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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 7,133 views	rumi84 15 Apr 2009
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 13,046 views	Zela 25 Jan 2012
problems with 1D SDS PAGE Started by biochem06, 21 Jul 2014	0 replies 105 views	biochem06 21 Jul 2014
Increase in enzyme activity Started by carollee, 16 Jul 2014 enzyme activity	1 reply 133 views	mdfenko 17 Jul 2014
peptide concentration Started by ulujm, 15 Jul 2014 peptide protein	1 reply 80 views	bob1 15 Jul 2014
Protein degradation SDS PAGE Started by biochem06, 08 Jul 2014 protein degradation SDS	5 replies 237 views	biochem06 21 Jul 2014
Protein extraction from RNAlater Started by kmshem, 07 Jul 2014 RNAlater, protein extracion and 1 more...	1 reply 89 views	jerryshelly1 07 Jul 2014
Search for quantitative peptide information in human proteome Started by protonoob, 02 Jul 2014 human, proteomics, proteome, uORF	0 replies 162 views	protonoob 02 Jul 2014
Is it possible to perform an MS analysis on an EMSA? Started by Antonio Sillo Rosato, 30 Jun 2014 MALDI MALDI-TOF EMSA	2 replies 114 views	Antonio Sillo Rosato 01 Jul 2014
Can anyone help solve some troubles with co-immunoprecipitation? Started by lydi, 28 Jun 2014 co-immunoprecipitation and 1 more...	5 replies 151 views	dpo 29 Jun 2014
Alternatives to mouse antibody for mouse tissue Started by blixx, 10 Jun 2014	1 reply 145 views	mdfenko 10 Jun 2014
GST tagged GFP Started by scigal, 03 Jun 2014 degradation, GST, GFP and 2 more...	0 replies 175 views	scigal 03 Jun 2014
Putative ligand binding site formed by dimer. Started by JBB2412, 29 May 2014 protein, docking, dimer	0 replies 261 views	JBB2412 29 May 2014
Help us to improve the protein expression in P. pastoris Started by Haidongtan, 29 May 2014 protein expression yeast	0 replies 164 views	Haidongtan 29 May 2014
Looking for in-house, cheap, reliable, in vitro LPS detection assay Started by IPI, 24 May 2014 LPS detection	0 replies 164 views	IPI 24 May 2014
Subcellular fractionation issues - MS analysis Started by Lauren T, 20 May 2014	1 reply 206 views	$Subcellular fractionation issues - MS analysis - last post by neuron$ neuron 05 Jun 2014
Purification of an antibody: crosslinking of resin-GSH to rGST Started by Moonila, 20 May 2014 GST purification, resin-GSH and 1 more...	0 replies 198 views	Moonila 20 May 2014
protein turn-over Started by kli, 08 May 2014	8 replies 274 views	bob1 21 May 2014
poly ubiquity nation detection Started by yobou, 05 May 2014	2 replies 249 views	yobou 24 May 2014
protein structure visualisation Started by h.botond, 29 Apr 2014 protein, software, visualisation	3 replies 310 views	macrox 07 May 2014
Protein isolation for tissue stored in RNAlater Started by Podge, 22 Apr 2014	1 reply 221 views	Inbox 12 May 2014
detection of metal in protein Started by Hiral, 10 Apr 2014	4 replies 273 views	Hiral 11 Apr 2014
antibody concentration for desalting/conjugating to minileak Started by laur, 05 Apr 2014	0 replies 226 views	laur 05 Apr 2014
Heat the sample during nitrogen evaporation of peptides eluted in ethanol/6 N hy Started by Nicolai, 03 Apr 2014 chromatography and 2 more...	2 replies 270 views	Nicolai 23 Apr 2014
Non-denaturing buffer to extract nuclear protein Started by chimera, 02 Apr 2014	1 reply 177 views	bob1 02 Apr 2014