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Protein Expression and Purification

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Topic	Stats	Last Post Info
Problem with Pierce GST spin purification column Started by biolcrazy, 11 Jun 2011	6 replies 1,052 views	biolcrazy 17 Jul 2011
problem in expressing A DNA binding protein Started by gurmeet kaur, 16 Feb 2012	6 replies 600 views	vsoy 12 Jun 2012
Nickel IMAC for His-tag - column goes dark brown! What does this mean? Started by Luria Bertani, 10 Jun 2012	6 replies 640 views	Missle 31 Dec 2012
Multiple protein bands after expression Started by pEXP23, 01 Aug 2012	6 replies 386 views	ascacioc 31 Aug 2012
Enzyme storage in eppendorphs to use in enzyme assay next day Started by San1986, 05 Aug 2012	6 replies 248 views	San1986 09 Aug 2012
No activity of expressed protein obtained from pET32a cloned gene Started by hRNA, 01 Jan 2013	6 replies 343 views	Adrian K 17 Jan 2013
Is anti-His antibody detergent sensitive? Started by Curtis, 30 Jan 2013 His-tag, FLAG	6 replies 270 views	Curtis 06 Feb 2013
C-terminal GST fusion problem in mammalian cell Started by snowchild, 03 Feb 2013 mammalian, protein expression and 1 more...	6 replies 234 views	snowchild 07 Feb 2013
Protein purifcation without chromatography Started by 2xzwei, 23 Apr 2013	6 replies 181 views	mdfenko 23 Apr 2013
pgex Started by saraarasus, 27 Jan 2009	5 replies 3,814 views	swanny 10 Mar 2009
Protein precipitates out so fast Started by shimshady, 09 Apr 2009	5 replies 3,157 views	mdfenko 08 Jun 2009
How to elute this protein?! Started by madrius1, 07 May 2009	5 replies 3,862 views	klinmed 30 Jul 2009
expression vector in E. coli Started by Alie, 05 Jun 2009	5 replies 2,631 views	klinmed 08 Jul 2009
Antibody-beads crosslink Started by dnafactory, 15 Jun 2009	5 replies 3,408 views	dnafactory 24 Aug 2009
Looking for endogenus expression by using custom antibody Started by Gonzo, 07 Dec 2009	5 replies 1,807 views	smu 07 Dec 2009
Expression problem using invitrogen pRSET C vector Started by valarie, 19 Dec 2009	5 replies 3,367 views	Vini 27 Dec 2009
Weird Induction Problem Started by 1324409, 15 Jan 2010	5 replies 1,495 views	1324409 29 Jan 2010
Coupling of protein to NHS activated HP column (GE healthcare)? Started by PhD, 05 Mar 2010	5 replies 1,685 views	mdfenko 09 Mar 2010
Protein precipitates upon dialysis Started by research_freak, 30 Mar 2010	5 replies 1,633 views	ProteinWork 19 Apr 2010
Freezing eluted His tag protein Started by BB25, 03 Jun 2010	5 replies 1,207 views	ram 05 Sep 2010
E.coli expression Started by jal27, 30 Jul 2010	5 replies 886 views	ram 05 Sep 2010
His-tag protein purification Started by deep1985, 16 Jan 2011	5 replies 1,085 views	deep1985 19 Jan 2011
Heterologous protein extraction in yeast for enzymatic activity test Started by simlez, 24 Mar 2011	5 replies 1,308 views	protolder 02 Nov 2011
Problems in recombinant protein expression Started by vels, 20 Apr 2011	5 replies 756 views	Adrian K 04 May 2011
Storage condition of Recombinant protein (native) Started by Adrian K, 21 Apr 2011	5 replies 895 views	Adrian K 22 Apr 2011