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Protein Expression and Purification

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Topic	Stats	Last Post Info
Could anyone help me out with the removal GST tag from my recombinant protein? Started by ana4, 25 Jul 2012	1 reply 274 views	bob1 25 Jul 2012
co-eluted protein with his-tag protein Started by alinud, 23 Jul 2012	1 reply 264 views	Inmost sun 24 Jul 2012
Help with His-Tag protein purification Started by Meyas, 20 Jul 2012 HisTag, Protein purificatio and 3 more...	2 replies 373 views	mzieli 23 Jul 2012
Discrepancy between tissue and cell culture Started by MolecularBiology, 20 Jul 2012 molecular biology, phosphatase	1 reply 248 views	bob1 20 Jul 2012
Rosetta-gami B Incubation Time Started by SeanWorkman, 09 Jul 2012	1 reply 226 views	pDNA 16 Jul 2012
Buffer exchange of RecA protein Started by Luke William Cuculis, 09 Jul 2012	0 replies 221 views	Luke William Cuculis 09 Jul 2012
Decreased protein yields after successive passaging? Started by jzinckgra , 05 Jul 2012	1 reply 268 views	Adrian K 07 Jul 2012
How to distinguis soluble from insoluble recombinant proteins produced in E. col Started by csalat, 02 Jul 2012	1 reply 295 views	Papaver 02 Jul 2012
plant extraction procedure for protein Started by shivangipurohit, 27 Jun 2012	3 replies 232 views	proteaMatt 29 Jun 2012
how much time for prepared gel to keep bfore loading? Started by shivangipurohit, 27 Jun 2012	5 replies 294 views	shivangipurohit 28 Jun 2012
Lysis Buffer turns yellow after phos inhib added. Started by Kate Lee, 27 Jun 2012 lysis, buffer, yellow, problem and 1 more...	1 reply 371 views	mdfenko 28 Jun 2012
FPLC - why is it so hard to filter my crude extract? Started by Luria Bertani, 28 Jun 2012	1 reply 218 views	mdfenko 28 Jun 2012
can Protamine sulfate inhibit or degrage a protein? Started by mbsea, 27 Jun 2012	1 reply 253 views	mdfenko 28 Jun 2012
brain tissue homogenization with sample buffer?? Started by hanbecky, 19 Jun 2012	2 replies 256 views	hanbecky 19 Jun 2012
Fractionation of Cytoplasmic and Nuclear proteins Started by alyjan, 19 Jun 2012	0 replies 210 views	alyjan 19 Jun 2012
Cell lysis buffer for His-pull down Started by Mark_lab, 19 Jun 2012 Pull-down, Histidine and 2 more...	0 replies 294 views	Mark_lab 19 Jun 2012
Reversed-Phase chromatography for protein purification Started by goeselen, 13 Jun 2012	2 replies 294 views	goeselen 18 Jun 2012
rare codon usage in e coli expression very urgent!!!!!!& Started by mbsea, 08 Jun 2012	4 replies 348 views	mbsea 13 Jun 2012
problem in expressing A DNA binding protein Started by gurmeet kaur, 16 Feb 2012	6 replies 600 views	vsoy 12 Jun 2012
protein extraction from human tissue Started by shwetavarshney3, 11 Jun 2012 Protein extraction, 2D-Page	0 replies 235 views	shwetavarshney3 11 Jun 2012
Help needed in radioactive calculation Started by jaya2020, 10 Jun 2012	0 replies 191 views	jaya2020 10 Jun 2012
why am I getting high km value for a enzyme Started by jaya2020, 10 Jun 2012	0 replies 178 views	jaya2020 10 Jun 2012
Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do Started by Luria Bertani, 06 Jun 2012	3 replies 266 views	Luria Bertani 10 Jun 2012
Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification Started by sonjalin, 06 Jun 2012	4 replies 310 views	Papaver 07 Jun 2012
need help with gst-tagged protein-purification Started by yourGrandma, 06 Jun 2012 protein, purification, elution	1 reply 390 views	xabiarias 06 Jun 2012