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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? Started by 5280, 26 Apr 2015 Nuclear extraction and 3 more...	1 reply 508 views	5280 28 Apr 2015
Tryple express vs scraping Started by Peniel, 27 Apr 2015 protein expresssion	2 replies 490 views	Peniel 28 Apr 2015
Predicting amount maximum of sample to apply onto cation exchange chromatography Started by trungnghiatn, 21 Apr 2015	5 replies 564 views	mdfenko 24 Apr 2015
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	Hot 14 replies 1,192 views	biomart 23 Apr 2015
protein expression site Started by Samantha965, 08 Apr 2015	3 replies 609 views	Samantha965 14 Apr 2015
Beads pull down only free GST Started by Franzi, 31 Mar 2015 pGEX 6p1, GST	Hot 14 replies 633 views	Franzi 10 Apr 2015
Where to obtain strain E. coli K12 SB536 ? Started by macrox, 18 Mar 2015 expression, bacteria, perisplasm	3 replies 900 views	phage434 18 Mar 2015
Protein Expresion and Isolation from Lactobacillus - Insoluble protein Started by silverphoenix, 12 Mar 2015	1 reply 576 views	labtastic 14 Mar 2015
protein expression Started by biomart, 10 Mar 2015 protein expression	2 replies 615 views	mdfenko 11 Mar 2015
Anion Exchange Buffers Started by Smog187, 26 Feb 2015 Anion Exchange	2 replies 806 views	labtastic 27 Feb 2015
Difficulty eluting His-tag Zn metalloprotease Started by leighann36, 16 Feb 2015 protein purification, protein and 3 more...	1 reply 1,290 views	mdfenko 16 Feb 2015
about sds-page buffers Started by marzieh, 13 Feb 2015	1 reply 653 views	mdfenko 15 Feb 2015
pet-32 Started by marzieh, 05 Jan 2015	3 replies 591 views	marzieh 13 Feb 2015
MBP fusion protein Started by singhp9, 06 Feb 2015	0 replies 794 views	singhp9 06 Feb 2015
pGreenFire1 plasmid : lipofectamine transfection? Started by sdeg, 05 Feb 2015	0 replies 914 views	sdeg 05 Feb 2015
Total protein concentration before Elisa Started by biomart, 05 Feb 2015	0 replies 1,031 views	biomart 05 Feb 2015
Question about MBP purification Started by skepticalguy99, 12 Dec 2014	2 replies 962 views	labtastic 28 Jan 2015
How do I extract soluble protein after adding SDS-PAGE buffer? Started by Rachel23, 26 Jan 2015	1 reply 636 views	labtastic 27 Jan 2015
Elute fractions that have target protein in FPLC is unstable Started by trungnghiatn, 23 Dec 2014	8 replies 765 views	$Elute fractions that have target protein in FPLC is unstable - last post by EvilTwin$ EvilTwin 16 Jan 2015
Collagen binding protein expression E. coli His-tag Started by BL118, 12 Jan 2015 his-tag, collagen binding protei and 2 more...	1 reply 1,221 views	EvilTwin 16 Jan 2015
Best epitope for IF with a poor-expresser Started by condona, 13 Jan 2015 Hela, Epitope, IF	2 replies 536 views	CPRES 15 Jan 2015
Recombinant IgT Started by haitham20eg, 08 Jan 2015 IgT, recombinant protein	0 replies 548 views	haitham20eg 08 Jan 2015
unexpected protein bands Started by ocram, 11 Dec 2014 recombinant protein expr	2 replies 556 views	labtastic 29 Dec 2014
How can i enrich highly -ve charge biomolecules using High Q resin? Started by Somnath, 23 Dec 2014	1 reply 582 views	mdfenko 23 Dec 2014
Pichia expression- His tagged protein transported to mitochondria Started by proteinz, 24 Nov 2014	0 replies 462 views	proteinz 24 Nov 2014