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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 24,939 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 16,316 views	rumi84 15 Apr 2009
Standard HCL protein hydrolysis technique without vacuum Started by mariiamaak, 31 Mar 2020 hydrolysis vacuum	1 reply 1,395 views	mdfenko 01 Apr 2020
how to enhance the detection limit of home-made Bradford reagent? Started by yobou, 16 Jan 2020 Bradford reagent	2 replies 656 views	mdfenko 19 Jan 2020
What are the advantages of iTRAQ/TMT technology over electrophoresis? Started by larry, 06 Jun 2019	0 replies 134 views	larry 06 Jun 2019
blood contamination Started by ldunning, 05 Mar 2009	3 replies 4,423 views	Zagami Francesco 15 Sep 2018
Problem with protein induction Started by sasa_biotech, 19 Jul 2018 Protein induction	2 replies 1,055 views	sasa_biotech 25 Jul 2018
Immunoprecipitation pH switch Started by Trof, 05 Jul 2018	0 replies 1,029 views	Trof 05 Jul 2018
Biotin buffer interference after Strep-TagXT purification Started by Lightbulb_babies, 23 Jun 2018 Strep Tag XT, BXT	0 replies 1,475 views	Lightbulb_babies 23 Jun 2018
Percentage of Outer Membrane Protein Started by Siniya, 30 May 2018	1 reply 986 views	hops proteon 04 Jun 2018
When there is no spectral library, How to build the SRM method with theoretical Started by BIOAWY, 13 May 2018 proteomics, skyline	0 replies 2,549 views	BIOAWY 13 May 2018
Quantification of serum GLUT1 and GLUT4 in PCOS patients Started by harry348, 04 May 2018	0 replies 949 views	harry348 04 May 2018
Reduction of Foam formation Started by Siniya, 17 Apr 2018	1 reply 1,098 views	mdfenko 23 Apr 2018
Storage period of protein Started by Siniya, 23 Apr 2018	1 reply 925 views	mdfenko 23 Apr 2018
Outer membrane protein extraction Started by Siniya, 17 Apr 2018	1 reply 862 views	mdfenko 23 Apr 2018
HEPES BUFFER and protein degradation Started by Siniya, 07 Apr 2018	1 reply 1,094 views	mdfenko 09 Apr 2018
coIP troubleshooting Started by Mad Researcher, 16 Feb 2018 coIP, immunoprecipitation and 3 more...	1 reply 1,711 views	Trof 17 Feb 2018
Whole brain homogenziation for coIP Started by Mad Researcher, 07 Feb 2018 coIP, whole brain, homogenization and 1 more...	1 reply 1,570 views	bob1 12 Feb 2018
Magnetic beads vs Sepharose beads Started by Mad Researcher, 11 Feb 2018 coIP, IP, Sepharose beads and 2 more...	1 reply 1,651 views	bob1 12 Feb 2018
Membrane and whole cell staining Started by Mad Researcher, 07 Feb 2018 staining, immunohistochemistry and 2 more...	0 replies 1,604 views	Mad Researcher 07 Feb 2018
Immunoprecipitation protocol Started by Mad Researcher, 29 Jan 2018 coIP, immunoprecipitation and 1 more...	2 replies 1,789 views	Mad Researcher 05 Feb 2018
coIP followed by a Western Blot Started by Mad Researcher, 24 Jan 2018 coIP, Western Blot	4 replies 1,542 views	Mad Researcher 24 Jan 2018
Answered Reverse coIP Started by Mad Researcher, 27 Dec 2017 co-immunoprecipitation, coIP and 1 more...	5 replies 2,135 views	bob1 02 Jan 2018
Answered N-terminus tagging and signalP prediction Started by Thomson, 10 Dec 2017 n-terminus, tag, signal peptide and 1 more...	2 replies 2,109 views	Thomson 11 Dec 2017
hemoglobin fraction isolation Started by bmoghimi, 16 Aug 2004	1 reply 2,326 views	$hemoglobin fraction isolation - last post by Zagami Francesco$ Zagami Francesco 09 Dec 2017