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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 12,008 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 6,487 views	rumi84 15 Apr 2009
His tag protein purification problem Started by sankarmanicka, 12 Jun 2004 1 2	Hot 17 replies 17,133 views	QFBDavid 09 Aug 2010
SDS-PAGE running strangely Started by flashboy, 17 Jun 2004 1 2	Hot 22 replies 15,382 views	@bhijit 20 Aug 2012
FPLC is behaving strangely Started by Luria Bertani, 21 Aug 2009	12 replies 6,177 views	Must_Grow_Bugs 12 Jan 2010
Western blot - Stripping and Reprobing Started by cwong1215, 04 Mar 2005	8 replies 5,778 views	mabusheh 21 Mar 2005
TCA precipitation of proteins Started by dr_fthiebault, 26 Jan 2004	13 replies 5,374 views	Richer 13 Jan 2005
Current Protocol by John Wiley & Son Started by Minnie Mouse, 27 Jan 2009	4 replies 5,185 views	mdfenko 14 Feb 2012
actin MW problem in western blot Started by medstudent1, 18 May 2009	4 replies 4,737 views	medchemgirl 15 Jun 2009
Human collagen type I molecular weight Started by abohollolo, 10 Aug 2009	3 replies 4,669 views	Chakchel 11 Aug 2009
Difference between Nonidet-P40 and Tween20 and TritonX100 Started by MaggieRoara, 19 Mar 2009	7 replies 4,523 views	bob1 03 Sep 2011
CO-IP problem: non specific prot binding to G beads Started by Dimilletronc, 02 Feb 2009	11 replies 4,414 views	Dimilletronc 10 Feb 2009
How to prepare plasma-membrane fraction? Started by victor.m, 29 Jan 2009	6 replies 4,397 views	$How to prepare plasma-membrane fraction? - last post by Curtis$ Curtis 04 Feb 2009
His-tag protein purification - contamination with other... Started by Bednaar, 04 Nov 2004 1 2	Hot 15 replies 4,352 views	Janina 26 Nov 2004
IP problem: antibody binds too few antigen Started by leptopelis, 19 Feb 2009	13 replies 4,198 views	mikew 20 Feb 2009
Weird BSA standard curve for Bradford assay Started by kathyliaw93, 12 Jun 2009	9 replies 4,144 views	azrael201 09 Feb 2011
2d gel analysis software??? Started by cadbury, 28 Jan 2009	6 replies 4,134 views	mdfenko 24 Sep 2009
Protein extraction from mouse organs Started by jiro_killua, 08 Feb 2009	6 replies 3,999 views	madrius1 10 Feb 2009
Antibody storage Started by stardust, 23 Feb 2009	10 replies 3,911 views	Marvilla 13 Oct 2010
What are Na3VO4 and beta-glycerophosphate for in lysis buffer Started by Rose2004, 02 Mar 2005	11 replies 3,896 views	Rose2004 08 Mar 2005
SDF-1(CXCL12) western blot Started by lymphomaW, 02 Jun 2009	13 replies 3,877 views	alex2815 09 Jan 2012
Problem with protein expression using Pichia Pastoris Started by vlitx, 03 May 2010	0 replies 3,848 views	vlitx 03 May 2010
Problem with protein concentration Started by thibault, 08 Jan 2004	11 replies 3,682 views	phdconsult 01 Apr 2004
protein size gradient gel Started by torte, 30 Mar 2009	10 replies 3,674 views	mdfenko 06 Apr 2009
How to check the translation? Started by lna, 28 Jul 2009	8 replies 3,538 views	lna 31 Jul 2009