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ChIP and Next Generation Sequencing

Please also visit archived discussions on the topic of ChIP (1999-2009) and ChIP (2009-Present), sonication issues (sonication 1 and sonication 2) and ChIP-Seq in the ChIP Forum

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Topic	Stats	Last Post Info
Pinned What do people use as sequencing control in ChIP-Seq Started by TracyDuke, 28 Jul 2009	11 replies 13,266 views	Zohreh_fa 03 Mar 2016
Pinned Purify ChIP DNA using Qiagen spin filter Started by epigenetics, 30 Nov 2009	8 replies 6,680 views	dnafactory 16 Jul 2015
Pinned % INPUT - ChIP Started by dna_nerd, 05 Feb 2009 1 2	Hot 20 replies 26,979 views	leuyen 23 Jun 2015
Pinned Shearing (sonication) problem for ChIP Started by timjim, 10 Jun 2009 sonication, shearing, chip 1 2	Hot 28 replies 17,606 views	rajanagre 01 Aug 2013
Pinned How to analyze ChIP PCR data Started by krystle, 13 Feb 2011 ChIP, data analysis, result and 1 more...	10 replies 8,024 views	pcrman 11 Jul 2013
Pinned Sonication Vs MNase Started by Dukey, 14 Apr 2011 1 2	Hot 20 replies 7,091 views	nanook 09 Sep 2011
Pinned chromatin shearing using Bioruptor sonicator Started by thammiey, 14 Feb 2011	7 replies 5,300 views	Wonyong 23 Aug 2011
Pinned help regarding ChIP assay after sonication with bioruptor Started by varma_ran, 07 Dec 2010	9 replies 4,896 views	hamidk 23 Mar 2011
Pinned Samples for ChIP-seq library preparation Started by doudou30, 22 Apr 2010 1 2	Hot 15 replies 9,165 views	doudou30 22 Sep 2010
Pinned Is my antibody ChIP grade? Started by nigelcr, 10 Mar 2010	10 replies 7,478 views	nc03 27 Apr 2010
Pinned ChIP Foaming and other woes (archived) Started by bioforum, 31 Jan 2009	4 replies 8,035 views	JPchip 01 May 2009
Pinned ChIP - Foaming in sonication and other woes Started by badcell, 27 Jan 2005	12 replies 10,316 views	Ming 29 Mar 2005
How to identify the genomic target region for positive control in ChIP assay Started by Sandeep Gupta, 17 Aug 2016 ChiP	0 replies 170 views	Sandeep Gupta 17 Aug 2016
Miseq Minimum number of RNAseq reads needed for comparison of bacterial stress v Started by Chris22, 26 May 2016 RNAseq, Single bacteria, In-vitro and 2 more...	2 replies 386 views	Chris22 14 Jul 2016
Bioanalyzer for ChIPed DNA-need help! Started by Zohreh_fa, 13 Jun 2016 chip, bioanalyzer, chip-seq	0 replies 337 views	Zohreh_fa 13 Jun 2016
Time for Agilent Nextselect to come Started by biofingers, 29 May 2016 nextselect and 1 more...	3 replies 447 views	Trof 02 Jun 2016
Acceptable Fold enrichment threshold fro ChIP? Started by Zohreh_fa, 25 May 2016	0 replies 308 views	Zohreh_fa 25 May 2016
Sonication woes for Chip-seq, using Bioruptor Started by jancho.wang, 16 Jun 2012 sonication, bioruptor, chip-seq	7 replies 1,968 views	AtomWang 29 Mar 2016
fragmentation single cells Started by molbio1234, 18 Jan 2016 fragmentation	0 replies 451 views	molbio1234 18 Jan 2016
Answered How many NGS readings for a virus genome? Started by Curtis, 15 Nov 2015	6 replies 600 views	Trof 21 Nov 2015
Illumina MiSeq Started by vanu, 14 Oct 2015 OTUs, QIIME Microbiota	0 replies 1,065 views	vanu 14 Oct 2015
CHIP-seq problem with yeast gDNA!!! Started by lorenzo, 31 Aug 2015 chip-seq	0 replies 673 views	lorenzo 31 Aug 2015
Chip Assay-Cell type? Started by Zohreh_fa, 27 Jul 2015	0 replies 588 views	Zohreh_fa 27 Jul 2015
Quantification of input after sonication Started by Gnap, 19 Jul 2015 Chip-seq	0 replies 663 views	Gnap 19 Jul 2015
Negative control for ChIP experiment: Knockout cells Vs IgG Started by dnafactory, 16 Jul 2015	0 replies 566 views	dnafactory 16 Jul 2015