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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
TFE in inducing alpha-helix formation in proteins Started by vivlee, 30 Oct 2012	3 replies 297 views	HOYAJM 02 Nov 2012
myc-tag antibody makes multiple bands, even in mock & parental cells Started by kblee, 01 Nov 2012	1 reply 228 views	John Forsberg 01 Nov 2012
TAMRA vs. Rhodamine B Started by nbe, 31 Oct 2012 fluorescence, label, tag, TAMRA and 1 more...	0 replies 233 views	nbe 31 Oct 2012
immunoprecipitation troubleshooting Started by samuel liao, 28 Oct 2012	1 reply 262 views	gmcstay 29 Oct 2012
Thrombin - how to use it in the best way? Started by meri, 25 Oct 2012 gst, thrombin and 1 more...	2 replies 243 views	metionina 28 Oct 2012
Acetone Precipitation of proteins Started by Izanloo, 27 Oct 2012	1 reply 222 views	prabhubct 27 Oct 2012
dephosphorylate serines Started by wizzkid, 25 Oct 2012 SAP	1 reply 181 views	John Forsberg 25 Oct 2012
Question with lysis buffer to probe for phospho-cMet receptor Started by crom80, 21 Sep 2012	5 replies 421 views	crom80 25 Oct 2012
rpHPLC chromatograph shift Started by ionchannelbk, 23 Oct 2012 HPLC, protein separation	3 replies 258 views	ionchannelbk 24 Oct 2012
Kinase pulldown Started by ssaputo, 19 Oct 2012 pulldown, kinase, substrate and 1 more...	1 reply 324 views	bob1 19 Oct 2012
Protein stability at pH8.3 Started by Wyanstown, 15 Oct 2012	2 replies 262 views	Wyanstown 16 Oct 2012
Purification of FC tagged proteins Started by Smog187, 27 Sep 2012	3 replies 394 views	Curtis 14 Oct 2012
Software Suggestions - Short Peptides Started by HOYAJM, 01 Oct 2012	3 replies 439 views	HOYAJM 02 Oct 2012
Vesicle immunoprecipitation and Blue Native PAGE Started by flooff, 27 Sep 2012 vesicle, immunoprecipitation and 1 more...	1 reply 385 views	gmcstay 29 Sep 2012
Most annoying Co-IP ever! Halp! Started by Cinisia, 27 Sep 2012 Co-IP	2 replies 449 views	Cinisia 28 Sep 2012
Brief vortexing for reconstitution of recombinant proteins? Started by gunes, 27 Sep 2012 dissolving proteins	1 reply 278 views	mdfenko 27 Sep 2012
PDCD4 Plasmid, RAW 264.7 not working Help Started by AmitS, 24 Sep 2012	2 replies 310 views	gunes 27 Sep 2012
Cleaved GST fusion protein retained on beads! Started by mert1518, 03 Mar 2005	8 replies 2,754 views	cdc2kinase 26 Sep 2012
In vitro transcription/translated protein to cells!!! Started by dvpr2012, 23 Sep 2012 In vitro transcription and 1 more...	1 reply 314 views	ascacioc 24 Sep 2012
Role of Isoleucine in protein structure. Started by le_duy211, 17 Sep 2012	1 reply 332 views	ascacioc 19 Sep 2012
co-IP after ubiquitin cleavage Started by Wickerl3732, 14 Sep 2012 Isopeptidase T, Ubiquitin, co-IP and 1 more...	5 replies 415 views	Wickerl3732 18 Sep 2012
sample preparation from whole cell lysates for Edman degradation Started by suncokret, 18 Sep 2012	2 replies 290 views	suncokret 18 Sep 2012
pacyc184 vector Started by proteinz, 18 Sep 2012	0 replies 255 views	proteinz 18 Sep 2012
Heterologous gene expression in yeast - a bioinformatician needs your help cloni Started by myclight, 12 Sep 2012 yeast, protein expression	1 reply 370 views	myclight 13 Sep 2012
Insoluble material floats over my SDS lysate of human cells after centrifugation Started by scddfmsku, 03 Sep 2012	7 replies 448 views	scddfmsku 11 Sep 2012