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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 14,245 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 7,468 views	rumi84 15 Apr 2009
Trouble eluting all protein from avidin beads Started by Artemis2007, 24 Feb 2015 immunoprecipitation, avidin beads	1 reply 83 views	neuron 26 Feb 2015
RNA/protein pull down Started by laura77, 19 Feb 2015 RNA oligo, Biotin and 2 more...	0 replies 114 views	laura77 19 Feb 2015
2D gel analysis software Started by biochemscientist, 13 Feb 2015	1 reply 234 views	mdfenko 13 Feb 2015
trypsin digestion of 2D-PAGE protein spot Started by yamchi, 18 Oct 2014	2 replies 403 views	neuron 30 Jan 2015
Protein ID in ESI QTOF - Protocol Started by Glyoxime, 23 Dec 2014 ESIQTOF, DIA and 1 more...	1 reply 875 views	neuron 30 Jan 2015
Sirt1 molecular weight Started by Bi0l0gist, 23 Jan 2015	2 replies 132 views	Bi0l0gist 26 Jan 2015
antibody/DNAconjugation and purification Started by Deepblue, 17 Jan 2015 antibody, oligo, conjugation and 1 more...	1 reply 402 views	CPRES 18 Jan 2015
Help with primary antibody Started by pharmsi, 14 Oct 2014	2 replies 414 views	Deepblue 17 Jan 2015
Answered acidic native PAGE Started by EvilTwin, 16 Jan 2015	1 reply 305 views	mdfenko 16 Jan 2015
How to find the interaction between two proteins Started by jaya2020, 12 Jan 2015	2 replies 292 views	jaya2020 13 Jan 2015
Large protein removal (fractionation) Started by TurtleBob, 21 Dec 2014	6 replies 343 views	$Large protein removal (fractionation) - last post by labtastic$ labtastic 29 Dec 2014
How to optimise my western semi-dry transfer? Started by Podge, 04 Dec 2014	3 replies 357 views	Podge 17 Dec 2014
Help on native PAGE gel and its western blot result Started by mysunking, 03 Dec 2014 western blot, native PAGE and 2 more...	5 replies 513 views	mysunking 08 Dec 2014
Western Blot: protein band did not appeared at the correct location Started by shiwei, 11 Sep 2014 mouse iNOS protein	3 replies 496 views	Podge 04 Dec 2014
Urea effect on mice spleen cells Started by Mariam, 23 Nov 2014 urea, proliferation assay, mice and 1 more...	3 replies 341 views	mdfenko 25 Nov 2014
Band did not migrate on gel electrophoresis (SDS) Started by Timilehin Adunni, 17 Nov 2014 gel electrophoresis, Proteomics; and 1 more...	3 replies 371 views	mdfenko 21 Nov 2014
What is the best antibody for the housekeeping protein (control) in skeletal mus Started by Ubala, 18 Nov 2014	3 replies 256 views	bob1 18 Nov 2014
oligomerisation without dimer Started by rockstar, 11 Nov 2014	1 reply 381 views	mdfenko 13 Nov 2014
Can anyone recommend some immunoprecipitation kits? Started by ionchannelbk, 16 Oct 2014 Protein, antibody and 1 more...	1 reply 968 views	miST32 12 Nov 2014
Spectral count from mass spect data Started by biochemscientist, 10 Nov 2014	0 replies 287 views	biochemscientist 10 Nov 2014
problem with Co-IP Started by jaya2020, 04 Sep 2014	8 replies 633 views	jaya2020 28 Oct 2014
No bands can be seen in my co-IP Started by blixx, 25 Oct 2014	3 replies 365 views	blixx 27 Oct 2014
MAPK/MEK Inhibitor stored incorrectly! Is it still ok? Started by matthew.summers, 13 Oct 2014 MAPK/MAK enzyme	2 replies 415 views	matthew.summers 19 Oct 2014