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BioForum _ Microbiology _ Cycloheximide Concentration

Posted by: mattbat7 Oct 7 2009, 07:23 AM

Hi,

I'm trying to inhibit a stock strain of wild-type Saccharomyces with cycloheximide.....but am unsure what concentration to use.


Does anybody know of a resource i can use, or what concentration is commonly used?

Thanks

Mattbat

Posted by: GeorgeWolff Oct 7 2009, 01:38 PM

Matt - the levels I've used are ~ 0.5g/liter.

Posted by: GeorgeWolff Oct 7 2009, 03:31 PM

Saw (old) paper (http://jb.asm.org/cgi/content/abstract/86/5/956 ) reporting apparent cycloheximide induction of petite variant of related species.

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