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Protein Expression and Purification

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Topic	Stats	Last Post Info
Cloned gene in pET system not being expressed Started by marco.aso, 24 Aug 2015	10 replies 904 views	labtastic 14 Sep 2015
Heat shock vs eletroporation Started by Kyle Strickland, 06 Sep 2015	6 replies 917 views	labtastic 14 Sep 2015
lysate protein concentration Started by Raygoza, 06 Sep 2015	1 reply 585 views	labtastic 06 Sep 2015
Bradford positive but no Protein on SDS PAGE Started by VictoriaTrinkaus, 21 Jun 2015 Bradford SDS-PAGE	4 replies 818 views	Kyle Strickland 06 Sep 2015
PICHIA PASTORIS Started by Chomolungma, 24 Aug 2015	0 replies 537 views	Chomolungma 24 Aug 2015
Answered recombinant protein expression- surface or internal? Started by Meg P. Anula, 17 Aug 2015 surface, internal, protein and 2 more...	3 replies 2,524 views	pito 18 Aug 2015
Loss of protein expression by transformed bacteria Started by Jas Grewal, 17 May 2015	4 replies 945 views	CandyTon 10 Aug 2015
Protein localisation - IHC Started by bindyb, 23 Jul 2015 Protein, localisation and 2 more...	1 reply 2,486 views	gvbdxz 24 Jul 2015
recombinant protein vs native protein Started by KhaleesiDany, 11 Apr 2015 recombinant, native, proteins	1 reply 1,015 views	james21 21 Jul 2015
Collagen Tipe I filtration Started by Christian Ivan Velazquez G, 09 Jul 2015 COLLAGEN FILTRATION	1 reply 679 views	mdfenko 10 Jul 2015
Removal of cleaved peptide after Protease cleavage Started by marzieh, 22 Jun 2015	4 replies 751 views	mdfenko 23 Jun 2015
Disadvantage of crossflow filtration Started by trungnghiatn, 16 Jun 2015	3 replies 778 views	mdfenko 18 Jun 2015
Lactobacillus vs. lactococcus protein expression Started by Meg P. Anula, 04 Jun 2015 lactobacillus, lactococcus and 2 more...	0 replies 798 views	Meg P. Anula 04 Jun 2015
Help with Superdex200 increase Column Started by Penz871, 30 May 2015 FPLC, Gel Filtration, Superdex200	1 reply 1,379 views	labtastic 31 May 2015
Thrombin cleaved GST-fusion protein- multiple bands Started by keyspan, 21 May 2015 GST fusion protein	2 replies 774 views	ibmbio 23 May 2015
after protein purification Started by marzieh, 04 May 2015	4 replies 888 views	phage434 07 May 2015
Cloning several metabolic enzymes to screen for inhibition, how long? Started by sialic acid, 07 May 2015	1 reply 517 views	bob1 07 May 2015
Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? Started by 5280, 26 Apr 2015 Nuclear extraction and 3 more...	1 reply 584 views	5280 28 Apr 2015
Tryple express vs scraping Started by Peniel, 27 Apr 2015 protein expresssion	2 replies 581 views	Peniel 28 Apr 2015
Predicting amount maximum of sample to apply onto cation exchange chromatography Started by trungnghiatn, 21 Apr 2015	5 replies 753 views	mdfenko 24 Apr 2015
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	Hot 14 replies 1,410 views	biomart 23 Apr 2015
protein expression site Started by Samantha965, 08 Apr 2015	3 replies 797 views	Samantha965 14 Apr 2015
Beads pull down only free GST Started by Franzi, 31 Mar 2015 pGEX 6p1, GST	Hot 14 replies 821 views	Franzi 10 Apr 2015
Where to obtain strain E. coli K12 SB536 ? Started by macrox, 18 Mar 2015 expression, bacteria, perisplasm	3 replies 1,196 views	phage434 18 Mar 2015
Protein Expresion and Isolation from Lactobacillus - Insoluble protein Started by silverphoenix, 12 Mar 2015	1 reply 685 views	labtastic 14 Mar 2015