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Protein Expression and Purification

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Topic	Stats	Last Post Info
Peptide recuperation after FactorXa cleavage using GST fusion Started by Clauelena13, 18 Sep 2012 E. coli;, peptide; and 2 more...	1 reply 227 views	protolder 20 Sep 2012
Rosetta-gami B Incubation Time Started by SeanWorkman, 09 Jul 2012	1 reply 226 views	pDNA 16 Jul 2012
Buffer exchange of RecA protein Started by Luke William Cuculis, 09 Jul 2012	0 replies 224 views	Luke William Cuculis 09 Jul 2012
Helppurification of expressed protein Started by Always_student, 10 Mar 2013 protein purification and 4 more...	2 replies 222 views	Always_student 15 Mar 2013
Stable protein expression in Vero cells Started by Guada, 06 Sep 2012 Vero cells, stable cell line	2 replies 221 views	Guada 06 Sep 2012
FPLC - why is it so hard to filter my crude extract? Started by Luria Bertani, 28 Jun 2012	1 reply 218 views	mdfenko 28 Jun 2012
Histone extraction from human tissue Started by pakbiochemist, 02 Aug 2012	0 replies 215 views	pakbiochemist 02 Aug 2012
tag for protein purification Started by Angrysh, 17 Apr 2013	7 replies 215 views	labtastic Yesterday, 05:32 AM
Fractionation of Cytoplasmic and Nuclear proteins Started by alyjan, 19 Jun 2012	0 replies 210 views	alyjan 19 Jun 2012
Interesting IMAC Purified Protein Precipitation Problem Started by Sirandar888, 18 Jan 2013 IMAC, Precipitation, covalent	0 replies 210 views	Sirandar888 18 Jan 2013
Advice on protein purification method Started by mcb2012, 15 Jan 2013	0 replies 207 views	mcb2012 15 Jan 2013
Controls for co-immunoprecipitation Started by lschier, 25 Mar 2013	3 replies 207 views	HOYAJM 27 Mar 2013
Peptide concentration methods Started by Clauelena13, 18 Sep 2012 peptideprecipitation; and 2 more...	0 replies 205 views	Clauelena13 18 Sep 2012
pcDNA overexpression Started by Agonist, 27 Feb 2013	4 replies 205 views	Agonist 27 Feb 2013
Surface biotinylation and subsequent chase-to assess the surface protein turnove Started by GNANA, 29 May 2012	0 replies 204 views	GNANA 29 May 2012
Quick Removal of NaOH Started by AddisonJ, 21 Feb 2013	0 replies 204 views	AddisonJ 21 Feb 2013
Protein puriification using IMAC: flowthrough very slow Started by Bioscientist, 15 Mar 2013	2 replies 204 views	mdfenko 18 Mar 2013
Protein is not Binding to DE52 column material Started by Monita, 10 Apr 2013 DE52, Binding of protein	2 replies 200 views	Monita 15 Apr 2013
Western Blot - Protein Marker Present on Film After Developing Started by Epigeneticist, 07 Mar 2013 Western Blot, Protein Expression	1 reply 192 views	GNANA 07 Mar 2013
Help needed in radioactive calculation Started by jaya2020, 10 Jun 2012	0 replies 191 views	jaya2020 10 Jun 2012
How to prevent albumin masking of urokinase from conditioned media Started by Josip Madunic, 03 Apr 2013	3 replies 191 views	mdfenko 04 Apr 2013
How reliable is enterokinase digestion? Started by Suzanna, 25 Jan 2013	0 replies 188 views	Suzanna 25 Jan 2013
Analytical Ultracentrifugation Started by Vikingwizardguy, 01 Jun 2012	0 replies 186 views	Vikingwizardguy 01 Jun 2012
Protein Expression vector with small tag Started by kirstykay, 14 Jan 2013	1 reply 186 views	HOYAJM 14 Jan 2013
UWM is seeking testers for a new protein expression kit Started by ehuwmfdn, 14 Feb 2013 Protein Expression	0 replies 185 views	ehuwmfdn 14 Feb 2013