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Protein Expression and Purification

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Topic	Stats	Last Post Info
How to prevent albumin masking of urokinase from conditioned media Started by Josip Madunic, 03 Apr 2013	3 replies 191 views	mdfenko 04 Apr 2013
How to improve enzyme activity? Started by aodonnell311, 19 Mar 2013 enzymes, assays, substrates and 2 more...	2 replies 274 views	aodonnell311 30 Mar 2013
Protein precipitated while kept at 4C over night after elution from a Ni-NTA col Started by Blindspot, 14 Jan 2013 NiNTA, precipitation, dialysis and 1 more...	4 replies 389 views	snowchild 28 Mar 2013
Can you see GFP after lysis mammalian cells? Started by snowchild, 06 Feb 2013 GFP	5 replies 283 views	snowchild 28 Mar 2013
Partial cleavage of HA (Hemagglutinin) tag? Started by ACL, 20 Feb 2013	4 replies 234 views	snowchild 28 Mar 2013
Controls for co-immunoprecipitation Started by lschier, 25 Mar 2013	3 replies 207 views	HOYAJM 27 Mar 2013
Sediments/debris/pellet in protein lysate? Started by science noob, 14 Mar 2013	3 replies 251 views	HOYAJM 20 Mar 2013
Protein puriification using IMAC: flowthrough very slow Started by Bioscientist, 15 Mar 2013	2 replies 204 views	mdfenko 18 Mar 2013
Helppurification of expressed protein Started by Always_student, 10 Mar 2013 protein purification and 4 more...	2 replies 222 views	Always_student 15 Mar 2013
Expression and Lysozyme Started by mike79, 13 Jul 2012	3 replies 392 views	Missle 15 Mar 2013
Cleavage of GST-tag with Factor Xa Started by Kmu, 13 Mar 2013	1 reply 148 views	PhDinAcronyms 14 Mar 2013
Trouble in GST- fusion protein purification Started by gdbiomed, 10 Mar 2013	1 reply 170 views	jerryshelly1 13 Mar 2013
protein expression by changing start codon Started by chn09, 09 Mar 2013	1 reply 179 views	bob1 09 Mar 2013
Western Blot - Protein Marker Present on Film After Developing Started by Epigeneticist, 07 Mar 2013 Western Blot, Protein Expression	1 reply 192 views	GNANA 07 Mar 2013
GST- tagged protein expressed in insoluble Started by biovinz, 31 Dec 2012	5 replies 365 views	biovinz 04 Mar 2013
Odd secondary pellet formed after lysis with B-Per or BugBuster Started by Missle, 01 Mar 2013 Bacterial Lysis, Lysis Reagents and 1 more...	2 replies 177 views	Missle 04 Mar 2013
pcDNA overexpression Started by Agonist, 27 Feb 2013	4 replies 205 views	Agonist 27 Feb 2013
membrane proteins ultrafiltration Started by pakbiochemist, 24 Feb 2013	2 replies 181 views	HOYAJM 26 Feb 2013
Quick Removal of NaOH Started by AddisonJ, 21 Feb 2013	0 replies 204 views	AddisonJ 21 Feb 2013
cytoplasmic and nuclear extraction Started by silvepa23, 20 Feb 2013	0 replies 148 views	silvepa23 20 Feb 2013
Problems with overnights Started by Smog187, 22 Jan 2013 Overnights	2 replies 289 views	Schevchen 20 Feb 2013
Very small peptide expression in E. coli Started by Kb Homes, 05 Feb 2013 peptide, expression, his-tag and 1 more...	2 replies 235 views	andy_lord 20 Feb 2013
UWM is seeking testers for a new protein expression kit Started by ehuwmfdn, 14 Feb 2013 Protein Expression	0 replies 185 views	ehuwmfdn 14 Feb 2013
Interferon Alpha Started by Pangea, 08 Feb 2013 interferon	1 reply 230 views	doxorubicin 08 Feb 2013
C-terminal GST fusion problem in mammalian cell Started by snowchild, 03 Feb 2013 mammalian, protein expression and 1 more...	6 replies 230 views	snowchild 07 Feb 2013