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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 28,402 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 34,740 views	sick of labwork 13 Apr 2011
pET14b vector with insert transformed in BL21(DE3) RIPL cells Started by Fluffy, 05 Nov 2012 1 2 3	Hot 30 replies 7,222 views	razasohail28@yahoo.com 02 Jun 2013
Is My Protein Pure or Not? Started by rpjkmust916, 11 Feb 2011 1 2	Hot 19 replies 5,050 views	rpjkmust916 23 Feb 2011
protein expression (temperature issue) Started by simlez, 25 Oct 2009 1 2	Hot 17 replies 8,287 views	Adrian K 06 Dec 2010
Couple questions about GST purification Started by p3t3r1, 06 Aug 2009 1 2	Hot 16 replies 9,003 views	swanny 02 Sep 2009
Expression in E.coli Started by ram, 24 Jun 2010 1 2	Hot 16 replies 5,660 views	bhavana 12 Sep 2010
protein expression Started by chn09, 15 Sep 2009 1 2	Hot 15 replies 8,657 views	ram 05 Nov 2009
No activity of fungi protein in E.coli Started by Biogareth, 13 Oct 2011 heteroexpression 1 2	Hot 15 replies 4,281 views	Biogareth 20 Nov 2011
expression in minimal media Started by otinanai, 03 Apr 2009	Hot 14 replies 6,647 views	pDNA 08 Apr 2011
Backgrounds persistence and higher than target protein in western blot Started by Adrian K, 21 Mar 2011	Hot 14 replies 3,236 views	Adrian K 06 Oct 2011
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	Hot 14 replies 2,597 views	Guest_biomart_* 23 Apr 2015
Beads pull down only free GST Started by Franzi, 31 Mar 2015 pGEX 6p1, GST	Hot 14 replies 1,968 views	Franzi 10 Apr 2015
How do I get rid of this contaminant? Started by PhD, 17 Aug 2009	13 replies 5,286 views	Prep! 09 Jun 2010
Polysorbate 80 (Tween 80) removal Started by Negar, 22 Feb 2011	13 replies 4,455 views	Negar 04 Mar 2011
Immunoprecipitation Started by Danny Chow, 06 Aug 2009	12 replies 6,423 views	Kristina @Invitrogen Dynal 09 Sep 2009
AnionExchange problem: proteins do not bind Started by mirc, 27 May 2010	12 replies 2,751 views	NHI 12 Jul 2010
Expressing human protein in E.Coli Started by alantkl, 15 Mar 2011	12 replies 2,995 views	alantkl 01 Apr 2011
HIS-tagged protein purification Started by sashacat, 07 Jun 2011	12 replies 5,592 views	Adrian K 09 Sep 2011
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	11 replies 3,350 views	yingquliang 01 Nov 2013
Protein precipitation by storage Started by Mariam, 13 Oct 2013 recombinant protein and 2 more...	11 replies 2,024 views	Mariam 16 Oct 2013
Protein doesn't bind any column except Ni Started by vxmqzz, 13 Mar 2009	10 replies 6,238 views	T C 07 May 2009
How to purify unstable protein? Started by alam-tus, 30 Dec 2011	10 replies 4,945 views	mdfenko 05 Jan 2012
tagged protein size Started by zogene, 30 Jan 2013	10 replies 1,719 views	Curtis 31 Jan 2013
Protein purification from greenish supernatant of Pichia pastoris Started by recuperomn, 28 Jun 2013 Pichia pastoris greenish	10 replies 2,011 views	labtastic 18 Jul 2013