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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 6,773 views	rumi84 15 Apr 2009
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 12,410 views	Zela 25 Jan 2012
Is NuPAGE Bis-Tris gel for native or denaturing protein? Started by ylu, Today, 08:12 AM	1 reply 31 views	mdfenko Today, 03:08 PM
Troubleshooting with silver staining Started by neuron, 28 Nov 2013	3 replies 92 views	mdfenko Yesterday, 03:30 AM
densitometry Analysis Started by rick112, 27 Nov 2013	4 replies 70 views	rick112 29 Nov 2013
Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent Started by AndrewWP, 27 Nov 2013 immunoprecipitation, western blot	4 replies 88 views	mdfenko 28 Nov 2013
please i need clear steps and guide for bacterial enzyme extraction (sonication) Started by Erinle Kennie, 26 Nov 2013 enzyme extraction, purification and 2 more...	0 replies 87 views	Erinle Kennie 26 Nov 2013
B Per for total bacterial protein extraction Started by JamASharpe, 20 Nov 2013	2 replies 89 views	JamASharpe 22 Nov 2013
IP: specific bands in control lanes Started by noliwatson, 13 Nov 2013 IP, western blot, IgG	0 replies 137 views	noliwatson 13 Nov 2013
Sudden problems with Separating Proteins using SDS Gel Started by Sun86, 13 Nov 2013 Protein, Proteomics and 1 more...	2 replies 151 views	mdfenko 14 Nov 2013
Procedure for De-phosphorylation of Cell Lysate Started by Joe KooL, 11 Nov 2013 dephosphorylation	0 replies 142 views	Joe KooL 11 Nov 2013
Western blotting Started by samita, 09 Nov 2013	4 replies 161 views	mdfenko 12 Nov 2013
Dried blood spots internal standard marker protein Started by biochem06, 30 Oct 2013 dried blood spot, protein and 1 more...	0 replies 166 views	biochem06 30 Oct 2013
protein half-life assesment Started by alchemic, 29 Oct 2013	3 replies 226 views	bob1 30 Oct 2013
MS-compatible methods for blocking agarose beads Started by treesandcars, 10 Oct 2013	1 reply 251 views	neuron 18 Oct 2013
2D gel analysis software Started by biochemscientist, 07 Oct 2013	1 reply 183 views	mdfenko 07 Oct 2013
Protein precipitation method Started by Peakos, 03 Oct 2013	2 replies 199 views	labtastic 11 Nov 2013
Protein extracted from RNAlater tissues is blue... Help! Started by Podge, 03 Oct 2013	2 replies 178 views	Podge 04 Oct 2013
identification of transcription factors binding to DNA Started by sudheer.svss, 30 Sep 2013 DNA, protien	1 reply 188 views	theo22 01 Oct 2013
Differentiating between long and short isoforms of Dopamine D2 Receptor using We Started by Heli, 19 Sep 2013 D2R, Rat Striatum and 1 more...	1 reply 222 views	jerryshelly1 19 Sep 2013
what are albumin associated proteins ?and can you please name them? Started by Amena Baig, 18 Sep 2013	1 reply 174 views	Tabaluga 20 Sep 2013
Which domains are commonly found to mediate protein protein interaction? Started by Cacaucenturion2, 17 Sep 2013 PPI, WD40, domain, coiled coil	1 reply 201 views	Curtis 17 Sep 2013
Disappeared bands after the addition of Sigma P2714 Started by domwiledu, 05 Sep 2013 Usage of Prot. inhibitor and 1 more...	1 reply 173 views	mdfenko 06 Sep 2013
Zero absorbance by Bradford assay for Dialyzed protein Started by Mariam, 02 Sep 2013 bradford assay, Urea, Dialysis and 1 more... 1 2	Hot 18 replies 592 views	Mariam 23 Oct 2013
Bradford assay Started by Mariam, 02 Sep 2013	2 replies 254 views	Mariam 07 Sep 2013