Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

J Biol Methods calls for papers

Journal of Biological Methods (JBM) is now indexed by PubMed Central (PMC) and PubMed, and is now calling for papers.

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 26,939 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 17,785 views	rumi84 15 Apr 2009
Percentage of Outer Membrane Protein Started by Siniya, 30 May 2018	2 replies 1,704 views	yuvsaha123 19 Sep 2021
GTPase assay Started by Shmuel Silnitsky, 08 Aug 2021	2 replies 396 views	Shmuel Silnitsky 09 Aug 2021
Liquid nitrogen protein extraction ?. Started by jackyjoy123 , 01 Jan 2021	2 replies 1,055 views	christinaroberts 04 Mar 2021
eliminating albumin from samples for western blotting ?. Started by jackyjoy123 , 20 Feb 2021	1 reply 500 views	mdfenko 21 Feb 2021
Standard HCL protein hydrolysis technique without vacuum Started by mariiamaak, 31 Mar 2020 hydrolysis vacuum	1 reply 2,407 views	mdfenko 01 Apr 2020
how to enhance the detection limit of home-made Bradford reagent? Started by yobou, 16 Jan 2020 Bradford reagent	2 replies 1,021 views	mdfenko 19 Jan 2020
What are the advantages of iTRAQ/TMT technology over electrophoresis? Started by larry, 06 Jun 2019	0 replies 403 views	larry 06 Jun 2019
blood contamination Started by ldunning, 05 Mar 2009	3 replies 4,748 views	Zagami Francesco 15 Sep 2018
Problem with protein induction Started by sasa_biotech, 19 Jul 2018 Protein induction	2 replies 1,418 views	sasa_biotech 25 Jul 2018
Immunoprecipitation pH switch Started by Trof, 05 Jul 2018	0 replies 1,355 views	Trof 05 Jul 2018
Biotin buffer interference after Strep-TagXT purification Started by Lightbulb_babies, 23 Jun 2018 Strep Tag XT, BXT	0 replies 2,171 views	Lightbulb_babies 23 Jun 2018
When there is no spectral library, How to build the SRM method with theoretical Started by BIOAWY, 13 May 2018 proteomics, skyline	0 replies 3,273 views	BIOAWY 13 May 2018
Quantification of serum GLUT1 and GLUT4 in PCOS patients Started by harry348, 04 May 2018	0 replies 1,229 views	harry348 04 May 2018
Reduction of Foam formation Started by Siniya, 17 Apr 2018	1 reply 1,435 views	mdfenko 23 Apr 2018
Storage period of protein Started by Siniya, 23 Apr 2018	1 reply 1,209 views	mdfenko 23 Apr 2018
Outer membrane protein extraction Started by Siniya, 17 Apr 2018	1 reply 1,205 views	mdfenko 23 Apr 2018
HEPES BUFFER and protein degradation Started by Siniya, 07 Apr 2018	1 reply 1,451 views	mdfenko 09 Apr 2018
coIP troubleshooting Started by Mad Researcher, 16 Feb 2018 coIP, immunoprecipitation and 3 more...	1 reply 2,072 views	Trof 17 Feb 2018
Whole brain homogenziation for coIP Started by Mad Researcher, 07 Feb 2018 coIP, whole brain, homogenization and 1 more...	1 reply 1,944 views	bob1 12 Feb 2018
Magnetic beads vs Sepharose beads Started by Mad Researcher, 11 Feb 2018 coIP, IP, Sepharose beads and 2 more...	1 reply 2,114 views	bob1 12 Feb 2018
Membrane and whole cell staining Started by Mad Researcher, 07 Feb 2018 staining, immunohistochemistry and 2 more...	0 replies 1,967 views	Mad Researcher 07 Feb 2018
Immunoprecipitation protocol Started by Mad Researcher, 29 Jan 2018 coIP, immunoprecipitation and 1 more...	2 replies 2,132 views	Mad Researcher 05 Feb 2018
coIP followed by a Western Blot Started by Mad Researcher, 24 Jan 2018 coIP, Western Blot	4 replies 1,944 views	Mad Researcher 24 Jan 2018