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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Phospho-Elk1 specific Antibody...which band?? Started by hanze, 18 Feb 2005	5 replies 2,858 views	mdfenko 20 May 2010
Co-immunoprecipitation Started by Starlight83, 29 Oct 2009	10 replies 2,842 views	Starlight83 09 Nov 2009
starry sky western blot... lots of spots. Started by MaggieRoara, 09 Mar 2009	5 replies 2,830 views	MDavies 13 Oct 2009
Subcellular fractionation Started by gradgrunt, 20 Jul 2004	6 replies 2,807 views	$Subcellular fractionation - last post by bob1$ bob1 10 Feb 2009
help! Truncated protein cannot expressed in mammalian cells? Started by butterfly, 09 Mar 2009	6 replies 2,794 views	drckitty 16 Nov 2012
Protein synthesis inhibition Started by lukceg1983, 13 Jul 2009	4 replies 2,791 views	lukceg1983 16 Jul 2009
GST pull down assay Started by MaggieRoara, 19 Mar 2009	4 replies 2,785 views	MaggieRoara 24 Mar 2009
Immunoprecipitation - Bands in negative control Started by protein2, 08 Aug 2009	2 replies 2,759 views	laurequillo 25 Sep 2009
Cleaved GST fusion protein retained on beads! Started by mert1518, 03 Mar 2005	8 replies 2,755 views	cdc2kinase 26 Sep 2012
Immunoprecipitation vs conventional WB Started by Doda, 01 Feb 2009	7 replies 2,753 views	Doda 08 Feb 2009
Bubble test Started by samita, 19 Feb 2009	7 replies 2,722 views	Penguin 04 Mar 2009
band spread in western Started by hjdeal, 07 Mar 2005	9 replies 2,716 views	mabusheh 15 Mar 2005
Gel shift (emsa) with 35S label? Started by Ambrósio, 27 Jul 2009	5 replies 2,703 views	Ambrósio 03 Aug 2009
Semidry Transfer Started by jiro_killua, 19 Feb 2009	4 replies 2,694 views	jiro_killua 20 Feb 2009
software for densitometric analysis Started by charlie*, 04 Aug 2004	1 reply 2,649 views	labrat 04 Aug 2004
Why most commercial protease inhibitor cocktails do not contain EDTA? Started by eilosei, 11 Aug 2009	7 replies 2,627 views	bob1 13 Sep 2010
EMSA and supershift Started by Stef, 18 May 2009	6 replies 2,626 views	Stef 20 May 2009
protein precipitates after dialysis Started by rtandon, 16 Jan 2002	4 replies 2,617 views	labslave 21 Nov 2002
Getting rid of background protein binding on GST beads Started by MaggieRoara, 22 Mar 2009	5 replies 2,607 views	T C 24 Mar 2009
Concentrating Protein Started by tatzilo, 02 Nov 2009	8 replies 2,586 views	dnatrash 19 Feb 2011
protein concentration method Started by yja97, 10 Mar 2009	4 replies 2,573 views	swanny 12 Mar 2009
stacking gel Started by SF_HK, 10 Aug 2009	4 replies 2,547 views	T C 14 Aug 2009
removal of SDS from protein Started by anasyida69, 08 Dec 2009	4 replies 2,540 views	anasyida69 09 Dec 2009
Coomassie blue before protein transfer Started by britkid79, 26 Oct 2009	9 replies 2,528 views	laurequillo 27 Oct 2009
PVDF blot storage Started by cancergeek, 16 Jul 2009	3 replies 2,525 views	$PVDF blot storage - last post by polyfractal$ polyfractal 22 Jul 2009