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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 12,967 views	rumi84 15 Apr 2009
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 20,931 views	Zela 25 Jan 2012
Storage period of protein Started by Siniya, Yesterday, 05:54 AM	1 reply 36 views	mdfenko Yesterday, 02:57 PM
Reduction of Foam formation Started by Siniya, 17 Apr 2018	1 reply 127 views	mdfenko Yesterday, 03:01 PM
Outer membrane protein extraction Started by Siniya, 17 Apr 2018	1 reply 94 views	mdfenko Yesterday, 02:56 PM
HEPES BUFFER and protein degradation Started by Siniya, 07 Apr 2018	1 reply 195 views	mdfenko 09 Apr 2018
coIP troubleshooting Started by Mad Researcher, 16 Feb 2018 coIP, immunoprecipitation and 3 more...	1 reply 659 views	Trof 17 Feb 2018
Magnetic beads vs Sepharose beads Started by Mad Researcher, 11 Feb 2018 coIP, IP, Sepharose beads and 2 more...	1 reply 327 views	bob1 12 Feb 2018
Membrane and whole cell staining Started by Mad Researcher, 07 Feb 2018 staining, immunohistochemistry and 2 more...	0 replies 680 views	Mad Researcher 07 Feb 2018
Whole brain homogenziation for coIP Started by Mad Researcher, 07 Feb 2018 coIP, whole brain, homogenization and 1 more...	1 reply 411 views	bob1 12 Feb 2018
Immunoprecipitation protocol Started by Mad Researcher, 29 Jan 2018 coIP, immunoprecipitation and 1 more...	2 replies 465 views	Mad Researcher 05 Feb 2018
coIP followed by a Western Blot Started by Mad Researcher, 24 Jan 2018 coIP, Western Blot	4 replies 450 views	Mad Researcher 24 Jan 2018
Answered Reverse coIP Started by Mad Researcher, 27 Dec 2017 co-immunoprecipitation, coIP and 1 more...	5 replies 627 views	bob1 02 Jan 2018
Answered N-terminus tagging and signalP prediction Started by Thomson, 10 Dec 2017 n-terminus, tag, signal peptide and 1 more...	2 replies 444 views	Thomson 11 Dec 2017
Answered how to predict transmembrane protein structure Started by Thomson, 13 Nov 2017 transmembrane protein and 1 more...	7 replies 501 views	El Crazy Xabi 21 Nov 2017
slimy cell paste of recombinant e.coli Started by biotef, 06 Nov 2017	3 replies 429 views	labtastic 09 Nov 2017
n=3 for pSILAC Started by Euterpa12, 14 Sep 2017	1 reply 611 views	bob1 14 Sep 2017
Is there any significant difference between endogenous/ native promoter and cons Started by BIOAWY, 22 Aug 2017	1 reply 732 views	bob1 23 Aug 2017
kDa Started by Doraid, 17 Aug 2017	1 reply 567 views	bob1 17 Aug 2017
What is the definition for Protein Abundance. Started by BIOAWY, 24 Apr 2017	0 replies 1,204 views	BIOAWY 24 Apr 2017
Video contest on stories with protein Started by CreativeScience, 25 Mar 2017 contest, protein, video	0 replies 914 views	CreativeScience 25 Mar 2017
Salting out specific protein Started by Raygoza, 23 Feb 2017 salting-out	1 reply 1,813 views	labtastic 23 Feb 2017
Flow Cytometry vs Western Blotting: can't get the same result. Why? Started by Lfs, 16 Oct 2016	1 reply 1,017 views	bob1 17 Oct 2016
Answered Bradford assay which is the blank? Started by Meg P. Anula, 04 Oct 2016 bradford assay, blank, protein	7 replies 1,048 views	Meg P. Anula 13 Oct 2016
protein sequence mutagenesis Started by Chomolungma, 17 Sep 2016	2 replies 956 views	mdfenko 19 Sep 2016