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Topic	Stats	Last Post Info
Pinned Cryopreservation (freezing cells) without using Freezing Container Started by flashboy, 12 Feb 2002 1 2	Hot 15 replies 20,604 views	citomebo 15 Feb 2012
Pinned Freezing and thawing of cells Started by shilpi13, 09 Sep 2004 1 2 3	Hot 32 replies 36,103 views	unknownko 07 Jan 2012
Pinned virus vector for transfection Started by picomagalhaes, 03 Mar 2005	10 replies 15,611 views	CloneMan 15 Sep 2011
Pinned help - stable transfection clone pick up Started by geness, 08 Apr 2004 1 2	Hot 17 replies 22,563 views	frna11 14 Aug 2011
Pinned Problem in culturing MCF-7 cell line! Started by wuahaha, 28 Feb 2005 1 2	Hot 20 replies 23,500 views	ralfstevens 09 Aug 2011
Pinned Population doubling time of keratinocyte,etc. Started by sycay, 01 Mar 2005	Hot 14 replies 18,546 views	shane 21 Jun 2010
Co-localisation Analysis - immunofluorescence Started by Natalia KM, 15 Nov 2018	3 replies 308 views	bob1 26 Nov 2018
black spots are found in cells and medium Started by hzaubl, 31 Oct 2018	1 reply 262 views	bob1 01 Nov 2018
remove GH from FBS Started by wuxx0153, 04 Oct 2018 to do or not to do	0 replies 348 views	wuxx0153 04 Oct 2018
Cell Membranes as an active barrier Started by dga, 23 Sep 2018	1 reply 289 views	Inbox 02 Oct 2018
Normalise cell count for protein analysis Started by Chack, 14 Aug 2018	3 replies 308 views	bob1 15 Aug 2018
Induction of oxidative stress Started by gsb1989, 14 Aug 2018 oxidative stress and 4 more...	0 replies 367 views	gsb1989 14 Aug 2018
Blockage of cadherin bindings of MCF-10A cells without harming hydrogels and cal Started by ecisue, 01 Aug 2018 EDTA, MCF10A, epithelial cell and 2 more...	2 replies 767 views	ecisue 01 Aug 2018
1st researcher linking EVs to viruses Started by JustWondering..., 19 Jul 2018 vesicle, virus, research and 1 more...	0 replies 338 views	JustWondering... 19 Jul 2018
Should Lineage marker cocktail be used in cell lines to remove lineage specific Started by rv1234, 18 Apr 2018 Cell Culture, FACS, CellLines and 1 more...	1 reply 803 views	Creative Biolabs 19 Jun 2018
Does anyone have these (sadly-discontinued) anti-BrdU antibodies in their freeze Started by cmerrick, 25 Apr 2018 immunofluorescence and 4 more...	0 replies 631 views	cmerrick 25 Apr 2018
When overexpressing several genes using different vectors, what negative control Started by Lizzyf23, 30 Oct 2017 over expression, negative control and 3 more...	2 replies 1,490 views	Lizzyf23 31 Oct 2017
NB-4 cells Started by Bennie George , 24 Oct 2017 NB-4 cells	0 replies 960 views	Bennie George 24 Oct 2017
about measuring luciferase activity in living cells Started by stormlina, 10 Sep 2009	9 replies 4,093 views	Misha_Koksharov 29 Sep 2017
crystal violet staining Started by fabipp, 14 Aug 2012 staining	6 replies 2,883 views	Zagami Francesco 08 Sep 2017
Transfection Started by Puppy Singh, 24 Jul 2017	2 replies 1,039 views	Puppy Singh 26 Jul 2017
What is the best dopaminergic cell line for a in-vitro parkinson's disease m Started by Katlnz, 12 Jul 2017 parkinsons, neuronal cell line and 3 more...	0 replies 1,592 views	Katlnz 12 Jul 2017
Freezing cell lysate for later Luciferase Assay Started by matheusmlot, 18 Jan 2017 transfection, luciferase assay and 2 more...	0 replies 2,090 views	matheusmlot 18 Jan 2017
Lysis of osteoblastic cell lines for dual luciferase assay Started by ocram, 31 Oct 2016	0 replies 1,246 views	ocram 31 Oct 2016
Fusion protein instability at ER membrane Started by miST32, 07 Sep 2016 ER membrane, Dimerization, FKBP and 2 more...	0 replies 1,354 views	miST32 07 Sep 2016