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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 30,281 views	sick of labwork 13 Apr 2011
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 21,414 views	mdfenko 19 Jan 2012
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 8,093 views	swanny 02 Sep 2009
protein expression Started by chn09, 15 Sep 2009 1 2	Hot 15 replies 7,576 views	ram 05 Nov 2009
Problems with a nonspecific interaction on a Ni-NTA column Started by jessiray189, 16 Jul 2014 nonspecific, Ni-NTA, His-tag	3 replies 7,478 views	labtastic 05 Aug 2014
protein expression (temperature issue) Started by simlez, 25 Oct 2009 1 2	Hot 17 replies 7,260 views	Adrian K 06 Dec 2010
His-tag protein not binding to resin Started by james21, 31 Jul 2015 His-tag, purification and 1 more...	9 replies 6,631 views	Kyle Strickland 05 Dec 2015
expression in minimal media Started by otinanai, 03 Apr 2009	Hot 14 replies 6,065 views	pDNA 08 Apr 2011
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 5,814 views	Kristina @Invitrogen Dynal 09 Sep 2009
Protein doesn't bind any column except Ni Started by vxmqzz, 13 Mar 2009	10 replies 5,623 views	T C 07 May 2009
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2 3	Hot 30 replies 5,355 views	razasohail28@yahoo.com 02 Jun 2013
How to elute this protein?! Started by madrius1, 07 May 2009	5 replies 5,246 views	klinmed 30 Jul 2009
GST-fusion protein purification Started by Niraj, 09 Jul 2009	9 replies 5,079 views	OnceHere 16 Sep 2009
Role of imidazole in lysis buffer Started by ram, 27 Oct 2009	4 replies 5,070 views	Vini 03 Nov 2009
Desalting protein Started by Anna1984, 30 Jul 2009	7 replies 5,068 views	colic101 16 Sep 2009
HIS-tagged protein purification Started by sashacat, 07 Jun 2011	12 replies 4,974 views	Adrian K 09 Sep 2011
Low level of expression Started by ram, 20 Jul 2009	6 replies 4,928 views	ram 27 Jul 2009
pgex Started by saraarasus, 27 Jan 2009	5 replies 4,892 views	swanny 10 Mar 2009
How do I get rid of this contaminant? Started by PhD, 17 Aug 2009	13 replies 4,734 views	Prep! 09 Jun 2010
Low expression: What else can I do? Started by Ivanov_br, 23 Aug 2009	9 replies 4,687 views	Doc Sheldon 14 Sep 2009
Newbie question: how to determine protein concentration after IP? Started by dc984, 05 Aug 2009	8 replies 4,645 views	little mouse 24 Aug 2009
Expression in E.coli Started by ram, 24 Jun 2010 1 2	Hot 16 replies 4,615 views	bhavana 12 Sep 2010
Antibody-beads crosslink Started by dnafactory, 15 Jun 2009	5 replies 4,559 views	dnafactory 24 Aug 2009
Who know anything about dialysis on protein extractions ? Started by Snoopy, 15 Mar 2009	7 replies 4,419 views	sudhakar mutyala 24 Apr 2009
How to purify unstable protein? Started by alam-tus, 30 Dec 2011	10 replies 4,390 views	mdfenko 05 Jan 2012