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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 12,460 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 6,793 views	rumi84 15 Apr 2009
what kind of acetone should be used for deal with carbon support films Started by Hellen, Today, 01:04 AM acetone, carbon support films	0 replies 13 views	Hellen Today, 01:04 AM
Low molecular weight proteins below 30kDa Started by Kambiz, 11 Dec 2013 LMW proteins	2 replies 109 views	Kambiz 16 Dec 2013
Aggregation Index Experience? Started by Missle, 12 Dec 2013 Protein aggregation	3 replies 96 views	tkf 13 Dec 2013
Is NuPAGE Bis-Tris gel for native or denaturing protein? Started by ylu, 04 Dec 2013	1 reply 72 views	mdfenko 04 Dec 2013
Troubleshooting with silver staining Started by neuron, 28 Nov 2013	3 replies 114 views	mdfenko 03 Dec 2013
densitometry Analysis Started by rick112, 27 Nov 2013	4 replies 91 views	rick112 29 Nov 2013
Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent Started by AndrewWP, 27 Nov 2013 immunoprecipitation, western blot	4 replies 118 views	mdfenko 28 Nov 2013
please i need clear steps and guide for bacterial enzyme extraction (sonication) Started by Erinle Kennie, 26 Nov 2013 enzyme extraction, purification and 2 more...	0 replies 115 views	Erinle Kennie 26 Nov 2013
B Per for total bacterial protein extraction Started by JamASharpe, 20 Nov 2013	2 replies 109 views	JamASharpe 22 Nov 2013
Sudden problems with Separating Proteins using SDS Gel Started by Sun86, 13 Nov 2013 Protein, Proteomics and 1 more...	2 replies 170 views	mdfenko 14 Nov 2013
IP: specific bands in control lanes Started by noliwatson, 13 Nov 2013 IP, western blot, IgG	0 replies 160 views	noliwatson 13 Nov 2013
Bradford and triton lysis buffer Started by itaimo, 04 Jul 2011	10 replies 1,865 views	JeremyDNA 12 Nov 2013
Western blotting Started by samita, 09 Nov 2013	4 replies 186 views	mdfenko 12 Nov 2013
Procedure for De-phosphorylation of Cell Lysate Started by Joe KooL, 11 Nov 2013 dephosphorylation	0 replies 161 views	Joe KooL 11 Nov 2013
specific phosphorylation Started by simone dorfmueller, 19 Feb 2013 kinase, phosphorylation	2 replies 386 views	Joe KooL 11 Nov 2013
Protein precipitation method Started by Peakos, 03 Oct 2013	2 replies 215 views	labtastic 11 Nov 2013
protein half-life assesment Started by alchemic, 29 Oct 2013	3 replies 248 views	bob1 30 Oct 2013
Dried blood spots internal standard marker protein Started by biochem06, 30 Oct 2013 dried blood spot, protein and 1 more...	0 replies 189 views	biochem06 30 Oct 2013
Zero absorbance by Bradford assay for Dialyzed protein Started by Mariam, 02 Sep 2013 bradford assay, Urea, Dialysis and 1 more... 1 2	Hot 18 replies 632 views	Mariam 23 Oct 2013
MS-compatible methods for blocking agarose beads Started by treesandcars, 10 Oct 2013	1 reply 271 views	neuron 18 Oct 2013
2D gel analysis software Started by biochemscientist, 07 Oct 2013	1 reply 204 views	mdfenko 07 Oct 2013
Probing protein Started by tintuna, 13 Aug 2013	1 reply 277 views	AquaPlasmid 05 Oct 2013
Protein extracted from RNAlater tissues is blue... Help! Started by Podge, 03 Oct 2013	2 replies 194 views	Podge 04 Oct 2013