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ChIP and Next Generation Sequencing

Please also visit archived discussions on the topic of ChIP (1999-2009) and ChIP (2009-Present), sonication issues (sonication 1 and sonication 2) and ChIP-Seq in the ChIP Forum

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Topic	Stats	Last Post Info
Pinned What do people use as sequencing control in ChIP-Seq Started by TracyDuke, 28 Jul 2009	11 replies 17,666 views	Zohreh_fa 03 Mar 2016
Pinned Purify ChIP DNA using Qiagen spin filter Started by epigenetics, 30 Nov 2009	8 replies 10,991 views	dnafactory 16 Jul 2015
Pinned % INPUT - ChIP Started by dna_nerd, 05 Feb 2009 1 2	Hot 20 replies 32,795 views	leuyen 23 Jun 2015
Pinned Shearing (sonication) problem for ChIP Started by timjim, 10 Jun 2009 sonication, shearing, chip 1 2	Hot 28 replies 22,937 views	rajanagre 01 Aug 2013
Pinned How to analyze ChIP PCR data Started by krystle, 13 Feb 2011 ChIP, data analysis, result and 1 more...	10 replies 13,931 views	pcrman 11 Jul 2013
Pinned Sonication Vs MNase Started by Dukey, 14 Apr 2011 1 2	Hot 20 replies 11,927 views	nanook 09 Sep 2011
Pinned chromatin shearing using Bioruptor sonicator Started by thammiey, 14 Feb 2011	7 replies 9,480 views	Wonyong 23 Aug 2011
Pinned help regarding ChIP assay after sonication with bioruptor Started by varma_ran, 07 Dec 2010	9 replies 9,003 views	hamidk 23 Mar 2011
Pinned Samples for ChIP-seq library preparation Started by doudou30, 22 Apr 2010 1 2	Hot 15 replies 14,078 views	doudou30 22 Sep 2010
Pinned Is my antibody ChIP grade? Started by nigelcr, 10 Mar 2010	10 replies 11,535 views	nc03 27 Apr 2010
Pinned ChIP Foaming and other woes (archived) Started by bioforum, 31 Jan 2009	4 replies 12,063 views	JPchip 01 May 2009
Pinned ChIP - Foaming in sonication and other woes Started by badcell, 27 Jan 2005	12 replies 11,376 views	Ming 29 Mar 2005
Need some troubleshooting about ChIP - Is it a problem with the antibody? Started by Florian_LB, 14 Dec 2018 ChIPAntibody, Coregulator and 1 more...	2 replies 884 views	Florian_LB 18 Dec 2018
Variable ChiP-qPCR even in the same lysate aliquots Started by Sandeep Gupta, 15 Sep 2017 ChiP, ChiP qPCR	0 replies 1,731 views	Sandeep Gupta 15 Sep 2017
how to do % input calculations from Ct values Started by Sandeep Gupta, 19 Jul 2017 ChiP, qPCR	0 replies 1,945 views	Sandeep Gupta 19 Jul 2017
Radiactive PCR for ChIP quantification¡¡?? Started by autophagator, 28 Sep 2016 ChIP radiactive PCR qPCR	0 replies 1,789 views	autophagator 28 Sep 2016
How to identify the genomic target region for positive control in ChIP assay Started by Sandeep Gupta, 17 Aug 2016 ChiP	0 replies 1,602 views	Sandeep Gupta 17 Aug 2016
Miseq Minimum number of RNAseq reads needed for comparison of bacterial stress v Started by Chris22, 26 May 2016 RNAseq, Single bacteria, In-vitro and 2 more...	2 replies 1,866 views	Chris22 14 Jul 2016
Bioanalyzer for ChIPed DNA-need help! Started by Zohreh_fa, 13 Jun 2016 chip, bioanalyzer, chip-seq	0 replies 1,792 views	Zohreh_fa 13 Jun 2016
Time for Agilent Nextselect to come Started by biofingers, 29 May 2016 nextselect and 1 more...	3 replies 3,265 views	Trof 02 Jun 2016
Acceptable Fold enrichment threshold fro ChIP? Started by Zohreh_fa, 25 May 2016	0 replies 1,600 views	Zohreh_fa 25 May 2016
Sonication woes for Chip-seq, using Bioruptor Started by jancho.wang, 16 Jun 2012 sonication, bioruptor, chip-seq	7 replies 3,172 views	AtomWang 29 Mar 2016
fragmentation single cells Started by molbio1234, 18 Jan 2016 fragmentation	0 replies 1,719 views	molbio1234 18 Jan 2016
Answered How many NGS readings for a virus genome? Started by Curtis, 15 Nov 2015	6 replies 2,079 views	Trof 21 Nov 2015
Illumina MiSeq Started by vanu, 14 Oct 2015 OTUs, QIIME Microbiota	0 replies 3,614 views	vanu 14 Oct 2015