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Protein Expression and Purification

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Topic	Stats	Last Post Info
Endogenous protein purification Started by Nefthys, 27 May 2009	2 replies 2,249 views	Nefthys 28 May 2009
Trouble with protein expression in E.coli Started by res_org, 16 Jun 2009	2 replies 2,236 views	res_org 18 Jun 2009
Insoluble TEV Protease Started by snig, 07 May 2009	2 replies 2,219 views	dr.Phd 04 Jun 2009
Is it possible? Harsh sonificiation conditions cause degradation of my protein Started by p3t3r1, 15 Aug 2009	3 replies 2,217 views	p3t3r1 16 Aug 2009
primer design for pBAD topo expression kit Started by chrisbelle, 05 Apr 2009	2 replies 2,194 views	chrisbelle 24 Aug 2009
resuspend membrane pellet Started by genz, 19 Mar 2009	2 replies 2,185 views	sudhakar mutyala 24 Apr 2009
collagen precipitation Started by gigi, 01 Mar 2009	2 replies 2,163 views	mdfenko 09 Mar 2009
His-tagged protein runs much faster than expected Started by Araz Zeyniyev, 10 Dec 2009	7 replies 2,127 views	HomeBrew 12 Jan 2010
Troubleshooting GST purification Started by momboys3, 05 Nov 2009	4 replies 2,077 views	ranjeet26 13 Nov 2009
Making Recombinant Proteins Started by Gam8Doc, 13 May 2009	2 replies 2,034 views	Penguin 15 May 2009
no expression of my protein Started by rosepolaris, 06 Feb 2009	1 reply 2,033 views	T C 12 Feb 2009
seeking help in stable cell line generation Started by jueduimeinv, 28 Jul 2009	3 replies 2,015 views	Dr Teeth 29 Jul 2009
Concentrating Protein with centrifuge concentrator but 50% glycerol!!? Started by bc87, 01 Oct 2009	2 replies 2,010 views	Prep! 26 Oct 2009
Kozak consensus sequence for insect expression Started by Christ, 13 Mar 2009	1 reply 1,997 views	klinmed 13 Mar 2009
membrane protein extraction buffer Started by pokemon, 14 Oct 2009	1 reply 1,997 views	Dark Monarch 14 Oct 2009
Microcon troubles Started by dnafactory, 19 Jun 2009	4 replies 1,957 views	mdfenko 22 Jun 2009
Problem with Protein elution using GST sepharose 4B Started by KAUSHIK THAKKAR, 15 Sep 2009	1 reply 1,955 views	paramyosin 07 Oct 2009
Dialysis Started by Juliasarmoire, 19 Apr 2010	6 replies 1,948 views	NHI 13 Jul 2010
Protein extraction from rat aorta Started by kellyly, 14 May 2009	3 replies 1,947 views	JEE 08 Jul 2010
confirming nucleotide state of isolated G protein Started by mmt, 05 Feb 2009	1 reply 1,942 views	rkay447 06 Feb 2009
stabilization of purified protein solutions Started by paramyosin, 09 Mar 2009	2 replies 1,930 views	paramyosin 10 Mar 2009
Commercially available bacterial expression system with chaperone coexpression Started by ssray2000, 03 Mar 2009	1 reply 1,904 views	T C 03 Mar 2009
Backgrounds persistence and higher than target protein in western blot Started by Adrian K, 21 Mar 2011	Hot 14 replies 1,904 views	Adrian K 06 Oct 2011
What is stringent vector? Started by ram, 11 Jun 2009	2 replies 1,900 views	ram 12 Jun 2009
reusing Ni NTA Started by PhD, 24 Aug 2009	2 replies 1,890 views	mdfenko 24 Aug 2009