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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 17,597 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 10,157 views	rumi84 15 Apr 2009
protein sequence mutagenesis Started by Chomolungma, 17 Sep 2016	2 replies 158 views	mdfenko 19 Sep 2016
Protein denaturation Started by itmaybe, 30 Sep 2015 denaturation, protein	5 replies 872 views	Nancy.Lee 01 Sep 2016
Mass Spectrometry help needed Started by biochem06, 14 Jun 2016	0 replies 958 views	biochem06 14 Jun 2016
Strange Mass spectrometry results Started by biochem06, 17 May 2016	0 replies 328 views	biochem06 17 May 2016
2D Quant kit Started by biochem06, 25 Jan 2016	2 replies 565 views	Andrea Fortina 16 May 2016
BCA microplate assay with 595 nm filter? Started by Michal Levy, 06 Apr 2016 BCA Assay protein	1 reply 290 views	Andrea Fortina 15 May 2016
Protein quantification using HPLC/fluorescence Started by phub11, 10 May 2016 HPLC, fluorescence	2 replies 254 views	phub11 14 May 2016
Answered Effect on protein of low pH? Started by Michael Starr, 20 Apr 2016 buffer, protein, degradation, pH	1 reply 507 views	mdfenko 21 Apr 2016
Cannot figure this dot blot thing out Started by Ahrenhase, 05 Apr 2016	2 replies 355 views	mdfenko 07 Apr 2016
Zeba buffer exchange low protein yield Started by Michael Starr, 10 Feb 2016 Zeba, protein, yield and 2 more...	6 replies 982 views	Michael Starr 28 Mar 2016
Use of fluorescent stain on IEF gels Started by Missle, 15 Mar 2016 IEF, Fluorescent, fixation	1 reply 370 views	Michael Starr 28 Mar 2016
protein extraction from lettuce Started by m_biotech, 06 Nov 2015 lettuce, protein extraction and 1 more...	1 reply 1,259 views	Michael Starr 28 Mar 2016
doublets on 2D gel Started by biochem06, 09 Mar 2016	4 replies 393 views	Michael Starr 28 Mar 2016
Answered Uses of ion exchange chromatography? Started by Michael Starr, 25 Mar 2016 iex	1 reply 361 views	mdfenko 28 Mar 2016
Answered Stability of a typical protein at 4oC? Started by Michael Starr, 25 Mar 2016 protein, stability, temperature	1 reply 490 views	mdfenko 28 Mar 2016
Friendly antibody buffer to prevent precipitation? Started by Michael Starr, 24 Mar 2016 antibodies, buffers and 2 more...	3 replies 412 views	mdfenko 28 Mar 2016
Answered Use of speedvac to dry in-gel peptide digest before sending for LCMS Started by Meg P. Anula, 14 Mar 2016 speedvac, in-gel digest, lcms and 1 more...	2 replies 498 views	mdfenko 17 Mar 2016
RNA later and 2D SDS PAGE Started by biochem06, 07 Mar 2016	3 replies 353 views	mdfenko 08 Mar 2016
Peptide quantification Started by johnuknow, 26 Feb 2016	4 replies 404 views	johnuknow 29 Feb 2016
Protein expression Started by JohnE, 15 Oct 2015	1 reply 618 views	Michael Starr 25 Feb 2016
yeast two hybrid 3-AT Started by lyok, 31 Oct 2015	1 reply 464 views	Michael Starr 25 Feb 2016
SDS-PAGE sample streaking issue Started by littledamn, 04 Apr 2013	10 replies 1,458 views	Michael Starr 25 Feb 2016
Quantifying bands on PAGE gel with ImageJ Started by Michael Starr, 24 Feb 2016 ImageJ, PAGE, debugging and 1 more...	0 replies 838 views	Michael Starr 24 Feb 2016