Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

J Biol Methods calls for papers

Journal of Biological Methods has published its first issue and is now calling for papers.

Protein Expression and Purification

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 11,737 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 21,281 views	sick of labwork 13 Apr 2011
Anion Exchange Buffers Started by Smog187, 26 Feb 2015 Anion Exchange	2 replies 309 views	labtastic 27 Feb 2015
Difficulty eluting His-tag Zn metalloprotease Started by leighann36, 16 Feb 2015 protein purification, protein and 3 more...	1 reply 166 views	mdfenko 16 Feb 2015
about sds-page buffers Started by marzieh, 13 Feb 2015	1 reply 261 views	mdfenko 15 Feb 2015
pet-32 Started by marzieh, 05 Jan 2015	3 replies 299 views	marzieh 13 Feb 2015
MBP fusion protein Started by singhp9, 06 Feb 2015	0 replies 488 views	singhp9 06 Feb 2015
pGreenFire1 plasmid : lipofectamine transfection? Started by sdeg, 05 Feb 2015	0 replies 625 views	sdeg 05 Feb 2015
Total protein concentration before Elisa Started by biomart, 05 Feb 2015	0 replies 722 views	biomart 05 Feb 2015
Question about MBP purification Started by skepticalguy99, 12 Dec 2014	2 replies 607 views	labtastic 28 Jan 2015
How do I extract soluble protein after adding SDS-PAGE buffer? Started by Rachel23, 26 Jan 2015	1 reply 308 views	labtastic 27 Jan 2015
Elute fractions that have target protein in FPLC is unstable Started by trungnghiatn, 23 Dec 2014	8 replies 433 views	$Elute fractions that have target protein in FPLC is unstable - last post by EvilTwin$ EvilTwin 16 Jan 2015
Collagen binding protein expression E. coli His-tag Started by BL118, 12 Jan 2015 his-tag, collagen binding protei and 2 more...	1 reply 853 views	EvilTwin 16 Jan 2015
Best epitope for IF with a poor-expresser Started by condona, 13 Jan 2015 Hela, Epitope, IF	2 replies 205 views	CPRES 15 Jan 2015
Recombinant IgT Started by haitham20eg, 08 Jan 2015 IgT, recombinant protein	0 replies 263 views	haitham20eg 08 Jan 2015
unexpected protein bands Started by ocram, 11 Dec 2014 recombinant protein expr	2 replies 265 views	labtastic 29 Dec 2014
How can i enrich highly -ve charge biomolecules using High Q resin? Started by Somnath, 23 Dec 2014	1 reply 307 views	mdfenko 23 Dec 2014
Pichia expression- His tagged protein transported to mitochondria Started by proteinz, 24 Nov 2014	0 replies 280 views	proteinz 24 Nov 2014
Urea effect on mice spleen cells Started by Mariam, 23 Nov 2014 urea, mice, proliferation assay and 1 more...	1 reply 285 views	mdfenko 24 Nov 2014
Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 Started by body2013, 22 Apr 2014 in CHOmacs medium and 3 more...	4 replies 524 views	Olivia Zabel 19 Nov 2014
Non-pH-sensitive fluorophore for relative protein quantification Started by edlowry, 18 Nov 2014 pH, fluorophore, FITC, albumin and 1 more...	1 reply 386 views	bob1 18 Nov 2014
EGFP expression Started by Suvash, 30 Sep 2014	1 reply 421 views	labtastic 17 Oct 2014
Sonication of cells in ultrasound bath, without probe Started by AnetteM, 16 Oct 2014 protein expression, cell lysis and 1 more...	3 replies 468 views	labtastic 17 Oct 2014
Validating Recombinant Protein Started by bdg25, 17 Oct 2014	1 reply 319 views	labtastic 17 Oct 2014
Proteinase extraction and purification Started by hodge3, 08 Oct 2014	1 reply 403 views	mdfenko 09 Oct 2014