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Protein Expression and Purification

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Topic	Stats	Last Post Info
GPCR expression in HEK293 Started by rmbio, 19 Jul 2013 Unable to get :(	2 replies 297 views	rmbio 05 Aug 2013
Histone Extraction Started by Flavia, 24 Jul 2013	0 replies 179 views	Flavia 24 Jul 2013
Protein purification from greenish supernatant of Pichia pastoris Started by recuperomn, 28 Jun 2013 Pichia pastoris greenish	10 replies 419 views	labtastic 18 Jul 2013
screening Protein Expression Started by ahmadfathi, 17 Jul 2013 Protein expression	1 reply 230 views	labtastic 18 Jul 2013
PcoldII vectors Started by sophia613, 17 Jul 2013	0 replies 198 views	sophia613 17 Jul 2013
GST tag fusion protein--- help please Started by sophia613, 14 Jul 2013 GST activity	4 replies 286 views	sophia613 17 Jul 2013
Polyclonal Ab purification Started by tkf, 15 Jul 2013	1 reply 204 views	Curtis 16 Jul 2013
purify protein from native gel Started by Kaire76, 13 Jul 2013	1 reply 197 views	mdfenko 15 Jul 2013
Recombinant expression of cytokines in mammalian cells without the propeptide re Started by guyava, 10 Jul 2013 propeptide, cytokines and 2 more...	0 replies 222 views	guyava 10 Jul 2013
chromatography question Started by Dawgology, 02 Jul 2013	2 replies 268 views	labtastic 07 Jul 2013
Is CMV enhancer essential for protein expression from CMV promoter? Started by Rollerah, 27 Jun 2013	0 replies 239 views	Rollerah 27 Jun 2013
Streptavidin purification in the lab Started by FreddieP, 19 Jun 2013	4 replies 266 views	FreddieP 24 Jun 2013
adding Tween80 to bacterial culture for protein purification ?! Started by M_shabani_medbiotech, 10 Jun 2013	3 replies 397 views	labtastic 19 Jun 2013
Membrane protein Started by manavid, 17 Jun 2013	1 reply 269 views	labtastic 19 Jun 2013
Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors Started by razasohail28@yahoo.com, 30 May 2013 GST, High GC, Protein Expression	3 replies 311 views	labtastic 08 Jun 2013
1M IPTG left for 12-14h @ Room temp - still good? Started by Frager, 06 Jun 2013 IPTG Protein expression, IPTG and 3 more...	2 replies 269 views	labtastic 08 Jun 2013
Understanding immunoprecipitation - where do the beads go? Started by DrZeus, 07 May 2013 beads, immunoprecipitation, IP and 2 more...	2 replies 384 views	@bhijit 04 Jun 2013
pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty Started by razasohail28@yahoo.com, 02 Jun 2013 RP, Codon Plus, BL21	4 replies 438 views	razasohail28@yahoo.com 04 Jun 2013
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2 3	Hot 30 replies 2,754 views	razasohail28@yahoo.com 02 Jun 2013
tag for protein purification Started by Angrysh, 17 Apr 2013	7 replies 517 views	labtastic 22 May 2013
Is it possible to use wireless connection to control and monitor FPLC? Started by mblouis, 08 May 2013 FPLC, wireless, wifi, AKTA	3 replies 572 views	zubin 21 May 2013
Problems in expression of target protein in E. coli Started by voltrapp, 16 May 2013 protein expression, his-tag	3 replies 402 views	pDNA 19 May 2013
Desalting resin Started by biotef, 17 May 2013	1 reply 204 views	mdfenko 17 May 2013
RNA extraction by trizol Started by Roshan, 16 May 2013	3 replies 238 views	Curtis 17 May 2013
Glucose Transporter 2 (GLUT2) Started by jeff kwak, 15 May 2013	0 replies 213 views	jeff kwak 15 May 2013