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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 29,345 views	sick of labwork 13 Apr 2011
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 20,383 views	mdfenko 19 Jan 2012
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 7,950 views	swanny 02 Sep 2009
protein expression Started by chn09, 15 Sep 2009 1 2	Hot 15 replies 7,345 views	ram 05 Nov 2009
protein expression (temperature issue) Started by simlez, 25 Oct 2009 1 2	Hot 17 replies 7,064 views	Adrian K 06 Dec 2010
Problems with a nonspecific interaction on a Ni-NTA column Started by jessiray189, 16 Jul 2014 nonspecific, Ni-NTA, His-tag	3 replies 6,878 views	labtastic 05 Aug 2014
expression in minimal media Started by otinanai, 03 Apr 2009	Hot 14 replies 5,969 views	pDNA 08 Apr 2011
His-tag protein not binding to resin Started by james21, 31 Jul 2015 His-tag, purification and 1 more...	9 replies 5,940 views	Kyle Strickland 05 Dec 2015
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 5,711 views	Kristina @Invitrogen Dynal 09 Sep 2009
Protein doesn't bind any column except Ni Started by vxmqzz, 13 Mar 2009	10 replies 5,399 views	T C 07 May 2009
How to elute this protein?! Started by madrius1, 07 May 2009	5 replies 5,115 views	klinmed 30 Jul 2009
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2 3	Hot 30 replies 5,040 views	razasohail28@yahoo.com 02 Jun 2013
Desalting protein Started by Anna1984, 30 Jul 2009	7 replies 4,983 views	colic101 16 Sep 2009
GST-fusion protein purification Started by Niraj, 09 Jul 2009	9 replies 4,976 views	OnceHere 16 Sep 2009
Role of imidazole in lysis buffer Started by ram, 27 Oct 2009	4 replies 4,942 views	Vini 03 Nov 2009
HIS-tagged protein purification Started by sashacat, 07 Jun 2011	12 replies 4,836 views	Adrian K 09 Sep 2011
Low level of expression Started by ram, 20 Jul 2009	6 replies 4,825 views	ram 27 Jul 2009
pgex Started by saraarasus, 27 Jan 2009	5 replies 4,783 views	swanny 10 Mar 2009
How do I get rid of this contaminant? Started by PhD, 17 Aug 2009	13 replies 4,641 views	Prep! 09 Jun 2010
Low expression: What else can I do? Started by Ivanov_br, 23 Aug 2009	9 replies 4,563 views	Doc Sheldon 14 Sep 2009
Newbie question: how to determine protein concentration after IP? Started by dc984, 05 Aug 2009	8 replies 4,536 views	little mouse 24 Aug 2009
Antibody-beads crosslink Started by dnafactory, 15 Jun 2009	5 replies 4,473 views	dnafactory 24 Aug 2009
Expression in E.coli Started by ram, 24 Jun 2010 1 2	Hot 16 replies 4,430 views	bhavana 12 Sep 2010
Who know anything about dialysis on protein extractions ? Started by Snoopy, 15 Mar 2009	7 replies 4,305 views	sudhakar mutyala 24 Apr 2009
How to purify unstable protein? Started by alam-tus, 30 Dec 2011	10 replies 4,302 views	mdfenko 05 Jan 2012