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Protein Expression and Purification

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Topic	Stats	Last Post Info
Extraction of extended spectrum beta-lactamase proteins Started by roy_tropmed, 11 Apr 2010	0 replies 627 views	roy_tropmed 11 Apr 2010
Protein purification problems - His and Protein G Started by chantalh, 15 Apr 2010	0 replies 620 views	chantalh 15 Apr 2010
KCl vs NaCl in lysis buffer Started by Kimberly Decker, 30 May 2010	0 replies 1,074 views	Kimberly Decker 30 May 2010
Smokey stuff in 293T extract Started by AllenChiu, 30 May 2010	0 replies 609 views	AllenChiu 30 May 2010
Expression in Arctic Express but hell comes after Started by MERCIFULJ, 31 May 2010	0 replies 741 views	MERCIFULJ 31 May 2010
Purification Kits Choices Started by valarie, 20 Jun 2010	0 replies 613 views	valarie 20 Jun 2010
factor Xa cleavage of fusion proteins Started by amc, 22 Jun 2010	0 replies 575 views	amc 22 Jun 2010
Expression and Purification of His-KSI-FLAG-tagged protein Started by Ardelus, 07 Jul 2010	0 replies 424 views	Ardelus 07 Jul 2010
pelB signal siquence not processed after expression in BL21 DE3 Started by krh, 19 Jul 2010	0 replies 535 views	krh 19 Jul 2010
sequence of pEG-KG Started by paramyosin, 23 Jul 2010	0 replies 432 views	paramyosin 23 Jul 2010
DNA after TCA precipitation of culture cells Started by Mr. Naive, 12 Aug 2010	0 replies 389 views	Mr. Naive 12 Aug 2010
How to distinguish proteins in supernatant of coculture? Started by Appletree, 17 Aug 2010	0 replies 290 views	Appletree 17 Aug 2010
LPS purification Started by eeppllaa, 07 Sep 2010	0 replies 351 views	eeppllaa 07 Sep 2010
Mammalian secreted protein disappears after EndoF, TeV treatment Started by ranvi, 08 Sep 2010	0 replies 376 views	ranvi 08 Sep 2010
Best fusion tag for multiple purposes Started by arliss, 24 Sep 2010	0 replies 1,010 views	arliss 24 Sep 2010
Sodium deoxycholate solubility issues Started by Claudia266, 12 Oct 2010	0 replies 486 views	Claudia266 12 Oct 2010
pRARE2 plasmid (from rosetta cell lines) Started by sfl40, 17 Oct 2010	0 replies 510 views	sfl40 17 Oct 2010
Blood cell Started by hellashellas, 18 Oct 2010	0 replies 363 views	hellashellas 18 Oct 2010
GST Extraction: Protein Denatures??? Started by mikelb7, 22 Oct 2010	0 replies 423 views	mikelb7 22 Oct 2010
Co-IPs Woes Started by TerrorTabby, 28 Oct 2010	0 replies 455 views	TerrorTabby 28 Oct 2010
Problem with High Molecular Weight glutenin extraction from wheat for MALDI-TOF Started by matthew.ngkw, 11 Nov 2010	0 replies 337 views	matthew.ngkw 11 Nov 2010
RNAlater and protein activity Started by Aitziber, 25 Nov 2010	0 replies 266 views	Aitziber 25 Nov 2010
Cell Free batch protocol Started by kapp, 07 Dec 2010	0 replies 254 views	kapp 07 Dec 2010
Zebrafish 72 hour post fertilization Started by PCR Novice, 09 Dec 2010	0 replies 262 views	PCR Novice 09 Dec 2010
activity staining of phytase Started by Priya Kuttie, 23 Dec 2010	0 replies 748 views	Priya Kuttie 23 Dec 2010