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Protein Expression and Purification

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Topic	Stats	Last Post Info
Problems with overnights Started by Smog187, 22 Jan 2013 Overnights	2 replies 289 views	Schevchen 20 Feb 2013
problem in extracting the protein Started by An-nur Al-Irsyad, 22 Jan 2013 enzyme	1 reply 244 views	phage434 22 Jan 2013
Interesting IMAC Purified Protein Precipitation Problem Started by Sirandar888, 18 Jan 2013 IMAC, Precipitation, covalent	0 replies 209 views	Sirandar888 18 Jan 2013
Protein purification problems: Presence of contaminated proteins along with the Started by Bioscientist, 16 Jan 2013	5 replies 295 views	Adrian K 20 Jan 2013
Advice on protein purification method Started by mcb2012, 15 Jan 2013	0 replies 207 views	mcb2012 15 Jan 2013
Protein Purification from Polyacrylamide Gels Started by PancakeSorting, 14 Jan 2013	3 replies 270 views	Adrian K 17 Jan 2013
Protein Expression vector with small tag Started by kirstykay, 14 Jan 2013	1 reply 186 views	HOYAJM 14 Jan 2013
Protein precipitated while kept at 4C over night after elution from a Ni-NTA col Started by Blindspot, 14 Jan 2013 NiNTA, precipitation, dialysis and 1 more...	4 replies 388 views	snowchild 28 Mar 2013
Albumin Removal From BSA Started by Hemavet, 10 Jan 2013	1 reply 228 views	Pangea 12 Jan 2013
Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells Started by Pangea, 05 Jan 2013 Large-Scale Purification	7 replies 343 views	Pangea 10 Jan 2013
Insulin production Started by Pangea, 05 Jan 2013 insulin	2 replies 229 views	Pangea 09 Jan 2013
High Yield Protein Expression 10g/L Started by Pangea, 03 Jan 2013 Expression, Protein, Yield	5 replies 342 views	Adrian K 13 Jan 2013
No activity of expressed protein obtained from pET32a cloned gene Started by hRNA, 01 Jan 2013	6 replies 343 views	Adrian K 17 Jan 2013
GST- tagged protein expressed in insoluble Started by biovinz, 31 Dec 2012	5 replies 365 views	biovinz 04 Mar 2013
Scaling up reagents for large scale immunoprecipitation Started by cancerous, 30 Dec 2012 protein A/G, antibody and 2 more...	1 reply 346 views	Curtis 02 Jan 2013
Prepare protein standards for concentration determination Started by KasiaUR, 22 Dec 2012 the BCA method	1 reply 325 views	bob1 23 Dec 2012
Inclusion body formation of the recombinant protein expressed in pET28a in E.col Started by Mehak-khushboo, 04 Dec 2012	3 replies 356 views	Pangea 09 Jan 2013
Tagged recombinant protein has different mobility? Started by An-nur Al-Irsyad, 28 Nov 2012 tagged protein	4 replies 387 views	mdfenko 23 Jan 2013
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	4 replies 283 views	mdfenko 08 Nov 2012
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2	Hot 28 replies 1,030 views	Fluffy 04 Jan 2013
TCA IgG precipitation Started by pablo.barra, 22 Oct 2012 TCA IgG purification	2 replies 240 views	pablo.barra 23 Oct 2012
Does triple labeled protein precipitate more easily? Started by Rchqing, 19 Sep 2012	2 replies 235 views	Rchqing 19 Sep 2012
High gravity crush beads ? Started by @bhijit, 18 Sep 2012 Ni-NTA, Histag	1 reply 242 views	mdfenko 19 Sep 2012
Peptide recuperation after FactorXa cleavage using GST fusion Started by Clauelena13, 18 Sep 2012 E. coli;, peptide; and 2 more...	1 reply 224 views	protolder 20 Sep 2012
Peptide concentration methods Started by Clauelena13, 18 Sep 2012 peptideprecipitation; and 2 more...	0 replies 203 views	Clauelena13 18 Sep 2012