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Protein Expression and Purification

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Topic	Stats	Last Post Info
EDTA bacterial cell lysate Started by Danny Chow, 03 Jan 2010	2 replies 1,335 views	Danny Chow 12 Jan 2010
membrane protein solubilisation Started by genz, 12 Jan 2010	0 replies 1,171 views	genz 12 Jan 2010
Transfecting HEK 293as with Pac1 digested Adenoviral vectors from invitrogen... Started by kannan75, 12 Jan 2010	0 replies 744 views	kannan75 12 Jan 2010
His-tagged protein runs much faster than expected Started by Araz Zeyniyev, 10 Dec 2009	7 replies 2,129 views	HomeBrew 12 Jan 2010
Methylation of CMV promoter in HEK cells Started by Benita, 08 Jan 2010	0 replies 864 views	Benita 08 Jan 2010
Protein expression using invitrogen pSecTag2 Started by SDP, 16 Dec 2009	2 replies 1,609 views	SDP 07 Jan 2010
Protein Purification from Qiagen RNeasy Kit Started by thebigpiro, 05 Jan 2010	3 replies 1,590 views	jah 05 Jan 2010
from pet-SUMO to pet-28 Started by tatui, 05 Jan 2010	2 replies 1,464 views	tatui 05 Jan 2010
Trouble With HIS affinity column (Ni+) binding Started by ithryn, 31 Dec 2009	1 reply 1,231 views	pDNA 31 Dec 2009
Problems with affinity purification with M2 beads Started by Mylando, 29 Dec 2009	2 replies 1,383 views	Mylando 31 Dec 2009
protein going to inclusion bodies Started by HMG, 08 Dec 2009	1 reply 1,220 views	KAUSHIK THAKKAR 29 Dec 2009
Vector containing N-his tag and TEV Started by qinghong, 16 Dec 2009	1 reply 1,754 views	pDNA 27 Dec 2009
Expression problem using invitrogen pRSET C vector Started by valarie, 19 Dec 2009	5 replies 3,367 views	Vini 27 Dec 2009
Problems with nuclear extraction Started by Kornelis, 15 Dec 2009	1 reply 1,481 views	aimikins 15 Dec 2009
Regeneration of Vivaspin Concentrators Started by Adrian K, 15 Dec 2009	0 replies 1,303 views	Adrian K 15 Dec 2009
Removing phosphates Started by Gonzo, 10 Dec 2009	2 replies 1,578 views	Gonzo 11 Dec 2009
Bovine presenilin-1-associated protein (PSAP) Started by nirajm, 09 Dec 2009	0 replies 875 views	nirajm 09 Dec 2009
Looking for endogenus expression by using custom antibody Started by Gonzo, 07 Dec 2009	5 replies 1,807 views	smu 07 Dec 2009
Adventitious chemical binding Started by Jor5033, 23 Nov 2009	2 replies 1,629 views	StevieRay 24 Nov 2009
Ni-NTA beads and dialysis tubing Started by Megan Barker, 05 Oct 2009	4 replies 2,930 views	swanny 22 Nov 2009
More than one protein induced Started by dora1, 18 Nov 2009	4 replies 1,638 views	Vini 18 Nov 2009
water withdrawal with PEG20.000 Started by LouiseD, 13 Nov 2009	1 reply 1,278 views	mdfenko 13 Nov 2009
Troubleshooting GST purification Started by momboys3, 05 Nov 2009	4 replies 2,080 views	ranjeet26 13 Nov 2009
protein expression Started by chn09, 15 Sep 2009 1 2	Hot 15 replies 5,653 views	ram 05 Nov 2009
Amount of inoculum? Started by ram, 03 Nov 2009	3 replies 1,877 views	ram 05 Nov 2009