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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 19,355 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 28,405 views	sick of labwork 13 Apr 2011
Time period of sodiun lauryl sarcosine Started by Siniya, 11 Apr 2018	0 replies 124 views	Siniya 11 Apr 2018
Time period of sodiun lauryl sarcosine Started by Siniya, 11 Apr 2018	1 reply 82 views	Siniya 11 Apr 2018
Outer membrane Protein storage buffer Started by Siniya, 11 Apr 2018	0 replies 88 views	Siniya 11 Apr 2018
Outer membrane protein extraction Started by Siniya, 06 Apr 2018	5 replies 169 views	Siniya 11 Apr 2018
Virus vs protein purification Started by Thomson, 12 Mar 2018 virus, protein, purification	3 replies 385 views	bob1 27 Mar 2018
S. aureus lysis protocol for LC-MS/MS Started by firefly2280, 21 Nov 2017 protein, bacteria, lysis and 2 more...	2 replies 1,031 views	firefly2280 04 Dec 2017
How to make antibody based affinity column for protein purification Started by ionchannelbk, 21 Sep 2017 antiboy, affinity and 3 more...	4 replies 768 views	mdfenko 27 Sep 2017
Agarose beads IP troubles Started by Trof, 10 Aug 2017	7 replies 746 views	Trof 24 Aug 2017
Problem with Rosetta gami B protein expression!!!! Started by astroboy89, 14 Jul 2017	1 reply 963 views	labtastic 16 Aug 2017
protein purification from agarose beads using immunoprecipation kit Started by ajitk76, 16 Jan 2017 protein purification, anti-myc and 1 more...	2 replies 1,249 views	mdfenko 27 Jul 2017
GST fusion protein - two dominant bands Started by Missle, 10 May 2016 GST, Recombinant, Purification	7 replies 1,203 views	labtastic 20 Jul 2017
The not all easy task of secreting a protein in Pichia pastoris Started by paramyosin, 19 Jul 2017 heterologous expression	0 replies 1,077 views	paramyosin 19 Jul 2017
Tagged recombinant protein has different mobility? Started by An-nur Al-Irsyad, 28 Nov 2012 tagged protein	7 replies 2,404 views	paramyosin 18 Jul 2017
bacterial surface protein extraction Started by ozgecanerdem, 09 Jun 2017 protein extraction and 2 more...	1 reply 1,138 views	mdfenko 09 Jun 2017
peptide concentation clearification Started by samita, 07 Jun 2017	1 reply 921 views	bob1 07 Jun 2017
determination of protein concentation Started by samita, 06 Jun 2017	1 reply 729 views	bob1 06 Jun 2017
Protein concentration in mM Started by samita, 05 Jun 2017	3 replies 568 views	bob1 06 Jun 2017
peptide size either in Da or kDa Started by samita, 06 Jun 2017	1 reply 760 views	bob1 06 Jun 2017
polb cloned in pET not expressed Started by Rana2017, 08 Apr 2017 Protein Expression	2 replies 975 views	labtastic 10 Apr 2017
Anti-Penta His antibody detecting extra bands Started by Mafer, 20 Sep 2016 western blot	2 replies 1,308 views	Mafer 12 Mar 2017
Fab antibody fragment purification Started by pDNA, 13 Jan 2017	2 replies 1,119 views	Missle 18 Jan 2017
Arctic Express Cpn60 Antibody Started by Missle, 19 Dec 2016 antibody, arctic express	1 reply 1,039 views	mdfenko 20 Dec 2016
AKTA FPLC - UV instability Started by Irina1, 23 Nov 2016 FPLC	4 replies 1,071 views	mdfenko 01 Dec 2016