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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 12,656 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 6,912 views	rumi84 15 Apr 2009
Surface biotinylation problem(s) Started by German13, 05 Mar 2014 westerns, biotin	1 reply 57 views	GNANA 06 Mar 2014
contamination in mass spectrometric data Started by biochemscientist, 05 Mar 2014	2 replies 61 views	miST32 05 Mar 2014
High MW IgG signals in IP/co-IP with protein G Started by miST32, 19 Feb 2014 IgG, Rabbit IgG, Co-IP, IP and 1 more...	1 reply 105 views	miST32 01 Mar 2014
Precipitation during coupling Started by Dave_Kub_11, 25 Feb 2014	3 replies 108 views	mdfenko 27 Feb 2014
IP - crosslinking antibody to dynabeads vs. NHS-acitvated beads? Started by Euterpa12, 29 Aug 2013 IP, MS	2 replies 298 views	miST32 23 Feb 2014
Please help uneven bands in western blot Started by Soda Yip, 30 Jan 2014 uneven bands, western blot and 1 more...	9 replies 295 views	mdfenko 20 Feb 2014
identifying s-glutathionylation NEM Vs DTT Started by rajgene, 19 Feb 2014 NEM DTT, alkylation vs reduction	1 reply 70 views	mdfenko 20 Feb 2014
Can I use methanol fixation to fix cells before proteomic analysis? Started by Benz, 11 Feb 2014 methanol fixation, proteomics	1 reply 125 views	neuron 12 Feb 2014
SNP effect on protein binding affinity Started by Niaz, 16 Jan 2014 Protein interaction, SNP effect	5 replies 154 views	jerryshelly1 21 Jan 2014
Cell Lysate Western Blot Little or No Signal Started by alphawhiskey, 15 Jan 2014	7 replies 250 views	jerryshelly1 17 Jan 2014
Answered Protein concentration and extinction coefficient Started by nitrox, 13 Jan 2014 Protein concentration and 1 more...	2 replies 167 views	nitrox 16 Jan 2014
blank and substrate control give OD reads Started by intandiana, 15 Jan 2014	0 replies 143 views	intandiana 15 Jan 2014
Help on processing mice ascites (peritoneal fluid) please Started by hsuanorama, 13 Jan 2014 ascites, peritoneal fluid, mice	0 replies 118 views	hsuanorama 13 Jan 2014
Western Blot Problems...too many unspecific bands Started by Steffen Mueller, 10 Jan 2014	4 replies 199 views	bob1 13 Jan 2014
I never see the 16kDa band from the seeblue marker? Started by CarrieX, 09 Jan 2014	3 replies 155 views	bob1 12 Jan 2014
Low molecular weight proteins below 30kDa Started by Kambiz, 11 Dec 2013 LMW proteins	3 replies 249 views	labtastic 10 Jan 2014
Total Protein in COS-7 Cells Started by alphawhiskey, 09 Jan 2014	1 reply 136 views	bob1 09 Jan 2014
Which gauge for proper shear force on certain cell dimension Started by steinpdh, 08 Jan 2014	1 reply 149 views	bob1 08 Jan 2014
Magnetic beads and elution Started by murtaugh, 08 Jan 2014	1 reply 144 views	mdfenko 08 Jan 2014
Dot blot anomaly Started by Shrubal, 22 Dec 2013 dot blot, western blot	2 replies 284 views	Shrubal 08 Jan 2014
Need to strip the membran before probe the internal control protein Started by CarrieX, 31 Dec 2013	3 replies 207 views	bob1 03 Jan 2014
IP: specific bands in control lanes Started by noliwatson, 13 Nov 2013 IP, western blot, IgG	1 reply 311 views	CarrieX 01 Jan 2014
problem in IP Started by CarrieX, 01 Jan 2014	1 reply 168 views	GNANA 01 Jan 2014