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Protein Expression and Purification

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Topic	Stats	Last Post Info
Reduce protein concentration without reduced enzyme activity Started by carollee, 04 Nov 2015 enzyme activity and 1 more...	3 replies 945 views	mdfenko 04 Nov 2015
consistent expression of overlarge proteins Started by ocram, 02 Nov 2015 bacterial expression	1 reply 645 views	labtastic 02 Nov 2015
Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? Started by 5280, 28 Oct 2015 Nuclear, Cytoplasmic and 3 more...	2 replies 1,443 views	$Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - last post by 5280$ 5280 29 Oct 2015
Is AS pp + HIC phisically possible for all proteins? Started by paramyosin, 23 Oct 2015	2 replies 776 views	labtastic 26 Oct 2015
Answered storage conditions for purified protein Started by Meg P. Anula, 19 Oct 2015 stored, temperature and 1 more...	2 replies 1,194 views	phage434 19 Oct 2015
Need to include protease inhibitor in IP wash buffer? Started by 5280, 19 Sep 2015 Immunoprecipitation and 2 more...	1 reply 968 views	gvbdxz 21 Sep 2015
Cloned gene in pET system not being expressed Started by marco.aso, 24 Aug 2015	10 replies 999 views	labtastic 14 Sep 2015
Heat shock vs eletroporation Started by Kyle Strickland, 06 Sep 2015	6 replies 1,050 views	labtastic 14 Sep 2015
lysate protein concentration Started by Raygoza, 06 Sep 2015	1 reply 646 views	labtastic 06 Sep 2015
Bradford positive but no Protein on SDS PAGE Started by VictoriaTrinkaus, 21 Jun 2015 Bradford SDS-PAGE	4 replies 927 views	Kyle Strickland 06 Sep 2015
PICHIA PASTORIS Started by Chomolungma, 24 Aug 2015	0 replies 577 views	Chomolungma 24 Aug 2015
Answered recombinant protein expression- surface or internal? Started by Meg P. Anula, 17 Aug 2015 surface, internal, protein and 2 more...	3 replies 3,091 views	pito 18 Aug 2015
Loss of protein expression by transformed bacteria Started by Jas Grewal, 17 May 2015	4 replies 1,047 views	CandyTon 10 Aug 2015
Protein localisation - IHC Started by bindyb, 23 Jul 2015 Protein, localisation and 2 more...	1 reply 3,024 views	gvbdxz 24 Jul 2015
recombinant protein vs native protein Started by KhaleesiDany, 11 Apr 2015 recombinant, native, proteins	1 reply 1,122 views	james21 21 Jul 2015
Collagen Tipe I filtration Started by Christian Ivan Velazquez G, 09 Jul 2015 COLLAGEN FILTRATION	1 reply 728 views	mdfenko 10 Jul 2015
Removal of cleaved peptide after Protease cleavage Started by marzieh, 22 Jun 2015	4 replies 822 views	mdfenko 23 Jun 2015
Disadvantage of crossflow filtration Started by trungnghiatn, 16 Jun 2015	3 replies 864 views	mdfenko 18 Jun 2015
Lactobacillus vs. lactococcus protein expression Started by Meg P. Anula, 04 Jun 2015 lactobacillus, lactococcus and 2 more...	0 replies 855 views	Meg P. Anula 04 Jun 2015
Help with Superdex200 increase Column Started by Penz871, 30 May 2015 FPLC, Gel Filtration, Superdex200	1 reply 1,607 views	labtastic 31 May 2015
Thrombin cleaved GST-fusion protein- multiple bands Started by keyspan, 21 May 2015 GST fusion protein	2 replies 868 views	ibmbio 23 May 2015
after protein purification Started by marzieh, 04 May 2015	4 replies 962 views	phage434 07 May 2015
Cloning several metabolic enzymes to screen for inhibition, how long? Started by sialic acid, 07 May 2015	1 reply 558 views	bob1 07 May 2015
Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? Started by 5280, 26 Apr 2015 Nuclear extraction and 3 more...	1 reply 622 views	5280 28 Apr 2015
Tryple express vs scraping Started by Peniel, 27 Apr 2015 protein expresssion	2 replies 628 views	Peniel 28 Apr 2015