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Protein Expression and Purification

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Topic	Stats	Last Post Info
Elimination of the polyethylenimine Started by paramyosin, 24 Mar 2009	8 replies 3,136 views	icemare 16 Oct 2012
Newbie question: how to determine protein concentration after IP? Started by dc984, 05 Aug 2009	8 replies 3,899 views	little mouse 24 Aug 2009
Protein expression Started by bhavana, 03 Sep 2010	8 replies 1,208 views	Prep! 22 Sep 2010
Protein Migration Problem [S.O.S.] Started by Adrian K, 28 Dec 2010	8 replies 1,248 views	mdfenko 13 Jan 2011
how to purify Fc fragment Started by BioMiha, 30 Jun 2011	8 replies 1,236 views	redbio 17 Aug 2011
stop codon readthrough in E.coli Started by Biogareth, 30 Aug 2011 stop codon	8 replies 1,700 views	Biogareth 02 Sep 2011
Immunoprecipitation---Why would a 250kDa band be present for the purification of Started by negrona29, 14 Feb 2012 Immunoprecipitation and 1 more...	8 replies 720 views	negrona29 20 Feb 2012
Low expression levels of highly soluble protein - why is ON expression not helpi Started by Missle, 15 Jun 2012 Low Expression, E.coli and 1 more...	8 replies 655 views	Missle 31 Dec 2012
How to estimate the protein expression level from the SDS_PAGE? Started by crusoe, 01 Apr 2013 expression level, protein	8 replies 299 views	crusoe 06 Apr 2013
Who know anything about dialysis on protein extractions ? Started by Snoopy, 15 Mar 2009	7 replies 3,496 views	sudhakar mutyala 24 Apr 2009
Desalting protein Started by Anna1984, 30 Jul 2009	7 replies 3,897 views	colic101 16 Sep 2009
His-tagged protein runs much faster than expected Started by Araz Zeyniyev, 10 Dec 2009	7 replies 2,127 views	HomeBrew 12 Jan 2010
His tag protein purification Started by kcchalmers, 23 Mar 2011	7 replies 1,009 views	mdfenko 28 Mar 2011
Thrombin cleaved GST-tagged protein remained bound to beads! Started by ntgxw, 25 Apr 2011	7 replies 917 views	mdfenko 29 Apr 2011
Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells Started by Pangea, 05 Jan 2013 Large-Scale Purification	7 replies 338 views	Pangea 10 Jan 2013
Low level of expression Started by ram, 20 Jul 2009	6 replies 3,663 views	ram 27 Jul 2009
membrane protein purification Started by jasmina, 16 Feb 2010	6 replies 1,445 views	jasmina 17 Feb 2010
His Tag Protein Purification Started by wildcatterp, 22 Mar 2010	6 replies 1,401 views	sumogirlie 09 Apr 2010
Expressing 4 proteins in an operon Started by orang, 08 Apr 2010	6 replies 1,343 views	orang 08 Apr 2010
Dialysis Started by Juliasarmoire, 19 Apr 2010	6 replies 1,946 views	NHI 13 Jul 2010
How to normalize my samples before I transfect my cells Started by new2science, 02 May 2010	6 replies 1,485 views	new2science 03 May 2010
Looking for precipitation method to reduce the volume of my protein complex elu Started by AllenChiu, 09 Sep 2010	6 replies 840 views	rkay447 10 Sep 2010
volume to load sample for affinity chromatography Started by rpjkmust916, 10 Feb 2011	6 replies 938 views	rpjkmust916 14 Feb 2011
Low expression of pET21a construct Started by ZF1807, 25 May 2011	6 replies 937 views	qzlabs 12 Jul 2011
Problem with Pierce GST spin purification column Started by biolcrazy, 11 Jun 2011	6 replies 1,046 views	biolcrazy 17 Jul 2011