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BioForum _ Immunology _ Macrophage culture

Posted by: Sinead Jun 8 2002, 05:41 AM

I am trying to culture macrophages from whole blood. What I am looking for is a way to assess the purity of my macrophage population adhered to the culture dish.

Posted by: RichardColes Jun 27 2002, 05:56 PM

Use Mac-1 and GR-1(granulocytes) antibodies and do FACS analysis or something like that.

Posted by: febstein Nov 24 2003, 03:43 AM

Alternatively, have you ever tried to generate macrophages from monocytes in the presence of GM-CSF ?

Posted by: Victor Dec 19 2003, 02:45 PM

Can also try diI labeled acetylated LDL to test their phagocytic activity.

Posted by: rakeshbhu Nov 29 2004, 07:24 AM

Time of adheration and washing of adhered monocytes are very important. Standardized it.