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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Big differences between BCA and Bradford? Started by vojera, 10 Feb 2010	8 replies 1,742 views	mdfenko 12 Feb 2010
Preparing SDS Sample for Soluble/Insoluble Fractions Started by Sharky, 16 Mar 2010	8 replies 2,365 views	pDNA 23 Jun 2010
Southern blot troubleshoot Started by zodiac1505, 23 Jun 2010	8 replies 1,347 views	bob1 08 Jul 2010
Lactobacillus crude protein extraction? Started by Chris22, 13 Apr 2011	8 replies 969 views	proteaMatt 25 Apr 2011
Dry transfer Started by baboon, 21 Apr 2011	8 replies 1,610 views	knuf 29 Apr 2011
lysis buffer not lysing adherent cells? Started by philman, 08 Feb 2012	8 replies 893 views	philman 08 Feb 2012
problem with protein identification Started by mimosa, 28 May 2012	8 replies 633 views	mimosa 03 Jun 2012
SDS-PAGE sample streaking issue Started by littledamn, 04 Apr 2013	8 replies 517 views	littledamn 13 Apr 2013
problem with spectrophotometer Started by BURCU:), 09 Sep 2004	7 replies 1,861 views	jadefalcon 13 Jan 2005
Polymerization SDS Gel Started by Trinity, 21 Jan 2005	7 replies 3,104 views	mabusheh 09 Mar 2005
Good buffer needed! Started by Joana, 17 Feb 2005	7 replies 2,323 views	leekaming 26 Mar 2005
Ni-NTA problem Started by yongyk, 17 Mar 2005	7 replies 2,196 views	leekaming 28 Mar 2005
Immunoprecipitation vs conventional WB Started by Doda, 01 Feb 2009	7 replies 2,754 views	Doda 08 Feb 2009
Immunoprecipitation (optimization?) problem Started by lab rat, 03 Feb 2009	7 replies 2,958 views	lab rat 22 Feb 2009
Bubble test Started by samita, 19 Feb 2009	7 replies 2,725 views	Penguin 04 Mar 2009
Difference between Nonidet-P40 and Tween20 and TritonX100 Started by MaggieRoara, 19 Mar 2009	7 replies 4,538 views	bob1 03 Sep 2011
questions about cell lysis Started by zx0819, 02 May 2009	7 replies 3,045 views	sudhakar mutyala 07 Jun 2009
Why most commercial protease inhibitor cocktails do not contain EDTA? Started by eilosei, 11 Aug 2009	7 replies 2,633 views	bob1 13 Sep 2010
Loading sample in FPLC loops Started by celvas, 18 Aug 2009	7 replies 3,399 views	dxm 25 Aug 2009
LC-MS protocol Started by my3, 29 Oct 2009	7 replies 2,089 views	Prep! 29 Oct 2009
storing WB blots in PBS-tween Started by fred197237, 12 Nov 2009	7 replies 2,185 views	fred197237 13 Nov 2009
immunoprecipitation and preclearing incubation times Started by cellgene, 23 Apr 2010	7 replies 1,578 views	Mardahl 05 Sep 2012
antibody purification - ammonium sulfate precipitation Started by chicho, 28 Jun 2010	7 replies 1,260 views	Prep! 01 Jul 2010
ELISA peptide coating problem =( Started by edilberto ojeda, 14 Jul 2010	7 replies 1,220 views	edilberto ojeda 12 Aug 2010
making gel in advance Started by SF_HK, 06 Nov 2010	7 replies 961 views	mdfenko 22 Nov 2010