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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 6,910 views	rumi84 15 Apr 2009
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 12,653 views	Zela 25 Jan 2012
Surface biotinylation problem(s) Started by German13, 05 Mar 2014 westerns, biotin	1 reply 55 views	GNANA 06 Mar 2014
contamination in mass spectrometric data Started by biochemscientist, 05 Mar 2014	2 replies 59 views	miST32 05 Mar 2014
Precipitation during coupling Started by Dave_Kub_11, 25 Feb 2014	3 replies 107 views	mdfenko 27 Feb 2014
High MW IgG signals in IP/co-IP with protein G Started by miST32, 19 Feb 2014 IgG, Rabbit IgG, Co-IP, IP and 1 more...	1 reply 102 views	miST32 01 Mar 2014
identifying s-glutathionylation NEM Vs DTT Started by rajgene, 19 Feb 2014 NEM DTT, alkylation vs reduction	1 reply 69 views	mdfenko 20 Feb 2014
Can I use methanol fixation to fix cells before proteomic analysis? Started by Benz, 11 Feb 2014 methanol fixation, proteomics	1 reply 123 views	neuron 12 Feb 2014
Please help uneven bands in western blot Started by Soda Yip, 30 Jan 2014 uneven bands, western blot and 1 more...	9 replies 293 views	mdfenko 20 Feb 2014
SNP effect on protein binding affinity Started by Niaz, 16 Jan 2014 Protein interaction, SNP effect	5 replies 152 views	jerryshelly1 21 Jan 2014
Cell Lysate Western Blot Little or No Signal Started by alphawhiskey, 15 Jan 2014	7 replies 249 views	jerryshelly1 17 Jan 2014
blank and substrate control give OD reads Started by intandiana, 15 Jan 2014	0 replies 142 views	intandiana 15 Jan 2014
Help on processing mice ascites (peritoneal fluid) please Started by hsuanorama, 13 Jan 2014 ascites, peritoneal fluid, mice	0 replies 117 views	hsuanorama 13 Jan 2014
Answered Protein concentration and extinction coefficient Started by nitrox, 13 Jan 2014 Protein concentration and 1 more...	2 replies 164 views	nitrox 16 Jan 2014
Western Blot Problems...too many unspecific bands Started by Steffen Mueller, 10 Jan 2014	4 replies 197 views	bob1 13 Jan 2014
Total Protein in COS-7 Cells Started by alphawhiskey, 09 Jan 2014	1 reply 135 views	bob1 09 Jan 2014
I never see the 16kDa band from the seeblue marker? Started by CarrieX, 09 Jan 2014	3 replies 153 views	bob1 12 Jan 2014
Which gauge for proper shear force on certain cell dimension Started by steinpdh, 08 Jan 2014	1 reply 147 views	bob1 08 Jan 2014
Magnetic beads and elution Started by murtaugh, 08 Jan 2014	1 reply 142 views	mdfenko 08 Jan 2014
problem in IP Started by CarrieX, 01 Jan 2014	1 reply 167 views	GNANA 01 Jan 2014
Need to strip the membran before probe the internal control protein Started by CarrieX, 31 Dec 2013	3 replies 205 views	bob1 03 Jan 2014
Brown POD-labelled antibody Started by Shrubal, 22 Dec 2013 antibody, western blot, ELISA and 1 more...	0 replies 236 views	Shrubal 22 Dec 2013
Dot blot anomaly Started by Shrubal, 22 Dec 2013 dot blot, western blot	2 replies 282 views	Shrubal 08 Jan 2014
what kind of acetone should be used for deal with carbon support films Started by Hellen, 18 Dec 2013 acetone, carbon support films	1 reply 257 views	mdfenko 18 Dec 2013
Aggregation Index Experience? Started by Missle, 12 Dec 2013 Protein aggregation	3 replies 184 views	tkf 13 Dec 2013