Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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SDS-PAGE and Western Blotting

799 topics
2,665 replies

Protein Expression and Purification

503 topics
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	Topic	Started By	Stats	Last Post Info
	How subjective image analysis software is? Started by barnacleman, 27 Jun 2009		0 replies 930 views	barnacleman 27 Jun 2009
	Storage of 8M Urea buffer? Started by PhD, 24 Jun 2009		3 replies 2,085 views	T C 27 Jun 2009
	protein degardation during gel filteration Started by vandana, 24 Jun 2009		2 replies 1,602 views	T C 27 Jun 2009
	lysate fractionation techniques Started by jaknight, 24 Jun 2009		1 reply 1,208 views	mdfenko 25 Jun 2009
	Protein solubility improvements? Started by Givi, 23 Jun 2009		5 replies 2,228 views	Givi 25 Jun 2009
	antibody production..does it matter if the protein is denatured or not? Started by PhD, 23 Jun 2009		3 replies 1,577 views	PhD 24 Jun 2009
	western blotting loading control Started by cn981, 21 Jun 2009		5 replies 2,211 views	cn981 23 Jun 2009
	Protein Smear in SDS-PAGE after concentrated? Started by laurence.ylao, 22 Jun 2009		1 reply 1,388 views	mdfenko 22 Jun 2009
	A sad beginning but a good ending? Started by hanming86, 10 Apr 2009		5 replies 2,364 views	kathyliaw93 21 Jun 2009
	How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p Started by victor.m, 19 Jun 2009		1 reply 1,375 views	liweixie 21 Jun 2009
	Immunolotting of actin protein coming at higher MW Started by repeatcell, 18 Jun 2009		1 reply 1,179 views	mdfenko 19 Jun 2009
	Isolation of secreted proteins from culture media Started by kminor, 18 Jun 2009		2 replies 1,641 views	repeatcell 18 Jun 2009
	smear on gel after tca precipitation Started by yja97, 17 Jun 2009		3 replies 1,737 views	mdfenko 18 Jun 2009
	(in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or Started by HLee, 15 Jun 2009		2 replies 1,378 views	HLee 16 Jun 2009
	distinguish protein expression from high homology Started by harmodrie, 16 Jun 2009		1 reply 1,165 views	ixsix 16 Jun 2009
	Pichia pastoris cell lysis Started by AllyLau, 15 Jun 2009		0 replies 1,660 views	AllyLau 15 Jun 2009
	actin MW problem in western blot Started by medstudent1, 18 May 2009		4 replies 4,330 views	medchemgirl 15 Jun 2009
	How to store membrane with primary antibody? Started by Tenuissimus, 13 Jun 2009		5 replies 1,871 views	medchemgirl 15 Jun 2009
	protein RPHPLC nonlinear dose response calibration curve.. Started by alicee!, 14 Jun 2009		0 replies 903 views	alicee! 14 Jun 2009
	Protein Removal from cell lysate Started by drjcroberts, 12 Jun 2009		1 reply 1,309 views	yobou 13 Jun 2009
	Mass Mapping experiment Started by Maggie_M, 13 Jun 2009		0 replies 856 views	Maggie_M 13 Jun 2009
	non-specific band persistent at 29kDa Started by nirupakl, 11 Jun 2009		2 replies 1,309 views	HomeBrew 13 Jun 2009
	Supershift Problems Started by mdnworms, 10 Jun 2009		2 replies 1,167 views	mdnworms 11 Jun 2009
	GSK-3b activity Started by scistudent, 09 Jun 2009		2 replies 1,459 views	scistudent 10 Jun 2009
	Alternative methods to detect protein:protein interaction Started by saad, 06 Jun 2009		3 replies 1,376 views	mdfenko 10 Jun 2009

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