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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 9,682 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 19,873 views	sick of labwork 13 Apr 2011
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2 3	Hot 30 replies 2,688 views	razasohail28@yahoo.com 02 Jun 2013
Is My Protein Pure or Not? Started by rpjkmust916, 11 Feb 2011 1 2	Hot 19 replies 2,388 views	rpjkmust916 23 Feb 2011
protein expression (temperature issue) Started by simlez, 25 Oct 2009 1 2	Hot 17 replies 5,486 views	Adrian K 06 Dec 2010
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 6,940 views	swanny 02 Sep 2009
Expression in E.coli Started by ram, 24 Jun 2010 1 2	Hot 16 replies 3,221 views	bhavana 12 Sep 2010
protein expression Started by chn09, 15 Sep 2009 1 2	Hot 15 replies 5,786 views	ram 05 Nov 2009
No activity of fungi protein in E.coli Started by Biogareth, 13 Oct 2011 heteroexpression 1 2	Hot 15 replies 1,855 views	Biogareth 20 Nov 2011
expression in minimal media Started by otinanai, 03 Apr 2009	Hot 14 replies 4,866 views	pDNA 08 Apr 2011
Backgrounds persistence and higher than target protein in western blot Started by Adrian K, 21 Mar 2011	Hot 14 replies 1,999 views	Adrian K 06 Oct 2011
How do I get rid of this contaminant? Started by PhD, 17 Aug 2009	13 replies 3,988 views	Prep! 09 Jun 2010
Polysorbate 80 (Tween 80) removal Started by Negar, 22 Feb 2011	13 replies 1,958 views	Negar 04 Mar 2011
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 4,731 views	Kristina @Invitrogen Dynal 09 Sep 2009
AnionExchange problem: proteins do not bind Started by mirc, 27 May 2010	12 replies 1,532 views	NHI 12 Jul 2010
Expressing human protein in E.Coli Started by alantkl, 15 Mar 2011	12 replies 1,465 views	alantkl 01 Apr 2011
HIS-tagged protein purification Started by sashacat, 07 Jun 2011	12 replies 3,454 views	Adrian K 09 Sep 2011
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	11 replies 470 views	yingquliang 01 Nov 2013
Protein precipitation by storage Started by Mariam, 13 Oct 2013 recombinant protein and 2 more...	11 replies 223 views	Mariam 16 Oct 2013
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	11 replies 232 views	Adrian K 22 Oct 2013
Protein doesn't bind any column except Ni Started by vxmqzz, 13 Mar 2009	10 replies 4,581 views	T C 07 May 2009
How to purify unstable protein? Started by alam-tus, 30 Dec 2011	10 replies 3,347 views	mdfenko 05 Jan 2012
tagged protein size Started by zogene, 30 Jan 2013	10 replies 325 views	Curtis 31 Jan 2013
Protein purification from greenish supernatant of Pichia pastoris Started by recuperomn, 28 Jun 2013 Pichia pastoris greenish	10 replies 359 views	labtastic 18 Jul 2013
Elimination of the polyethylenimine Started by paramyosin, 24 Mar 2009	9 replies 3,337 views	ElenaRM 05 Sep 2013