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Protein Expression and Purification

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Topic	Stats	Last Post Info
after gelfiltration need to concentrate peptide mix Started by johnuknow, 02 Mar 2016 peptide	4 replies 1,754 views	mdfenko 04 Mar 2016
Quick and simple protocol for protein purification and extraction, thoughts? Started by Grant1derlin, 26 Feb 2016 protein, extraction, purification and 2 more...	2 replies 2,545 views	labtastic 29 Feb 2016
Protein that used to express really well stopped expressing. Started by bpuq, 14 Feb 2016 Protein expression and 1 more...	2 replies 1,824 views	labtastic 15 Feb 2016
Determining Cofactor for Inactive Enzyme Started by james21, 01 Feb 2016 histag, cofactor, enyme, assay	6 replies 3,044 views	bob1 04 Feb 2016
Answered study protein expression in bacteria Started by Meg P. Anula, 01 Feb 2016 protein expression, bacteria	1 reply 1,772 views	bob1 01 Feb 2016
total protein measurement from insect body Started by Inna, 03 Jan 2016	1 reply 1,520 views	bob1 04 Jan 2016
Does over-expression protein under T7 promoter in Top10 work? Started by paulomach, 15 Dec 2015	1 reply 1,559 views	labtastic 16 Dec 2015
His-tag protein not binding to resin Started by james21, 31 Jul 2015 His-tag, purification and 1 more...	9 replies 11,996 views	Kyle Strickland 05 Dec 2015
Large-scale cell growth and protein expression Started by wswr, 03 Dec 2015	1 reply 1,603 views	phage434 04 Dec 2015
Cant seem to detect HA-tagged protein with immunofluorescence after transfection Started by KhaleesiDany, 10 Nov 2015 fitc, HA-tag	0 replies 1,850 views	KhaleesiDany 10 Nov 2015
Reduce protein concentration without reduced enzyme activity Started by carollee, 04 Nov 2015 enzyme activity and 1 more...	3 replies 2,021 views	mdfenko 04 Nov 2015
consistent expression of overlarge proteins Started by ocram, 02 Nov 2015 bacterial expression	1 reply 1,338 views	labtastic 02 Nov 2015
Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? Started by 5280, 28 Oct 2015 Nuclear, Cytoplasmic and 3 more...	2 replies 3,281 views	$Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - last post by 5280$ 5280 29 Oct 2015
Is AS pp + HIC phisically possible for all proteins? Started by paramyosin, 23 Oct 2015	2 replies 1,641 views	labtastic 26 Oct 2015
Answered storage conditions for purified protein Started by Meg P. Anula, 19 Oct 2015 stored, temperature and 1 more...	2 replies 3,352 views	phage434 19 Oct 2015
Need to include protease inhibitor in IP wash buffer? Started by 5280, 19 Sep 2015 Immunoprecipitation and 2 more...	1 reply 2,503 views	gvbdxz 21 Sep 2015
Cloned gene in pET system not being expressed Started by marco.aso, 24 Aug 2015	10 replies 1,753 views	labtastic 14 Sep 2015
Heat shock vs eletroporation Started by Kyle Strickland, 06 Sep 2015	6 replies 1,758 views	labtastic 14 Sep 2015
lysate protein concentration Started by Raygoza, 06 Sep 2015	1 reply 1,337 views	labtastic 06 Sep 2015
Bradford positive but no Protein on SDS PAGE Started by VictoriaTrinkaus, 21 Jun 2015 Bradford SDS-PAGE	4 replies 1,658 views	Kyle Strickland 06 Sep 2015
PICHIA PASTORIS Started by Chomolungma, 24 Aug 2015	0 replies 1,119 views	Chomolungma 24 Aug 2015
Answered recombinant protein expression- surface or internal? Started by Meg P. Anula, 17 Aug 2015 surface, internal, protein and 2 more...	3 replies 6,046 views	pito 18 Aug 2015
Loss of protein expression by transformed bacteria Started by Jas Grewal, 17 May 2015	4 replies 1,790 views	CandyTon 10 Aug 2015
Protein localisation - IHC Started by bindyb, 23 Jul 2015 Protein, localisation and 2 more...	1 reply 5,929 views	gvbdxz 24 Jul 2015
recombinant protein vs native protein Started by KhaleesiDany, 11 Apr 2015 recombinant, native, proteins	1 reply 1,996 views	james21 21 Jul 2015