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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Protein quantification using HPLC Started by Ubiquitous, 27 Mar 2013	1 reply 424 views	mdfenko 28 Mar 2013
Isoelectric focusing help Started by SailorNorway, 25 Mar 2013	1 reply 172 views	mdfenko 25 Mar 2013
Ultrasonic bath for cell lysis Started by Vinoth Shanmukam, 17 Jan 2012 Ultrasonic bath, Cell lysis and 1 more...	3 replies 955 views	rhombus 22 Mar 2013
Can you store cell pellets for future nuclear extraction? Started by DrAnt1, 08 Feb 2010	5 replies 1,875 views	memari 20 Mar 2013
Protein-protein crosslinking in yeast Started by ckb, 19 Mar 2013	0 replies 181 views	ckb 19 Mar 2013
fast human protein function catalog? Started by mawson, 16 Mar 2013 proteomics analysis and 1 more...	3 replies 233 views	mawson 18 Mar 2013
In solution digestion Started by navid, 12 Mar 2013	2 replies 177 views	navid 14 Mar 2013
ProteinCenter alternative? Started by proteaMatt, 11 Mar 2013	3 replies 196 views	proteaMatt 12 Mar 2013
low concentration protein purification Started by Loopsq, 08 Mar 2013 rp hplc, purification	1 reply 239 views	mdfenko 11 Mar 2013
endosomal protein western blot Started by kathyoc, 07 Mar 2013 membrane bound	0 replies 164 views	kathyoc 07 Mar 2013
Purification of RNA binding proteins by avidin agarose beads Started by kartiga natarajan, 19 Feb 2013 2D gel electrophoresis	2 replies 242 views	mdfenko 05 Mar 2013
Using centrifugal filter units to concentrate protein Started by science noob, 28 Feb 2013	7 replies 253 views	Missle 04 Mar 2013
Isoelectric focusing Started by Etemadi, 03 Mar 2013 IEF	1 reply 195 views	mdfenko 04 Mar 2013
IP data confusion Started by Biochem_newbie, 01 Mar 2013 IP Western Blot bands	3 replies 209 views	bob1 01 Mar 2013
Protein isolation and lack of sop's Started by timo716, 24 Feb 2013	1 reply 224 views	mdfenko 25 Feb 2013
reason of vertical streaks on gels Started by Veronika, 25 Feb 2013	1 reply 182 views	mdfenko 25 Feb 2013
Other species detected in mass spec results Started by Photoreceptor, 14 Feb 2013	3 replies 221 views	schmim 22 Feb 2013
Determining protein concentration after solubilisation in Triton X-100 Started by Joe101, 21 Feb 2013 bradford, protein, concentration and 2 more...	2 replies 250 views	sam_neuwied 21 Feb 2013
specific phosphorylation Started by simone dorfmueller, 19 Feb 2013 kinase, phosphorylation	1 reply 233 views	bob1 20 Feb 2013
EMSA: cell extract vs purified protein Started by qpwoei4756, 08 Feb 2013 EMSA, protein	2 replies 285 views	qpwoei4756 19 Feb 2013
Conversion of protein from kDa to gram/L Started by harry348, 18 Feb 2013	4 replies 223 views	HOYAJM 18 Feb 2013
Mass Spec Results - mistaken as another isoform? Started by Photoreceptor, 14 Feb 2013	1 reply 180 views	neuron 14 Feb 2013
Protein expression opposite of mRNA expression. Ideas? Started by ProSubzero, 01 Feb 2013 protein, mrna, expression and 1 more...	6 replies 401 views	mhj222 11 Feb 2013
Whole tissue lysis vs. Organelle (chloroplast) lysis Started by nimbus, 07 Feb 2013	0 replies 186 views	nimbus 07 Feb 2013
Test for heparan sulfate functionality? Started by C8H10N4O2, 04 Feb 2013 heparan sulfate, function and 3 more...	1 reply 205 views	jerryshelly1 05 Feb 2013