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ChIP and Next Generation Sequencing

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Topic	Stats	Last Post Info
Pinned Purify ChIP DNA using Qiagen spin filter Started by epigenetics, 30 Nov 2009	6 replies 2,531 views	kw917 20 Feb 2013
Pinned Shearing (sonication) problem for ChIP Started by timjim, 10 Jun 2009 sonication, shearing, chip 1 2	Hot 20 replies 12,446 views	chabraha 20 Dec 2012
Pinned What do people use as sequencing control in ChIP-Seq Started by TracyDuke, 28 Jul 2009	9 replies 9,347 views	amdens 22 Dec 2011
Pinned Sonication Vs MNase Started by Dukey, 14 Apr 2011 1 2	Hot 20 replies 3,264 views	nanook 09 Sep 2011
Pinned chromatin shearing using Bioruptor sonicator Started by thammiey, 14 Feb 2011	7 replies 2,309 views	Wonyong 23 Aug 2011
Pinned How to analyze ChIP assay data Started by krystle, 13 Feb 2011	9 replies 2,980 views	chabraha 31 Mar 2011
Pinned help regarding ChIP assay after sonication with bioruptor Started by varma_ran, 07 Dec 2010	9 replies 2,097 views	hamidk 23 Mar 2011
Pinned Samples for ChIP-seq library preparation Started by doudou30, 22 Apr 2010 1 2	Hot 15 replies 5,287 views	doudou30 22 Sep 2010
Pinned % INPUT - ChIP Started by dna_nerd, 05 Feb 2009 1 2	Hot 19 replies 19,584 views	Shan 10 Sep 2010
Pinned Is my antibody ChIP grade? Started by nigelcr, 10 Mar 2010	10 replies 4,358 views	nc03 27 Apr 2010
Pinned ChIP Foaming and other woes (archived) Started by bioforum, 31 Jan 2009	4 replies 5,223 views	JPchip 01 May 2009
Pinned ChIP - Foaming in sonication and other woes Started by badcell, 27 Jan 2005	12 replies 8,580 views	Ming 29 Mar 2005
Can local chromatin structure affect sonication? Started by Evan Y, 11 Apr 2013	3 replies 170 views	Vassil 30 Apr 2013
ChIP for a transcriptional coactivator Started by yds2, 19 Apr 2013	1 reply 170 views	Vassil 30 Apr 2013
Sonication Help! Started by j47027, 19 Apr 2013 Sonication, RNaseA, Proteinase K and 1 more...	3 replies 164 views	Vassil 30 Apr 2013
dscDNA synthesis suitable for Illumina Sequencing Started by sierraocean, 04 Apr 2013 dscDNA, Illumina	0 replies 161 views	sierraocean 04 Apr 2013
ds cDNA synthesis problem Started by doudou30, 19 Oct 2010	1 reply 443 views	sierraocean 04 Apr 2013
Eluting from magnetic beads in ChIP. Started by Trust, 05 May 2010	11 replies 3,048 views	Steph Kyle 02 Apr 2013
Sonicator Optimization Trouble Started by BFS, 23 Mar 2011	4 replies 1,185 views	Vassil 27 Mar 2013
Trouble validating in multiple cell types Started by nleboarder, 25 Mar 2013 H3, H3K27me3, H3K4me3, RPL30 and 1 more...	1 reply 149 views	jerryshelly1 27 Mar 2013
Emergency~~~Storage sonicated ChIP lysate at -80 without centifugation? Started by ketty113, 20 May 2011	2 replies 773 views	Vassil 27 Mar 2013
Negative Control for ChIP realtime PCR in Mouse Started by jiro_killua, 05 Feb 2009	12 replies 5,690 views	nleboarder 25 Mar 2013
histone chip-seq profiles look similar to input! Started by vic17, 25 Feb 2013 histone-chip, chip-seq	4 replies 244 views	vic17 27 Feb 2013
Why there are blobs in my sheared DNA bands? Started by ChIP Kick, 07 Jan 2013 Sheared DNA	0 replies 211 views	ChIP Kick 07 Jan 2013
Used all ChIP lysis buffer Started by ChIP Kick, 04 Jan 2013	0 replies 270 views	ChIP Kick 04 Jan 2013