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Protein Expression and Purification

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Topic	Stats	Last Post Info
Expression in E.coli Started by ram, 24 Jun 2010 1 2	Hot 16 replies 3,019 views	bhavana 12 Sep 2010
pQE vector protein expression Started by hothot, 02 Apr 2009	3 replies 3,013 views	T C 03 Apr 2009
Role of imidazole in lysis buffer Started by ram, 27 Oct 2009	4 replies 2,961 views	Vini 03 Nov 2009
Ni-NTA beads and dialysis tubing Started by Megan Barker, 05 Oct 2009	4 replies 2,929 views	swanny 22 Nov 2009
remove antibody from eluate in co-immunoprecipitation Started by veronicaalej, 14 Dec 2011 Flag, immunoprecipitate, IgG, MS	2 replies 2,891 views	knuf 22 Dec 2011
Streptactin sepharose purification- binding problem Started by Aj265, 16 Dec 2011 Streptactin	2 replies 2,778 views	Aj265 17 Dec 2011
Plasmid instability in E.coli BL21? Started by chn09, 04 Sep 2009	3 replies 2,767 views	sitting_at _the _AKTA 06 Sep 2009
purification of a basic protein Started by kasturi, 10 Mar 2009	4 replies 2,750 views	kasturi 13 Mar 2009
expression vector in E. coli Started by Alie, 05 Jun 2009	5 replies 2,627 views	klinmed 08 Jul 2009
Inducible expression in human cell line Started by easternblot, 25 Jun 2009	4 replies 2,606 views	IoannaA 24 Feb 2010
Protein lysis buffer Started by rimal, 27 Jun 2009	4 replies 2,541 views	baienoix 06 May 2010
Protein sticks to Ni beads Started by GeneTurk, 09 Apr 2009	2 replies 2,520 views	sudhakar mutyala 24 Apr 2009
Showed a bigger and broad smear of protein!! Started by Natthiya, 03 Mar 2009	4 replies 2,506 views	paramyosin 09 Mar 2009
Nickel IMAC - How to avoid unwanted proteins? Started by thommyd87, 11 May 2009	4 replies 2,446 views	thommyd87 16 May 2009
dnase treatment before his-tag purification Started by timeracer, 20 Jul 2009	3 replies 2,407 views	Luria Bertani 19 Aug 2009
how to isolate insoluble protein in bacterial induction? Started by leomap, 10 Sep 2009	4 replies 2,395 views	Yar 05 Oct 2009
protein aggregation problem Started by anindya, 15 Jul 2009	3 replies 2,389 views	DaveD 11 Feb 2010
Electroelution with no electroeluter Started by PhD, 24 Aug 2009	3 replies 2,314 views	PhD 27 Aug 2009
High pressure on Ni-NTA beads Started by eva_wall, 03 Aug 2009	2 replies 2,297 views	mdfenko 04 Aug 2009
Trypsin Expression and Purification Started by element771, 29 Jul 2009	3 replies 2,296 views	element771 30 Jul 2009
Is My Protein Pure or Not? Started by rpjkmust916, 11 Feb 2011 1 2	Hot 19 replies 2,287 views	rpjkmust916 23 Feb 2011
Protein analog Started by anasyida69, 18 Oct 2009	1 reply 2,268 views	Prep! 26 Oct 2009
problems with detecting protein expression Started by cheehing2, 18 Mar 2009	4 replies 2,262 views	GeneTurk 09 Apr 2009
Sticky Insect cell lysate blocked Ni_NTA column Started by Christ, 12 Mar 2009	2 replies 2,252 views	klinmed 13 Mar 2009
Checking For Protein Expression Started by bkool4ya, 17 Aug 2009	3 replies 2,252 views	Doc Sheldon 04 Sep 2009