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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 12,003 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 6,483 views	rumi84 15 Apr 2009
2D Image Primary Cell line Started by virusproteomics, 19 Apr 2013 Primary cell line proteo	12 replies 290 views	virusproteomics Today, 05:17 AM
His Tagged Recombinant Protein not Recognized in Western Blot Started by Missle, Yesterday, 07:34 AM His Tag, Western Blot, Antibody and 1 more...	0 replies 36 views	Missle Yesterday, 07:34 AM
Protein folding in different species Started by 2xzwei, 17 May 2013	1 reply 67 views	DRT 19 May 2013
Immunoprecipitation- Incubation time with beads Started by Niaz, 17 May 2013 IP	1 reply 76 views	bob1 17 May 2013
desalting resin Started by biotef, 17 May 2013	1 reply 38 views	mdfenko 17 May 2013
Protein activation after purification Started by Makosad, 13 May 2013	4 replies 94 views	Makosad 14 May 2013
Membrane vs. Cytosol Fractionation Started by tabassum.sharma, 03 Apr 2013	2 replies 588 views	labtastic 14 May 2013
immunoprecipitation concept Started by soymilk14, 13 May 2013	4 replies 88 views	soymilk14 14 May 2013
2D gel electrophoresis analysis software reg.... Started by kartiga natarajan, 13 May 2013 Hoefer 2D view....	0 replies 76 views	kartiga natarajan 13 May 2013
Co-Immunoprecipitation - Query Started by mt2, 09 May 2013 Pre-Blocking beads	2 replies 107 views	mt2 10 May 2013
protein extraction and peptide quantification from fat breast tissue Started by Vero36, 04 May 2013 fat microBCA	1 reply 97 views	mdfenko 06 May 2013
Peptide HPLC Sample Prep Started by Kb Homes, 22 Apr 2013 Peptide, purification, HPLC	2 replies 188 views	Vero36 04 May 2013
problem with gel supershift Started by lipeiqiang78, 01 May 2013	0 replies 109 views	lipeiqiang78 01 May 2013
name of a database to search homologous? Started by valeri_a, 30 Apr 2013 sequence, homologies database	1 reply 117 views	mdfenko 30 Apr 2013
N-terminal KDEL-Sequence Started by Max Power, 19 Apr 2013	0 replies 233 views	Max Power 19 Apr 2013
Autoconversion of photoconvertable fluorophore Started by Ahrenhase, 16 Apr 2013	0 replies 232 views	Ahrenhase 16 Apr 2013
Quantitation of protein concentration in rehydration buffer Started by kartiga natarajan, 15 Apr 2013 membrane proteins	3 replies 182 views	mdfenko 16 Apr 2013
SDS-PAGE sample streaking issue Started by littledamn, 04 Apr 2013	8 replies 467 views	littledamn 13 Apr 2013
Membrane proteins on 2D gel electrophoresis..... Started by kartiga natarajan, 05 Apr 2013 Need suggestions for IEF	1 reply 357 views	mdfenko 08 Apr 2013
membrane proteomics Started by biochemscientist, 07 Apr 2013	1 reply 222 views	mdfenko 08 Apr 2013
Rapid western blot buffer ?? Started by ickypimp, 30 Apr 2012	2 replies 561 views	hrx 04 Apr 2013
Antibody detection problem Started by Waqar Ahmad, 03 Apr 2013 Antibody, blotting	1 reply 231 views	mdfenko 03 Apr 2013
Protein quantification using HPLC Started by Ubiquitous, 27 Mar 2013	1 reply 423 views	mdfenko 28 Mar 2013