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Protein Expression and Purification

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Topic	Stats	Last Post Info
How do I get rid of this contaminant? Started by PhD, 17 Aug 2009	13 replies 3,947 views	Prep! 09 Jun 2010
Is it possible? Harsh sonificiation conditions cause degradation of my protein Started by p3t3r1, 15 Aug 2009	3 replies 2,219 views	p3t3r1 16 Aug 2009
Low Thrombin Cleavage Efficiency Problem Started by archit, 15 Aug 2009	1 reply 1,643 views	T C 17 Aug 2009
Throbin cleavage declines with older protein? Started by Dr Rob, 11 Aug 2009	1 reply 1,718 views	klinmed 13 Aug 2009
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 6,826 views	swanny 02 Sep 2009
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 4,654 views	Kristina @Invitrogen Dynal 09 Sep 2009
Newbie question: how to determine protein concentration after IP? Started by dc984, 05 Aug 2009	8 replies 3,908 views	little mouse 24 Aug 2009
protein extraction using trizol Started by rabos, 03 Aug 2009	1 reply 1,267 views	stardust 04 Aug 2009
High pressure on Ni-NTA beads Started by eva_wall, 03 Aug 2009	2 replies 2,302 views	mdfenko 04 Aug 2009
affinity column problems Started by scary, 03 Aug 2009	1 reply 1,520 views	T C 03 Aug 2009
Desalting protein Started by Anna1984, 30 Jul 2009	7 replies 3,913 views	colic101 16 Sep 2009
Trypsin Expression and Purification Started by element771, 29 Jul 2009	3 replies 2,299 views	element771 30 Jul 2009
seeking help in stable cell line generation Started by jueduimeinv, 28 Jul 2009	3 replies 2,025 views	Dr Teeth 29 Jul 2009
BL 21 cells Started by scary, 24 Jul 2009	4 replies 3,459 views	scary 28 Jul 2009
Thiamine HCl 1M solution? Started by PhD, 24 Jul 2009	2 replies 1,793 views	PhD 26 Jul 2009
Low level of expression Started by ram, 20 Jul 2009	6 replies 3,682 views	ram 27 Jul 2009
dnase treatment before his-tag purification Started by timeracer, 20 Jul 2009	3 replies 2,418 views	Luria Bertani 19 Aug 2009
protein aggregation problem Started by anindya, 15 Jul 2009	3 replies 2,392 views	DaveD 11 Feb 2010
protein precipitates out during concentrating it Started by anindya, 15 Jul 2009	2 replies 1,787 views	DaveD 11 Feb 2010
Protein complex immunoprecipitation using Ab/biotin tandem affinity Started by craigGFFKR, 14 Jul 2009	0 replies 1,167 views	craigGFFKR 14 Jul 2009
how to put in evidence protein after IP Started by gigi, 09 Jul 2009	2 replies 1,618 views	cellcounter 10 Jul 2009
GST-fusion protein purification Started by nirajm, 09 Jul 2009	9 replies 4,238 views	OnceHere 16 Sep 2009
In vitro expression extracts Started by jiajia1987, 05 Jul 2009	0 replies 1,013 views	jiajia1987 05 Jul 2009
IPTG Started by rimal, 02 Jul 2009	2 replies 1,691 views	navin 03 Jul 2009
Protein lysis buffer Started by rimal, 27 Jun 2009	4 replies 2,549 views	bguyonnet 06 May 2010