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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Co-Immunoprecipitation - Query Started by mt2, 09 May 2013 Pre-Blocking beads	2 replies 164 views	mt2 10 May 2013
protein extraction and peptide quantification from fat breast tissue Started by Vero36, 04 May 2013 fat microBCA	1 reply 147 views	mdfenko 06 May 2013
Peptide HPLC Sample Prep Started by Kb Homes, 22 Apr 2013 Peptide, purification, HPLC	2 replies 243 views	Vero36 04 May 2013
problem with gel supershift Started by lipeiqiang78, 01 May 2013	0 replies 149 views	lipeiqiang78 01 May 2013
name of a database to search homologous? Started by valeri_a, 30 Apr 2013 sequence, homologies database	1 reply 158 views	mdfenko 30 Apr 2013
N-terminal KDEL-Sequence Started by Max Power, 19 Apr 2013	0 replies 260 views	Max Power 19 Apr 2013
Autoconversion of photoconvertable fluorophore Started by Ahrenhase, 16 Apr 2013	0 replies 258 views	Ahrenhase 16 Apr 2013
Quantitation of protein concentration in rehydration buffer Started by kartiga natarajan, 15 Apr 2013 membrane proteins	3 replies 213 views	mdfenko 16 Apr 2013
SDS-PAGE sample streaking issue Started by littledamn, 04 Apr 2013	8 replies 516 views	littledamn 13 Apr 2013
Membrane proteins on 2D gel electrophoresis..... Started by kartiga natarajan, 05 Apr 2013 Need suggestions for IEF	1 reply 382 views	mdfenko 08 Apr 2013
membrane proteomics Started by biochemscientist, 07 Apr 2013	1 reply 250 views	mdfenko 08 Apr 2013
Rapid western blot buffer ?? Started by ickypimp, 30 Apr 2012	2 replies 577 views	hrx 04 Apr 2013
Antibody detection problem Started by Waqar Ahmad, 03 Apr 2013 Antibody, blotting	1 reply 251 views	mdfenko 03 Apr 2013
Protein quantification using HPLC Started by Ubiquitous, 27 Mar 2013	1 reply 432 views	mdfenko 28 Mar 2013
Isoelectric focusing help Started by SailorNorway, 25 Mar 2013	1 reply 183 views	mdfenko 25 Mar 2013
Ultrasonic bath for cell lysis Started by Vinoth Shanmukam, 17 Jan 2012 Ultrasonic bath, Cell lysis and 1 more...	3 replies 977 views	rhombus 22 Mar 2013
Can you store cell pellets for future nuclear extraction? Started by DrAnt1, 08 Feb 2010	5 replies 1,892 views	memari 20 Mar 2013
Protein-protein crosslinking in yeast Started by ckb, 19 Mar 2013	0 replies 192 views	ckb 19 Mar 2013
fast human protein function catalog? Started by mawson, 16 Mar 2013 proteomics analysis and 1 more...	3 replies 246 views	mawson 18 Mar 2013
In solution digestion Started by navid, 12 Mar 2013	2 replies 197 views	navid 14 Mar 2013
ProteinCenter alternative? Started by proteaMatt, 11 Mar 2013	3 replies 204 views	proteaMatt 12 Mar 2013
low concentration protein purification Started by Loopsq, 08 Mar 2013 rp hplc, purification	1 reply 258 views	mdfenko 11 Mar 2013
endosomal protein western blot Started by kathyoc, 07 Mar 2013 membrane bound	0 replies 175 views	kathyoc 07 Mar 2013
Purification of RNA binding proteins by avidin agarose beads Started by kartiga natarajan, 19 Feb 2013 2D gel electrophoresis	2 replies 257 views	mdfenko 05 Mar 2013
Using centrifugal filter units to concentrate protein Started by science noob, 28 Feb 2013	7 replies 270 views	Missle 04 Mar 2013