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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 10,132 views	rumi84 15 Apr 2009
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 17,561 views	Zela 25 Jan 2012
protein sequence mutagenesis Started by Chomolungma, 17 Sep 2016	2 replies 127 views	mdfenko 19 Sep 2016
Mass Spectrometry help needed Started by biochem06, 14 Jun 2016	0 replies 943 views	biochem06 14 Jun 2016
Strange Mass spectrometry results Started by biochem06, 17 May 2016	0 replies 319 views	biochem06 17 May 2016
Protein quantification using HPLC/fluorescence Started by phub11, 10 May 2016 HPLC, fluorescence	2 replies 247 views	phub11 14 May 2016
Answered Effect on protein of low pH? Started by Michael Starr, 20 Apr 2016 buffer, protein, degradation, pH	1 reply 484 views	mdfenko 21 Apr 2016
BCA microplate assay with 595 nm filter? Started by Michal Levy, 06 Apr 2016 BCA Assay protein	1 reply 284 views	Andrea Fortina 15 May 2016
Cannot figure this dot blot thing out Started by Ahrenhase, 05 Apr 2016	2 replies 344 views	mdfenko 07 Apr 2016
Answered Stability of a typical protein at 4oC? Started by Michael Starr, 25 Mar 2016 protein, stability, temperature	1 reply 464 views	mdfenko 28 Mar 2016
Answered Uses of ion exchange chromatography? Started by Michael Starr, 25 Mar 2016 iex	1 reply 355 views	mdfenko 28 Mar 2016
Friendly antibody buffer to prevent precipitation? Started by Michael Starr, 24 Mar 2016 antibodies, buffers and 2 more...	3 replies 403 views	mdfenko 28 Mar 2016
Use of fluorescent stain on IEF gels Started by Missle, 15 Mar 2016 IEF, Fluorescent, fixation	1 reply 361 views	Michael Starr 28 Mar 2016
Answered Use of speedvac to dry in-gel peptide digest before sending for LCMS Started by Meg P. Anula, 14 Mar 2016 speedvac, in-gel digest, lcms and 1 more...	2 replies 488 views	mdfenko 17 Mar 2016
doublets on 2D gel Started by biochem06, 09 Mar 2016	4 replies 385 views	Michael Starr 28 Mar 2016
RNA later and 2D SDS PAGE Started by biochem06, 07 Mar 2016	3 replies 344 views	mdfenko 08 Mar 2016
Peptide quantification Started by johnuknow, 26 Feb 2016	4 replies 396 views	johnuknow 29 Feb 2016
Quantifying bands on PAGE gel with ImageJ Started by Michael Starr, 24 Feb 2016 ImageJ, PAGE, debugging and 1 more...	0 replies 822 views	Michael Starr 24 Feb 2016
Affinity purification glycine elution chemistry Started by Michael Starr, 10 Feb 2016 affinity purification and 1 more...	3 replies 380 views	mdfenko 12 Feb 2016
Zeba buffer exchange low protein yield Started by Michael Starr, 10 Feb 2016 Zeba, protein, yield and 2 more...	6 replies 965 views	Michael Starr 28 Mar 2016
iTRAQ or ELISA? Started by BioPe, 01 Feb 2016 iTRAQ, ELISA, time course and 2 more...	1 reply 456 views	neuron 08 Feb 2016
2D Quant kit Started by biochem06, 25 Jan 2016	2 replies 558 views	Andrea Fortina 16 May 2016
2D PAGE and Western blotting Started by biochem06, 19 Jan 2016 2D western blot	1 reply 496 views	Michael Starr 10 Feb 2016
How to detect specific-protein producing cells? Started by Arash Rostami, 06 Jan 2016	8 replies 616 views	bob1 07 Feb 2016
continual problems with 2d gel electrophoresis Started by biochem06, 05 Jan 2016	3 replies 420 views	mdfenko 06 Jan 2016