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Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of expression of proteins

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Topic	Stats	Last Post Info
Pinned problem with amonium sulfate precipitation Started by amir1979, 17 Apr 2004 1 2	Hot 15 replies 25,673 views	Zela 25 Jan 2012
Pinned immunoprecipitation protocol Started by Minnie Mouse, 01 Feb 2009	2 replies 16,974 views	rumi84 15 Apr 2009
eliminating albumin from samples for western blotting ?. Started by jackyjoy123, 20 Feb 2021	1 reply 101 views	mdfenko 21 Feb 2021
Liquid nitrogen protein extraction ?. Started by jackyjoy123, 01 Jan 2021	2 replies 384 views	jackyjoy123 17 Jan 2021
Standard HCL protein hydrolysis technique without vacuum Started by mariiamaak, 31 Mar 2020 hydrolysis vacuum	1 reply 2,140 views	mdfenko 01 Apr 2020
how to enhance the detection limit of home-made Bradford reagent? Started by yobou, 16 Jan 2020 Bradford reagent	2 replies 789 views	mdfenko 19 Jan 2020
What are the advantages of iTRAQ/TMT technology over electrophoresis? Started by larry, 06 Jun 2019	0 replies 227 views	larry 06 Jun 2019
blood contamination Started by ldunning, 05 Mar 2009	3 replies 4,541 views	Zagami Francesco 15 Sep 2018
Problem with protein induction Started by sasa_biotech, 19 Jul 2018 Protein induction	2 replies 1,208 views	sasa_biotech 25 Jul 2018
Immunoprecipitation pH switch Started by Trof, 05 Jul 2018	0 replies 1,182 views	Trof 05 Jul 2018
Biotin buffer interference after Strep-TagXT purification Started by Lightbulb_babies, 23 Jun 2018 Strep Tag XT, BXT	0 replies 1,764 views	Lightbulb_babies 23 Jun 2018
Percentage of Outer Membrane Protein Started by Siniya, 30 May 2018	1 reply 1,146 views	hops proteon 04 Jun 2018
When there is no spectral library, How to build the SRM method with theoretical Started by BIOAWY, 13 May 2018 proteomics, skyline	0 replies 2,842 views	BIOAWY 13 May 2018
Quantification of serum GLUT1 and GLUT4 in PCOS patients Started by harry348, 04 May 2018	0 replies 1,049 views	harry348 04 May 2018
Reduction of Foam formation Started by Siniya, 17 Apr 2018	1 reply 1,236 views	mdfenko 23 Apr 2018
Storage period of protein Started by Siniya, 23 Apr 2018	1 reply 1,028 views	mdfenko 23 Apr 2018
Outer membrane protein extraction Started by Siniya, 17 Apr 2018	1 reply 1,007 views	mdfenko 23 Apr 2018
HEPES BUFFER and protein degradation Started by Siniya, 07 Apr 2018	1 reply 1,241 views	mdfenko 09 Apr 2018
coIP troubleshooting Started by Mad Researcher, 16 Feb 2018 coIP, immunoprecipitation and 3 more...	1 reply 1,843 views	Trof 17 Feb 2018
Whole brain homogenziation for coIP Started by Mad Researcher, 07 Feb 2018 coIP, whole brain, homogenization and 1 more...	1 reply 1,719 views	bob1 12 Feb 2018
Magnetic beads vs Sepharose beads Started by Mad Researcher, 11 Feb 2018 coIP, IP, Sepharose beads and 2 more...	1 reply 1,858 views	bob1 12 Feb 2018
Membrane and whole cell staining Started by Mad Researcher, 07 Feb 2018 staining, immunohistochemistry and 2 more...	0 replies 1,753 views	Mad Researcher 07 Feb 2018
Immunoprecipitation protocol Started by Mad Researcher, 29 Jan 2018 coIP, immunoprecipitation and 1 more...	2 replies 1,918 views	Mad Researcher 05 Feb 2018
coIP followed by a Western Blot Started by Mad Researcher, 24 Jan 2018 coIP, Western Blot	4 replies 1,716 views	Mad Researcher 24 Jan 2018
Answered Reverse coIP Started by Mad Researcher, 27 Dec 2017 co-immunoprecipitation, coIP and 1 more...	5 replies 2,292 views	bob1 02 Jan 2018