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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 11,604 views	rumi84 15 Apr 2009
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 19,273 views	Zela 25 Jan 2012
What is the definition for Protein Abundance. Started by BIOAWY, 24 Apr 2017	0 replies 817 views	BIOAWY 24 Apr 2017
Video contest on stories with protein Started by CreativeScience, 25 Mar 2017 contest, protein, video	0 replies 537 views	CreativeScience 25 Mar 2017
Salting out specific protein Started by Raygoza, 23 Feb 2017 salting-out	1 reply 862 views	labtastic 23 Feb 2017
Flow Cytometry vs Western Blotting: can't get the same result. Why? Started by Lfs, 16 Oct 2016	1 reply 694 views	bob1 17 Oct 2016
Answered Bradford assay which is the blank? Started by Meg P. Anula, 04 Oct 2016 bradford assay, blank, protein	7 replies 653 views	Meg P. Anula 13 Oct 2016
protein sequence mutagenesis Started by Chomolungma, 17 Sep 2016	2 replies 629 views	mdfenko 19 Sep 2016
Mass Spectrometry help needed Started by biochem06, 14 Jun 2016	1 reply 1,739 views	rick112 01 Dec 2016
Strange Mass spectrometry results Started by biochem06, 17 May 2016	0 replies 627 views	biochem06 17 May 2016
Protein quantification using HPLC/fluorescence Started by phub11, 10 May 2016 HPLC, fluorescence	2 replies 747 views	phub11 14 May 2016
Answered Effect on protein of low pH? Started by Michael Starr, 20 Apr 2016 buffer, protein, degradation, pH	1 reply 941 views	mdfenko 21 Apr 2016
BCA microplate assay with 595 nm filter? Started by Michal Levy, 06 Apr 2016 BCA Assay protein	1 reply 628 views	Andrea Fortina 15 May 2016
Cannot figure this dot blot thing out Started by Ahrenhase, 05 Apr 2016	2 replies 708 views	mdfenko 07 Apr 2016
Answered Stability of a typical protein at 4oC? Started by Michael Starr, 25 Mar 2016 protein, stability, temperature	1 reply 935 views	mdfenko 28 Mar 2016
Answered Uses of ion exchange chromatography? Started by Michael Starr, 25 Mar 2016 iex	1 reply 738 views	mdfenko 28 Mar 2016
Friendly antibody buffer to prevent precipitation? Started by Michael Starr, 24 Mar 2016 antibodies, buffers and 2 more...	3 replies 798 views	mdfenko 28 Mar 2016
Use of fluorescent stain on IEF gels Started by Missle, 15 Mar 2016 IEF, Fluorescent, fixation	1 reply 746 views	Michael Starr 28 Mar 2016
Answered Use of speedvac to dry in-gel peptide digest before sending for LCMS Started by Meg P. Anula, 14 Mar 2016 speedvac, in-gel digest, lcms and 1 more...	2 replies 932 views	mdfenko 17 Mar 2016
doublets on 2D gel Started by biochem06, 09 Mar 2016	4 replies 777 views	Michael Starr 28 Mar 2016
RNA later and 2D SDS PAGE Started by biochem06, 07 Mar 2016	3 replies 659 views	mdfenko 08 Mar 2016
Peptide quantification Started by johnuknow, 26 Feb 2016	4 replies 675 views	johnuknow 29 Feb 2016
Quantifying bands on PAGE gel with ImageJ Started by Michael Starr, 24 Feb 2016 ImageJ, PAGE, debugging and 1 more...	0 replies 1,050 views	Michael Starr 24 Feb 2016
Affinity purification glycine elution chemistry Started by Michael Starr, 10 Feb 2016 affinity purification and 1 more...	3 replies 574 views	mdfenko 12 Feb 2016
Zeba buffer exchange low protein yield Started by Michael Starr, 10 Feb 2016 Zeba, protein, yield and 2 more...	6 replies 1,949 views	Michael Starr 28 Mar 2016