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Protein Expression and Purification

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Topic	Stats	Last Post Info
Expression in yeast Started by paramyosin, 06 Oct 2009	0 replies 1,099 views	paramyosin 06 Oct 2009
Truncated or isomer protein Started by anasyida69, 12 Oct 2009	0 replies 1,192 views	anasyida69 12 Oct 2009
Protein Extraction from blood/buffy coat Started by Ben Binks, 15 Oct 2009	0 replies 1,589 views	Ben Binks 15 Oct 2009
Help with T7RNAP purification Started by PaddyS, 16 Oct 2009	0 replies 930 views	PaddyS 16 Oct 2009
how to add NLS to the protein Started by lna, 23 Oct 2009	0 replies 1,082 views	lna 23 Oct 2009
radiolabeling and co-IP with invitrogen dynabeads Started by research_xyz, 24 Oct 2009	0 replies 1,544 views	research_xyz 24 Oct 2009
Bovine presenilin-1-associated protein (PSAP) Started by nirajm, 09 Dec 2009	0 replies 875 views	nirajm 09 Dec 2009
Regeneration of Vivaspin Concentrators Started by Adrian K, 15 Dec 2009	0 replies 1,303 views	Adrian K 15 Dec 2009
Methylation of CMV promoter in HEK cells Started by Benita, 08 Jan 2010	0 replies 864 views	Benita 08 Jan 2010
Transfecting HEK 293as with Pac1 digested Adenoviral vectors from invitrogen... Started by kannan75, 12 Jan 2010	0 replies 742 views	kannan75 12 Jan 2010
membrane protein solubilisation Started by genz, 12 Jan 2010	0 replies 1,170 views	genz 12 Jan 2010
Total protein expression in human dendritic cells Started by jonasns, 15 Jan 2010	0 replies 943 views	jonasns 15 Jan 2010
Protocol for Ni-NTA based protein purification Started by KAUSHIK THAKKAR, 25 Jan 2010	0 replies 1,201 views	KAUSHIK THAKKAR 25 Jan 2010
pH for Ni binding Started by paramyosin, 18 Feb 2010	0 replies 660 views	paramyosin 18 Feb 2010
lost and inactive..protein stuck in pellet:/ Started by Travica, 26 Feb 2010	0 replies 746 views	Travica 26 Feb 2010
pichia expression problems:( Started by gorkem, 26 Feb 2010	0 replies 664 views	gorkem 26 Feb 2010
refolding buffer pH Started by star, 03 Mar 2010	0 replies 814 views	star 03 Mar 2010
eGFP expression level in BL21(DE3) Started by leoyenchenglin, 04 Mar 2010	0 replies 863 views	leoyenchenglin 04 Mar 2010
talon vs NTA Started by genz, 10 Mar 2010	0 replies 640 views	genz 10 Mar 2010
Perotwin precipitation due to zeocin? Started by Benita, 12 Apr 2010	0 replies 653 views	Benita 12 Apr 2010
Extraction of extended spectrum beta-lactamase proteins Started by roy_tropmed, 11 Apr 2010	0 replies 626 views	roy_tropmed 11 Apr 2010
Protein purification problems - His and Protein G Started by chantalh, 15 Apr 2010	0 replies 618 views	chantalh 15 Apr 2010
KCl vs NaCl in lysis buffer Started by Kimberly Decker, 30 May 2010	0 replies 1,068 views	Kimberly Decker 30 May 2010
Smokey stuff in 293T extract Started by AllenChiu, 30 May 2010	0 replies 607 views	AllenChiu 30 May 2010
Expression in Arctic Express but hell comes after Started by MERCIFULJ, 31 May 2010	0 replies 739 views	MERCIFULJ 31 May 2010