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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Insulin level in dot blot Started by wdyeo, 04 Sep 2012	0 replies 322 views	wdyeo 04 Sep 2012
Peptide Conc @215 and 225nm? Started by saad, 04 Sep 2012	1 reply 322 views	proteaMatt 04 Sep 2012
In vitro transcription/translated protein to cells!!! Started by dvpr2012, 23 Sep 2012 In vitro transcription and 1 more...	1 reply 314 views	ascacioc 24 Sep 2012
Problem with subcellular fractionation Started by fruitpunchsamurai, 29 Nov 2012 Cell, fractionation, lamin and 1 more...	1 reply 310 views	$Problem with subcellular fractionation - last post by Curtis$ Curtis 29 Nov 2012
PDCD4 Plasmid, RAW 264.7 not working Help Started by AmitS, 24 Sep 2012	2 replies 309 views	gunes 27 Sep 2012
Protocol for elution of desthiobiotin-labelled proteins from Streptavidin Column Started by lilpalmitate, 11 Jan 2013 Streptavidin, Mass Spectrometry and 2 more...	1 reply 308 views	neuron 17 Jan 2013
Protein degradation on 2D gels Started by barnacleman, 22 Dec 2012	2 replies 301 views	barnacleman 24 Dec 2012
protein-protein interaction Started by zogene, 20 Jan 2013	4 replies 298 views	zogene 30 Jan 2013
Low pH Cell Lysis Buffer Started by ahitron, 02 Jan 2013 cell lysis, ph, SDS-PAGE	0 replies 294 views	ahitron 02 Jan 2013
TFE in inducing alpha-helix formation in proteins Started by vivlee, 30 Oct 2012	3 replies 292 views	HOYAJM 02 Nov 2012
sample preparation from whole cell lysates for Edman degradation Started by suncokret, 18 Sep 2012	2 replies 290 views	suncokret 18 Sep 2012
EMSA: cell extract vs purified protein Started by qpwoei4756, 08 Feb 2013 EMSA, protein	2 replies 282 views	qpwoei4756 19 Feb 2013
Mass-spec compatible silver stain Started by chimera, 04 Dec 2012	2 replies 281 views	casandra 05 Dec 2012
Brief vortexing for reconstitution of recombinant proteins? Started by gunes, 27 Sep 2012 dissolving proteins	1 reply 277 views	mdfenko 27 Sep 2012
Enzyme Kinetics Started by HOYAJM, 08 Nov 2012 kcat km	2 replies 273 views	HOYAJM 12 Nov 2012
converting protein names protein ids and enterz gene ids Started by mathew, 27 Dec 2012 converting protein name	1 reply 271 views	pcrman 27 Dec 2012
CCRK (CDK20) protein expression and western blot Started by kblee, 13 Nov 2012	7 replies 263 views	bob1 19 Nov 2012
Protein stability at pH8.3 Started by Wyanstown, 15 Oct 2012	2 replies 262 views	Wyanstown 16 Oct 2012
immunoprecipitation troubleshooting Started by samuel liao, 28 Oct 2012	1 reply 262 views	gmcstay 29 Oct 2012
protein sample floating out of the tricine gel Started by catalina_roe, 15 Jan 2013 tricine-SDS-PAGE, protein sample	3 replies 261 views	Rosewater 18 Jan 2013
2D Image Primary Cell line Started by virusproteomics, 19 Apr 2013 Primary cell line proteo	9 replies 259 views	mdfenko Yesterday, 11:27 AM
Protein concentration of a labelled membrane protein Started by Vinnie, 27 Jan 2013	2 replies 258 views	Vinnie 28 Jan 2013
rpHPLC chromatograph shift Started by ionchannelbk, 23 Oct 2012 HPLC, protein separation	3 replies 256 views	ionchannelbk 24 Oct 2012
pacyc184 vector Started by proteinz, 18 Sep 2012	0 replies 255 views	proteinz 18 Sep 2012
Histone extraction from brain tissue Started by LacquerHead, 05 Nov 2012 Histone, Brain	1 reply 252 views	HOYAJM 06 Nov 2012