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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
protein-protein interaction Started by zogene, 20 Jan 2013	4 replies 298 views	zogene 30 Jan 2013
How should I use MG132? Started by WYpiao, 28 Jan 2013 MG132	2 replies 219 views	WYpiao 29 Jan 2013
Protein concentration of a labelled membrane protein Started by Vinnie, 27 Jan 2013	2 replies 262 views	Vinnie 28 Jan 2013
isoelctric gels hard to stain? Started by joy123, 22 Jan 2013	2 replies 188 views	joy123 26 Jan 2013
Protein purification method to isolate elicitor with biological activity Started by RameshSundar, 23 Jan 2013 Purified protein and 1 more...	1 reply 207 views	mdfenko 23 Jan 2013
Adh1 Started by An-nur Al-Irsyad, 22 Jan 2013 Where is Adh1 secreted?	0 replies 184 views	An-nur Al-Irsyad 22 Jan 2013
protein Protein Linking Started by Gunalan, 17 Dec 2012 protein bindings	5 replies 391 views	bob1 20 Jan 2013
purification and binding of protein in denaturing conditions Started by shivu11, 18 Jan 2013 poor solubility and 3 more...	2 replies 209 views	Adrian K 20 Jan 2013
protein sample floating out of the tricine gel Started by catalina_roe, 15 Jan 2013 tricine-SDS-PAGE, protein sample	3 replies 267 views	Rosewater 18 Jan 2013
General chat about tween-20 as a WB block and wash Started by Joe101, 18 Jan 2013 western blot, tween 20, wash and 2 more...	1 reply 251 views	bob1 18 Jan 2013
Concentration of secreted bacterial proteins using DOC-TCA precipitation Started by spiv15, 18 Jan 2013 secreted, TCA, precipitation and 2 more...	0 replies 247 views	spiv15 18 Jan 2013
Protocol for elution of desthiobiotin-labelled proteins from Streptavidin Column Started by lilpalmitate, 11 Jan 2013 Streptavidin, Mass Spectrometry and 2 more...	1 reply 319 views	neuron 17 Jan 2013
BCA Protein Assay and ELISA afterwards Started by mjore, 17 Jan 2013	1 reply 219 views	bob1 17 Jan 2013
blood contamination in tissue Started by Xin, 17 Jan 2013	1 reply 164 views	bob1 17 Jan 2013
minimum recombinant protein amounts for gst pulldown Started by cellgene, 15 Jan 2013 pulldown;, pull down;, gst; and 2 more...	4 replies 216 views	cellgene 15 Jan 2013
Tissue Homogenate Started by Shannah, 14 Jan 2013	1 reply 171 views	bob1 14 Jan 2013
Avoiding PTM Actylation of rProtein Started by Pangea, 03 Jan 2013 PTM	5 replies 253 views	Pangea 07 Jan 2013
PTM in E. coli Started by Pangea, 07 Jan 2013 PTM E.coli	1 reply 170 views	Tabaluga 07 Jan 2013
Low pH Cell Lysis Buffer Started by ahitron, 02 Jan 2013 cell lysis, ph, SDS-PAGE	0 replies 299 views	ahitron 02 Jan 2013
converting protein names protein ids and enterz gene ids Started by mathew, 27 Dec 2012 converting protein name	1 reply 276 views	pcrman 27 Dec 2012
Help! Purifying native protein-2 years and counting... Started by molbio822, 19 Dec 2012 his-tag, flag-tag and 1 more...	4 replies 381 views	mdfenko 27 Dec 2012
SDS solubilisation, Membrane Proteins and Micelle Formation Started by JoeSus, 26 Dec 2012 SDS, MIcelle, membrane, protein and 1 more...	2 replies 353 views	mdfenko 26 Dec 2012
Protein degradation on 2D gels Started by barnacleman, 22 Dec 2012	2 replies 306 views	barnacleman 24 Dec 2012
experience with Invitrogen's EZQ? Started by pathlily, 20 Dec 2012 protein quantitation, ezq and 1 more...	1 reply 250 views	Curtis 23 Dec 2012
dialyse of a protein Started by NDUFB11, 11 Dec 2012 protein purification	4 replies 430 views	NDUFB11 18 Dec 2012