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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 11,478 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 21,136 views	sick of labwork 13 Apr 2011
Elute fractions that have target protein in FPLC is unstable Started by trungnghiatn, 23 Dec 2014	8 replies 350 views	$Elute fractions that have target protein in FPLC is unstable - last post by EvilTwin$ EvilTwin 16 Jan 2015
Collagen binding protein expression E. coli His-tag Started by BL118, 12 Jan 2015 his-tag, collagen binding protei and 2 more...	1 reply 529 views	EvilTwin 16 Jan 2015
Best epitope for IF with a poor-expresser Started by condona, 13 Jan 2015 Hela, Epitope, IF	2 replies 147 views	CPRES 15 Jan 2015
pet-32 Started by marzieh, 05 Jan 2015	2 replies 210 views	labtastic 09 Jan 2015
Recombinant IgT Started by haitham20eg, 08 Jan 2015 IgT, recombinant protein	0 replies 208 views	haitham20eg 08 Jan 2015
unexpected protein bands Started by ocram, 11 Dec 2014 recombinant protein expr	2 replies 201 views	labtastic 29 Dec 2014
How can i enrich highly -ve charge biomolecules using High Q resin? Started by Somnath, 23 Dec 2014	1 reply 265 views	mdfenko 23 Dec 2014
Question about MBP purification Started by skepticalguy99, 12 Dec 2014	0 replies 502 views	skepticalguy99 12 Dec 2014
Pichia expression- His tagged protein transported to mitochondria Started by proteinz, 24 Nov 2014	0 replies 234 views	proteinz 24 Nov 2014
Urea effect on mice spleen cells Started by Mariam, 23 Nov 2014 urea, mice, proliferation assay and 1 more...	1 reply 225 views	mdfenko 24 Nov 2014
Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 Started by body2013, 22 Apr 2014 in CHOmacs medium and 3 more...	4 replies 485 views	Olivia Zabel 19 Nov 2014
Non-pH-sensitive fluorophore for relative protein quantification Started by edlowry, 18 Nov 2014 pH, fluorophore, FITC, albumin and 1 more...	1 reply 305 views	bob1 18 Nov 2014
EGFP expression Started by Suvash, 30 Sep 2014	1 reply 377 views	labtastic 17 Oct 2014
Sonication of cells in ultrasound bath, without probe Started by AnetteM, 16 Oct 2014 protein expression, cell lysis and 1 more...	3 replies 399 views	labtastic 17 Oct 2014
Validating Recombinant Protein Started by bdg25, 17 Oct 2014	1 reply 275 views	labtastic 17 Oct 2014
Proteinase extraction and purification Started by hodge3, 08 Oct 2014	1 reply 344 views	mdfenko 09 Oct 2014
E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question Started by Luria Bertani, 02 Oct 2014	2 replies 353 views	Luria Bertani 08 Oct 2014
Diauxic growth after IPTG induction Started by Amro, 03 Oct 2014 IPTG growth arrest	0 replies 539 views	Amro 03 Oct 2014
the same construct, two stable cell clones with different protein size Started by Martha S, 26 Sep 2014	2 replies 351 views	Martha S 30 Sep 2014
Purification of metallothioneins? Started by Antonio Sillo Rosato, 19 Sep 2014 expression, purification and 2 more...	1 reply 459 views	labtastic 19 Sep 2014
RIPA buffer for IP and western Started by jaya2020, 09 Sep 2014	1 reply 292 views	bob1 09 Sep 2014
i am trying to get rid of unexpected protein band by centricon Started by Huongnguyen.miss, 23 Aug 2014 how to use centricon??	3 replies 356 views	labtastic 26 Aug 2014
CO- IP for nuclear protein Started by jaya2020, 19 Aug 2014	4 replies 318 views	jaya2020 22 Aug 2014