Protein Expression and Purification

Pinned  a few questions about ion exchange chromatography

• 1
• 2
• 3

Pinned  protein expression in Pichia pastoris

• 1
• 2

pET14b vector with insert transformed in BL21(DE3) RIPL cells

• 1
• 2

Is My Protein Pure or Not?

• 1
• 2

protein expression (temperature issue)

• 1
• 2

Couple questions about GST purification

• 1
• 2

Expression in E.coli

• 1
• 2

protein expression

• 1
• 2

No activity of fungi protein in E.coli

Started by Biogareth, 13 Oct 2011   heteroexpression

• 1
• 2

expression in minimal media

Backgrounds persistence and higher than target protein in western blot

How do I get rid of this contaminant?

Polysorbate 80 (Tween 80) removal

Immunoprecipitation

AnionExchange problem: proteins do not bind

Expressing human protein in E.Coli

HIS-tagged protein purification

Protein doesn't bind any column except Ni

How to purify unstable protein?

tagged protein size

GST-fusion protein purification

Low expression: What else can I do?

ways to stop soluble aggregation

Ammonium sulphate precipitation Problem

protein yield is very low after purification