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Protein Expression and Purification

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Topic	Stats	Last Post Info
Is anti-His antibody detergent sensitive? Started by Curtis, 30 Jan 2013 His-tag, FLAG	6 replies 266 views	Curtis 06 Feb 2013
killing molecules identification Started by haitham20eg, 02 Feb 2013 killing molecules and 2 more...	0 replies 169 views	haitham20eg 02 Feb 2013
tagged protein size Started by zogene, 30 Jan 2013	10 replies 246 views	Curtis 31 Jan 2013
How reliable is enterokinase digestion? Started by Suzanna, 25 Jan 2013	0 replies 188 views	Suzanna 25 Jan 2013
Protein expression in e.coli Started by neuron, 23 Jan 2013	5 replies 303 views	neuron 24 Jan 2013
Tagged recombinant protein has different mobility? Started by An-nur Al-Irsyad, 28 Nov 2012 tagged protein	4 replies 388 views	mdfenko 23 Jan 2013
problem in extracting the protein Started by An-nur Al-Irsyad, 22 Jan 2013 enzyme	1 reply 244 views	phage434 22 Jan 2013
Protein purification problems: Presence of contaminated proteins along with the Started by Bioscientist, 16 Jan 2013	5 replies 297 views	Adrian K 20 Jan 2013
Interesting IMAC Purified Protein Precipitation Problem Started by Sirandar888, 18 Jan 2013 IMAC, Precipitation, covalent	0 replies 210 views	Sirandar888 18 Jan 2013
Protein Purification from Polyacrylamide Gels Started by PancakeSorting, 14 Jan 2013	3 replies 272 views	Adrian K 17 Jan 2013
No activity of expressed protein obtained from pET32a cloned gene Started by hRNA, 01 Jan 2013	6 replies 343 views	Adrian K 17 Jan 2013
Advice on protein purification method Started by mcb2012, 15 Jan 2013	0 replies 207 views	mcb2012 15 Jan 2013
Protein Expression vector with small tag Started by kirstykay, 14 Jan 2013	1 reply 186 views	HOYAJM 14 Jan 2013
High Yield Protein Expression 10g/L Started by Pangea, 03 Jan 2013 Expression, Protein, Yield	5 replies 342 views	Adrian K 13 Jan 2013
Albumin Removal From BSA Started by Hemavet, 10 Jan 2013	1 reply 229 views	Pangea 12 Jan 2013
Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells Started by Pangea, 05 Jan 2013 Large-Scale Purification	7 replies 344 views	Pangea 10 Jan 2013
Inclusion body formation of the recombinant protein expressed in pET28a in E.col Started by Mehak-khushboo, 04 Dec 2012	3 replies 357 views	Pangea 09 Jan 2013
Insulin production Started by Pangea, 05 Jan 2013 insulin	2 replies 230 views	Pangea 09 Jan 2013
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2	Hot 28 replies 1,035 views	Fluffy 04 Jan 2013
protein with good soulbility but doesnt bind to Ni-NTA coloumn Started by shivu11, 01 Sep 2012 His-tag, protein purification and 1 more...	4 replies 494 views	shivu11 04 Jan 2013
Scaling up reagents for large scale immunoprecipitation Started by cancerous, 30 Dec 2012 protein A/G, antibody and 2 more...	1 reply 347 views	Curtis 02 Jan 2013
Low expression levels of highly soluble protein - why is ON expression not helpi Started by Missle, 15 Jun 2012 Low Expression, E.coli and 1 more...	8 replies 659 views	Missle 31 Dec 2012
Nickel IMAC for His-tag - column goes dark brown! What does this mean? Started by Luria Bertani, 10 Jun 2012	6 replies 637 views	Missle 31 Dec 2012
Prepare protein standards for concentration determination Started by KasiaUR, 22 Dec 2012 the BCA method	1 reply 326 views	bob1 23 Dec 2012
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	4 replies 284 views	mdfenko 08 Nov 2012