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Cell Biology

Questions on cell and tissue culture, cytometry ...

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MTT, Proliferation and Cytotoxicity Assay

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Topic	Stats	Last Post Info
Thawed cells spent 4 hours extra in DMSO.. problem? Started by thebromar, 30 Oct 2012 culture, dmso, thawed, frozen and 1 more...	1 reply 298 views	John Forsberg 30 Oct 2012
conditioned medium for ischaemia study Started by chastings, 29 Nov 2012 dialysis, hypoxia	1 reply 294 views	bob1 29 Nov 2012
Cloning repetitive sequences in mammalian cells Started by Chelo, 07 Apr 2013	3 replies 293 views	bob1 08 Apr 2013
Commercial source Stomach Smooth Muscle Cell lysate Started by saikat, 12 Oct 2012 stomach, smooth muscle cells and 2 more...	1 reply 291 views	Inmost sun 30 Oct 2012
Can I activate signaling pathways without extracellular stimuli? Started by MariaGreen, 16 Feb 2013 signaling pathways, MAPK and 1 more...	4 replies 290 views	MariaGreen 17 Feb 2013
Western blot and immunofluorescence staining Started by emou, 20 Feb 2013	9 replies 288 views	emou 24 Feb 2013
HELP-zebrafish antibody staining after day3 Started by luc, 28 Oct 2012	6 replies 284 views	luc 29 Oct 2012
Cell Therapies Started by jason77, 10 Sep 2012	2 replies 282 views	bob1 11 Sep 2012
Cells detach during bodipy 493/503 staining Started by julnobody, 28 Sep 2012	1 reply 281 views	bob1 28 Sep 2012
HEK293T and G418 Started by science noob, 18 Sep 2012	1 reply 280 views	cellthetruth 18 Sep 2012
Carbon release from human tissue? Started by BobMilan, 04 Apr 2013	3 replies 273 views	arnoldous 14 May 2013
PBS (-) preparation Started by selma100, 01 Oct 2012	1 reply 272 views	Ameya P 12 Oct 2012
DCF-DA assay for ROS measurement with 3T3 cells Started by lucadandy, 10 Jan 2013 dcf-da, ros, diclorofluorescein and 2 more...	0 replies 269 views	lucadandy 10 Jan 2013
System to label DNA Started by lyok, 09 Feb 2013	6 replies 268 views	lyok 11 Feb 2013
Strange Cell Morphology & Cell Debris Following Lentiviral Transduction Started by chabraha, 23 Oct 2012 lentivirus, thp-1, transduction and 1 more...	2 replies 267 views	chabraha 23 Oct 2012
Presence of melanin in melanin containing cell line Started by uschaka, 15 Nov 2012 MeWo, melanin	1 reply 265 views	bob1 15 Nov 2012
Primary culture of breast tumor cells Started by waldomedina, 25 Oct 2012 cell culture, breast tumor cells	1 reply 258 views	Curtis 25 Oct 2012
Determine cell proliferation by viability assay ? Started by Tabaluga, 30 Jan 2013 Cell Titer Glo, proliferation	6 replies 257 views	Tabaluga 01 Feb 2013
Alizarin Red Staining on scaffolds... Started by cshmech, 10 Jan 2013	1 reply 256 views	cshmech 14 Jan 2013
cytotoxicity on HEK 293 Started by cell 293, 28 Mar 2013 cytotoxicity	0 replies 252 views	cell 293 28 Mar 2013
Isolation of BOTH cells AND serums from mouse blood Started by wernerlael, 16 Oct 2012 isolation. mouse. cells.	5 replies 250 views	zodiac1505 18 Oct 2012
Please help me take a look at my cells to see whether is it contaminated Started by CYJ, 22 Mar 2013	3 replies 250 views	rhombus 26 Mar 2013
Calculating enzyme activity Started by PaulF, 20 Nov 2012	2 replies 249 views	PaulF 21 Nov 2012
Accessing Transfection efficiency using fluorescence microscope (Alexa Fluor 555 Started by Neanderthal, 19 Oct 2012 fluorescence microscope and 2 more...	1 reply 248 views	bob1 19 Oct 2012
Fast/easy way to measure cell size Started by Vassil, 27 Feb 2013	4 replies 247 views	Vassil 18 Mar 2013