐÄÇÃ´¾ÖÏÞ£ºÎÒµÄÁ½¶ÔÒýÎïÖ®¼äµÄÐòÁÐ¿¿½üÉÏÓÎÒýÎïµÄµØ·½Ö»ÓÐÒ»<br />¸<br />öCpG¼î»ù¶Ô£¬Ì½ÕëÉè¼Æ·¶Î§±È½Ï¾ÖÏÞ¡£]]></description>
		<pubDate>Wed, 12 Jan 2005 17:30:50 -0800</pubDate>
		<guid>http://www.protocol-online.org/forums/index.php?showtopic=4798</guid>
	</item>
	<item>
		<title>Ask for advice on a protein study</title>
		<link>http://www.protocol-online.org/forums/index.php?showtopic=4755</link>
		<description><![CDATA[Hi, everyone <br />I want to study a protein functions. It is found that this protein locates in <br />both nucleus and cytoplasm but mainly aggregate in nucleus. So what kind of <br />functions  I can guess this protein has and what kind of experiments I can <br />try? <br /> <br />Thank you]]></description>
		<pubDate>Sun, 09 Jan 2005 20:01:54 -0800</pubDate>
		<guid>http://www.protocol-online.org/forums/index.php?showtopic=4755</guid>
	</item>
	<item>
		<title>Çë½Ì°æÖ÷</title>
		<link>http://www.protocol-online.org/forums/index.php?showtopic=4744</link>
		<description><![CDATA[ÎÒÊÇÐÂÀ´µÄ£¬ÏÈ±¨¸öµ½°¡£¡£¡£¡<br /><br />ÕâÁ½Ìì¿´ÎÄÏ×£¬·¢ÏÖÓÐÐ©ÎÄÏ×ÔÚÉè¼ÆÒýÎïµÄÊ±ºòÌØ±ðÓÃÀ¨ºÅÀ¨³öÒ»¸ö×î5'¶ËµÄÖ»ÓÐCGµÄ³¤¶Ì²»Ò»µÄÆ¬¶Ï£¬ËµÊÇÊ²Ã´CG Clamp£¬²»ÖªÕâÊÇ¸öÊ²Ã´¶«¶«°¡£¿<br /><br /><br />Õâ¸öÂÛÌ³ÊÇÎÒÒ»Ö±ÔÚÕÒµÄ£¬Ï£ÍûÄÜÐ­Öú°æÖ÷°ÑËü¸ãµÄ¸üÍú£¡£¡£¡]]></description>
		<pubDate>Sat, 08 Jan 2005 05:28:32 -0800</pubDate>
		<guid>http://www.protocol-online.org/forums/index.php?showtopic=4744</guid>
	</item>
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