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Topic	Stats	Last Post Info
Pinned Cryopreservation (freezing cells) without using Freezing Container Started by flashboy, 12 Feb 2002 1 2	Hot 15 replies 19,693 views	citomebo 15 Feb 2012
Pinned Freezing and thawing of cells Started by shilpi13, 09 Sep 2004 1 2 3	Hot 32 replies 34,999 views	unknownko 07 Jan 2012
Pinned virus vector for transfection Started by picomagalhaes, 03 Mar 2005	10 replies 14,832 views	CloneMan 15 Sep 2011
Pinned help - stable transfection clone pick up Started by geness, 08 Apr 2004 1 2	Hot 17 replies 21,611 views	frna11 14 Aug 2011
Pinned Problem in culturing MCF-7 cell line! Started by wuahaha, 28 Feb 2005 1 2	Hot 20 replies 22,554 views	ralfstevens 09 Aug 2011
Pinned Population doubling time of keratinocyte,etc. Started by sycay, 01 Mar 2005	Hot 14 replies 17,748 views	shane 21 Jun 2010
Does anyone have these (sadly-discontinued) anti-BrdU antibodies in their freeze Started by cmerrick, 25 Apr 2018 immunofluorescence and 4 more...	0 replies 389 views	cmerrick 25 Apr 2018
Should Lineage marker cocktail be used in cell lines to remove lineage specific Started by rv1234, 18 Apr 2018 Cell Culture, FACS, CellLines and 1 more...	0 replies 306 views	rv1234 18 Apr 2018
When overexpressing several genes using different vectors, what negative control Started by Lizzyf23, 30 Oct 2017 over expression, negative control and 3 more...	2 replies 1,261 views	Lizzyf23 31 Oct 2017
NB-4 cells Started by Bennie George , 24 Oct 2017 NB-4 cells	0 replies 582 views	Bennie George 24 Oct 2017
about measuring luciferase activity in living cells Started by stormlina, 10 Sep 2009	9 replies 3,900 views	Misha_Koksharov 29 Sep 2017
crystal violet staining Started by fabipp, 14 Aug 2012 staining	6 replies 2,587 views	Zagami Francesco 08 Sep 2017
Transfection Started by Puppy Singh, 24 Jul 2017	2 replies 800 views	Puppy Singh 26 Jul 2017
What is the best dopaminergic cell line for a in-vitro parkinson's disease m Started by Katlnz, 12 Jul 2017 parkinsons, neuronal cell line and 3 more...	0 replies 1,394 views	Katlnz 12 Jul 2017
Freezing cell lysate for later Luciferase Assay Started by matheusmlot, 18 Jan 2017 transfection, luciferase assay and 2 more...	0 replies 1,862 views	matheusmlot 18 Jan 2017
Lysis of osteoblastic cell lines for dual luciferase assay Started by ocram, 31 Oct 2016	0 replies 1,083 views	ocram 31 Oct 2016
Fusion protein instability at ER membrane Started by miST32, 07 Sep 2016 ER membrane, Dimerization, FKBP and 2 more...	0 replies 1,118 views	miST32 07 Sep 2016
The true monomers making RNA and DNA ? Started by Shouhei Blaser, 01 Sep 2016	1 reply 797 views	bob1 01 Sep 2016
Co-transfection of multiple plasmids Started by DrSnood, 29 Jul 2016 transfection	1 reply 1,220 views	827753 02 Aug 2016
Culturing LoVo cells Started by MelShizue, 28 Jun 2016	1 reply 958 views	bob1 06 Jul 2016
The adipose cell culturing Started by 2444256703, 29 Oct 2015 adipose cell	2 replies 1,609 views	JJoanna 28 Jun 2016
Luciferase assay - experimental design Started by DrSnood, 17 May 2016 luciferase reporter	1 reply 1,021 views	bob1 20 May 2016
Synchronization of HEK293T cells for IP Started by ghernandez, 02 Feb 2016 IP, cell cycle, HEK293T	2 replies 1,915 views	Andrea Fortina 16 May 2016
Cytotoxicity of hoechst staining in overnight experiment Started by Badette, 18 Mar 2016 Cell Biology, Hoechst and 2 more...	1 reply 1,213 views	Andrea Fortina 16 May 2016
Cell detachment in clonogenic assay Started by JDSBlueDevl, 13 May 2016	0 replies 918 views	JDSBlueDevl 13 May 2016