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BioForum _ Cell Biology _ Human Macrophage cell line - quick help please
Posted by: Fred-33 Sep 8 2004, 03:38 AM
I need to know if a human macrophage cell line exists at time.
Many thanks for help.
Posted by: Fred-33 Sep 8 2004, 06:44 AM
I found my answer on atcc site
here are some useful lines from spleen ant monocytes/macrophages
HB-8902
CRL-9850
CRL-9852
CRL-9853
CRL-9854
CRL-9855
CRL-9856
Posted by: serglom Nov 14 2004, 02:03 PM
here are some useful lines from spleen ant monocytes/macrophages
HB-8902
CRL-9850
CRL-9852
CRL-9853
CRL-9854
CRL-9855
CRL-9856
you can also use THP-1 monocyte cell line and turn them into macrophages with TPA or PMA
Posted by: emilydeanna Jan 28 2009, 02:01 PM
I have just started culturing THP-1 cells.
After sub-culturing, I wanted to freeze down some of these cells. I froze a vial of cells with my normal freezing media (2x, 20% DMSO and 80% FBS). When I thawed the cells they were viable. After 48h the cells had switched from being in suspension to becoming adherent.
Is this from the DMSO?
Should I use glycerol to cryopreserve instead?
Posted by: SuMi Feb 3 2009, 06:58 AM
After sub-culturing, I wanted to freeze down some of these cells. I froze a vial of cells with my normal freezing media (2x, 20% DMSO and 80% FBS). When I thawed the cells they were viable. After 48h the cells had switched from being in suspension to becoming adherent.
Is this from the DMSO?
Should I use glycerol to cryopreserve instead?
Yes I have found that DMSO causes my cells to differentiate. 20% DMSO is very high - 10% is enough. I now use a special freezing medium for my cells as i had a huge problem with differentiation. When you thaw your cells you should spin them down at a low speed and remove the freezing medium to remove the DMSO.
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