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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 27,177 views	sick of labwork 13 Apr 2011
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 17,850 views	mdfenko 19 Jan 2012
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 7,755 views	swanny 02 Sep 2009
protein expression Started by chn09, 15 Sep 2009 1 2	Hot 15 replies 7,002 views	ram 05 Nov 2009
protein expression (temperature issue) Started by simlez, 25 Oct 2009 1 2	Hot 17 replies 6,697 views	Adrian K 06 Dec 2010
expression in minimal media Started by otinanai, 03 Apr 2009	Hot 14 replies 5,777 views	pDNA 08 Apr 2011
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 5,521 views	Kristina @Invitrogen Dynal 09 Sep 2009
Problems with a nonspecific interaction on a Ni-NTA column Started by jessiray189, 16 Jul 2014 nonspecific, Ni-NTA, His-tag	3 replies 5,425 views	labtastic 05 Aug 2014
Protein doesn't bind any column except Ni Started by vxmqzz, 13 Mar 2009	10 replies 5,181 views	T C 07 May 2009
How to elute this protein?! Started by madrius1, 07 May 2009	5 replies 4,973 views	klinmed 30 Jul 2009
Desalting protein Started by Anna1984, 30 Jul 2009	7 replies 4,828 views	colic101 16 Sep 2009
GST-fusion protein purification Started by Niraj, 09 Jul 2009	9 replies 4,795 views	OnceHere 16 Sep 2009
Low level of expression Started by ram, 20 Jul 2009	6 replies 4,663 views	ram 27 Jul 2009
Role of imidazole in lysis buffer Started by ram, 27 Oct 2009	4 replies 4,598 views	Vini 03 Nov 2009
pgex Started by saraarasus, 27 Jan 2009	5 replies 4,591 views	swanny 10 Mar 2009
HIS-tagged protein purification Started by sashacat, 07 Jun 2011	12 replies 4,575 views	Adrian K 09 Sep 2011
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2 3	Hot 30 replies 4,497 views	razasohail28@yahoo.com 02 Jun 2013
How do I get rid of this contaminant? Started by PhD, 17 Aug 2009	13 replies 4,452 views	Prep! 09 Jun 2010
Newbie question: how to determine protein concentration after IP? Started by dc984, 05 Aug 2009	8 replies 4,384 views	little mouse 24 Aug 2009
Low expression: What else can I do? Started by Ivanov_br, 23 Aug 2009	9 replies 4,362 views	Doc Sheldon 14 Sep 2009
Antibody-beads crosslink Started by dnafactory, 15 Jun 2009	5 replies 4,323 views	dnafactory 24 Aug 2009
His-tag protein not binding to resin Started by james21, 31 Jul 2015 His-tag, purification and 1 more...	9 replies 4,300 views	Kyle Strickland 05 Dec 2015
How to purify unstable protein? Started by alam-tus, 30 Dec 2011	10 replies 4,188 views	mdfenko 05 Jan 2012
Who know anything about dialysis on protein extractions ? Started by Snoopy, 15 Mar 2009	7 replies 4,138 views	sudhakar mutyala 24 Apr 2009
Elimination of the polyethylenimine Started by paramyosin, 24 Mar 2009	9 replies 4,121 views	ElenaRM 05 Sep 2013