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Protein Expression and Purification

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Topic	Stats	Last Post Info
Role of imidazole in lysis buffer Started by ram, 27 Oct 2009	4 replies 2,961 views	Vini 03 Nov 2009
BL21A or simliar Started by collitchboy, 29 Oct 2009	2 replies 1,756 views	collitchboy 30 Oct 2009
Protein analog Started by anasyida69, 18 Oct 2009	1 reply 2,268 views	Prep! 26 Oct 2009
Concentrating Protein with centrifuge concentrator but 50% glycerol!!? Started by bc87, 01 Oct 2009	2 replies 2,010 views	Prep! 26 Oct 2009
radiolabeling and co-IP with invitrogen dynabeads Started by research_xyz, 24 Oct 2009	0 replies 1,546 views	research_xyz 24 Oct 2009
how to add NLS to the protein Started by lna, 23 Oct 2009	0 replies 1,083 views	lna 23 Oct 2009
Help with T7RNAP purification Started by PaddyS, 16 Oct 2009	0 replies 931 views	PaddyS 16 Oct 2009
Protein Extraction from blood/buffy coat Started by Ben Binks, 15 Oct 2009	0 replies 1,591 views	Ben Binks 15 Oct 2009
membrane protein extraction buffer Started by pokemon, 14 Oct 2009	1 reply 1,997 views	Dark Monarch 14 Oct 2009
Truncated or isomer protein Started by anasyida69, 12 Oct 2009	0 replies 1,192 views	anasyida69 12 Oct 2009
Problem with Protein elution using GST sepharose 4B Started by KAUSHIK THAKKAR, 15 Sep 2009	1 reply 1,955 views	paramyosin 07 Oct 2009
Expression in yeast Started by paramyosin, 06 Oct 2009	0 replies 1,101 views	paramyosin 06 Oct 2009
how to isolate insoluble protein in bacterial induction? Started by leomap, 10 Sep 2009	4 replies 2,397 views	Yar 05 Oct 2009
293T electroporation Started by laurequillo, 30 Sep 2009	0 replies 1,319 views	laurequillo 30 Sep 2009
pSecTag Expression of a protein with Signal Anchor Started by Benita, 29 Sep 2009	0 replies 1,564 views	Benita 29 Sep 2009
protein is degraded after purification Started by pina, 25 Sep 2009	2 replies 1,388 views	pina 29 Sep 2009
GST-fusion protein purification Started by nirajm, 09 Jul 2009	9 replies 4,235 views	OnceHere 16 Sep 2009
Desalting protein Started by Anna1984, 30 Jul 2009	7 replies 3,904 views	colic101 16 Sep 2009
Problems purifying a protein expressed in Pichia Started by Pichia, 15 Sep 2009	0 replies 1,005 views	Pichia 15 Sep 2009
Low expression: What else can I do? Started by Ivanov_br, 23 Aug 2009	9 replies 3,439 views	Doc Sheldon 14 Sep 2009
eluting antigen Started by dnafactory, 09 Sep 2009	0 replies 1,038 views	dnafactory 09 Sep 2009
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 4,625 views	Kristina @Invitrogen Dynal 09 Sep 2009
Plasmid instability in E.coli BL21? Started by chn09, 04 Sep 2009	3 replies 2,767 views	sitting_at _the _AKTA 06 Sep 2009
Checking For Protein Expression Started by bkool4ya, 17 Aug 2009	3 replies 2,253 views	Doc Sheldon 04 Sep 2009
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 6,792 views	swanny 02 Sep 2009