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Protein Expression and Purification

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Topic	Stats	Last Post Info
How to distinguis soluble from insoluble recombinant proteins produced in E. col Started by csalat, 02 Jul 2012	1 reply 305 views	Papaver 02 Jul 2012
how do GST-tags affect gel filtration results? Started by Kmu, 12 Sep 2012	5 replies 305 views	ascacioc 12 Sep 2012
How to improve enzyme activity? Started by aodonnell311, 19 Mar 2013 enzymes, assays, substrates and 2 more...	2 replies 304 views	aodonnell311 30 Mar 2013
Mouse IgG1 purification - Protein G Started by PaF, 15 Aug 2012 monoclonal Ab and 2 more...	1 reply 302 views	mdfenko 16 Aug 2012
Problems with overnights Started by Smog187, 22 Jan 2013 Overnights	2 replies 301 views	Schevchen 20 Feb 2013
secretory expression in s. pombe Started by researchstruck, 16 Mar 2012 s pombe, nmt1 promoter and 2 more...	0 replies 300 views	researchstruck 16 Mar 2012
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	4 replies 300 views	mdfenko 08 Nov 2012
how much time for prepared gel to keep bfore loading? Started by shivangipurohit, 27 Jun 2012	5 replies 299 views	shivangipurohit 28 Jun 2012
Reversed-Phase chromatography for protein purification Started by goeselen, 13 Jun 2012	2 replies 297 views	goeselen 18 Jun 2012
Which kind of ATP do you usually use in you experiment? Started by Rchqing, 04 Sep 2012 ATP	2 replies 297 views	Rchqing 06 Sep 2012
Can you see GFP after lysis mammalian cells? Started by snowchild, 06 Feb 2013 GFP	5 replies 297 views	snowchild 28 Mar 2013
Problem with protein expression in mammalian cells. Started by aneesh vijayan, 11 Apr 2012	0 replies 294 views	aneesh vijayan 11 Apr 2012
alpha synuclein expression and purification Started by salema, 16 Sep 2012 protein purification, alpha syn and 2 more...	0 replies 292 views	salema 16 Sep 2012
How to distinguish proteins in supernatant of coculture? Started by Appletree, 17 Aug 2010	0 replies 290 views	Appletree 17 Aug 2010
Crosslinking at wrong energy level. Started by aazmaish, 28 Feb 2012 Crosslinking	0 replies 289 views	aazmaish 28 Feb 2012
protein is in pellet and supernatant during inclusion body prep Started by orangee, 13 Jan 2011	0 replies 287 views	orangee 13 Jan 2011
Could anyone help me out with the removal GST tag from my recombinant protein? Started by ana4, 25 Jul 2012	1 reply 285 views	bob1 25 Jul 2012
tag for protein purification Started by Angrysh, 17 Apr 2013	7 replies 284 views	labtastic 22 May 2013
Is anti-His antibody detergent sensitive? Started by Curtis, 30 Jan 2013 His-tag, FLAG	6 replies 282 views	Curtis 06 Feb 2013
Protein Purification from Polyacrylamide Gels Started by PancakeSorting, 14 Jan 2013	3 replies 281 views	Adrian K 17 Jan 2013
Can codon optimization make a big difference in protein expression? Started by chan8, 14 May 2012 codon, optimization, expression and 1 more...	0 replies 275 views	chan8 14 May 2012
Decreased protein yields after successive passaging? Started by jzinckgra , 05 Jul 2012	1 reply 273 views	Adrian K 07 Jul 2012
co-eluted protein with his-tag protein Started by alinud, 23 Jul 2012	1 reply 270 views	Inmost sun 24 Jul 2012
tagged protein size Started by zogene, 30 Jan 2013	10 replies 269 views	Curtis 31 Jan 2013
Sediments/debris/pellet in protein lysate? Started by science noob, 14 Mar 2013	3 replies 269 views	HOYAJM 20 Mar 2013