Protein and Proteomics
Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins
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Pinned problem with amonium sulfate precipitationStarted by amir1979, 17 Apr 2004 |
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Pinned immunoprecipitation protocolStarted by Minnie Mouse, 01 Feb 2009 |
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SDS-PAGE running strangelyStarted by flashboy, 17 Jun 2004 |
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His tag protein purification problemStarted by sankarmanicka, 12 Jun 2004 |
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No protein spots in 2D gel - Silver staining!Started by rachelhauser, 13 Aug 2010 |
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Denaturing Reagent for Western BlotStarted by chicho, 14 May 2010 |
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His-tag protein purification - contamination with other...Started by Bednaar, 04 Nov 2004 |
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TCA precipitation of proteinsStarted by dr_fthiebault, 26 Jan 2004 |
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IP problem: antibody binds too few antigenStarted by leptopelis, 19 Feb 2009 |
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SDF-1(CXCL12) western blotStarted by lymphomaW, 02 Jun 2009 |
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protein not stained by Coomassie BlueStarted by rock&science, 15 Oct 2012 |
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FPLC is behaving strangelyStarted by Luria Bertani, 21 Aug 2009 |
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Problem with protein concentrationStarted by thibault, 08 Jan 2004 |
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What are Na3VO4 and beta-glycerophosphate for in lysis bufferStarted by Rose2004, 02 Mar 2005 |
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CO-IP problem: non specific prot binding to G beadsStarted by Dimilletronc, 02 Feb 2009 |
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FPLC and Protein ChromatographyStarted by Luria Bertani, 17 Aug 2009 |
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step-wise dialysis?Started by bluebird, 15 Oct 2009 |
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Running a western with cell media in gels?Started by cm13, 13 Jan 2011 |
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Antibody storageStarted by stardust, 23 Feb 2009 |
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protein size gradient gelStarted by torte, 30 Mar 2009 |
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Co-immunoprecipitationStarted by Starlight83, 29 Oct 2009 |
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