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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 28,442 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 34,766 views	sick of labwork 13 Apr 2011
Signal peptides E. coli and Methionin Started by pDNA, 28 Jan 2022	0 replies 661 views	pDNA 28 Jan 2022
Resuspending protein mixture after over-centrifuging Started by agzied98, 05 Sep 2021	1 reply 506 views	bob1 05 Sep 2021
Virus vs protein purification Started by saku98 , 11 Feb 2020	2 replies 2,513 views	jamesholder 29 Nov 2020
How to eliminate inherent proteins contamination during protein purification? Started by Ravish Godse, 15 Oct 2019 protein expression and 4 more...	1 reply 2,067 views	mdfenko 18 Oct 2019
Answered A single point mutation enhanced protein expression but attenuated its function Started by Thomson, 12 Feb 2019 single point mutation and 2 more...	4 replies 2,492 views	rkay447 28 Feb 2019
Why is my His-SUMO tag being cleaved during purification? Started by robradford, 04 Feb 2019 sumo, protein purification and 2 more...	1 reply 1,141 views	OldCloner 05 Feb 2019
How do you expose the C' biotin on membrane proteins? Started by leefuller, 12 Jan 2019	0 replies 1,125 views	leefuller 12 Jan 2019
How to obtain a non-cellular protein in its natural form? Started by Curtis, 04 Dec 2018	4 replies 1,315 views	Trof 07 Dec 2018
Protein expression issue Started by Gabriel, 24 Jul 2018 protein, purification, expression and 2 more...	4 replies 2,349 views	hops proteon 11 Sep 2018
GLUT1 and GLUT4 expression in serum Started by harry348, 04 May 2018	2 replies 1,833 views	harry348 10 Jun 2018
Time period of sodiun lauryl sarcosine Started by Siniya, 11 Apr 2018	0 replies 1,560 views	Siniya 11 Apr 2018
Time period of sodiun lauryl sarcosine Started by Siniya, 11 Apr 2018	1 reply 1,353 views	Siniya 11 Apr 2018
Outer membrane Protein storage buffer Started by Siniya, 11 Apr 2018	0 replies 1,244 views	Siniya 11 Apr 2018
Outer membrane protein extraction Started by Siniya, 06 Apr 2018	5 replies 1,558 views	Siniya 11 Apr 2018
Virus vs protein purification Started by Thomson, 12 Mar 2018 virus, protein, purification	3 replies 2,323 views	bob1 27 Mar 2018
S. aureus lysis protocol for LC-MS/MS Started by firefly2280, 21 Nov 2017 protein, bacteria, lysis and 2 more...	2 replies 2,649 views	firefly2280 04 Dec 2017
How to make antibody based affinity column for protein purification Started by ionchannelbk, 21 Sep 2017 antiboy, affinity and 3 more...	4 replies 3,513 views	mdfenko 27 Sep 2017
Agarose beads IP troubles Started by Trof, 10 Aug 2017	7 replies 2,193 views	Trof 24 Aug 2017
Problem with Rosetta gami B protein expression!!!! Started by astroboy89, 14 Jul 2017	1 reply 2,613 views	labtastic 16 Aug 2017
protein purification from agarose beads using immunoprecipation kit Started by ajitk76, 16 Jan 2017 protein purification, anti-myc and 1 more...	2 replies 4,509 views	mdfenko 27 Jul 2017
GST fusion protein - two dominant bands Started by Missle, 10 May 2016 GST, Recombinant, Purification	7 replies 2,773 views	labtastic 20 Jul 2017
The not all easy task of secreting a protein in Pichia pastoris Started by paramyosin, 19 Jul 2017 heterologous expression	0 replies 2,207 views	paramyosin 19 Jul 2017
Tagged recombinant protein has different mobility? Started by An-nur Al-Irsyad, 28 Nov 2012 tagged protein	7 replies 3,702 views	paramyosin 18 Jul 2017