Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Moderators wanted

BioForum needs more volunteer moderators to provide expert help to other members by participating in forum discussions and answering questions. If you are interested, please follow this link to apply.

Protein Expression and Purification

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 9,910 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 20,114 views	sick of labwork 13 Apr 2011
Just part of the CDS (like transmembrane domain) or the full-length CDS for prot Started by Veevaa, 14 Jan 2014 fusion protein, FRET, drug screen and 1 more...	3 replies 180 views	dimensionsbio 25 Feb 2014
purified protein binds cell surface. how to be sure it is not an artifact? Started by pkay, 31 Jan 2014	1 reply 246 views	dimensionsbio 23 Feb 2014
TNES buffer and lysis buffer Started by misyz, 19 Feb 2014 Protein and proteomix	0 replies 73 views	misyz 19 Feb 2014
Pulldown of polyubiquitinated proteins Started by Wickerl3732, 15 Feb 2014	1 reply 98 views	tomnicholson 18 Feb 2014
membrane protein expression Started by ahmadfathi, 09 Feb 2014 expression	2 replies 137 views	labtastic 17 Feb 2014
Sephacryl S-300 didn't enhance the purity of my enzyme Started by Biogareth, 21 Jan 2014 purification	3 replies 135 views	dimensionsbio 29 Jan 2014
Protein secretion wtih pFuse expression system in 293T cells Started by ckarthik86, 13 Jan 2014 pFuse, 293T	2 replies 193 views	Osibisa 18 Jan 2014
Will 5mM DTT break existing disulfide bonds? Started by Missle, 16 Jan 2014 periplasm, disulfide bonds and 1 more...	3 replies 184 views	Osibisa 18 Jan 2014
his-tag purification Started by ahmadfathi, 08 Jan 2014 protein purification	9 replies 310 views	Missle 15 Jan 2014
Rate of protein production with pcDNA 3.3 transfected into HEK293 Started by Micro, 11 Nov 2013	3 replies 259 views	Osibisa 14 Jan 2014
protein extraction from cell nucleus Started by Roshan, 09 Jan 2014	2 replies 129 views	memari 09 Jan 2014
selection of the media for ion exchange chromatography Started by chn09, 31 Dec 2013 ion exchange column	1 reply 161 views	mdfenko 31 Dec 2013
Designing rPlasmid for Expression in E.Coli: Questions From A Newbie Started by djvan, 19 Dec 2013	1 reply 226 views	Missle 20 Dec 2013
Buffer to dilute and extract tobacco-leaf-trichome excreted proteins Started by Prakashbabu, 25 Nov 2013 tobacco, leaf excretion protein	0 replies 354 views	Prakashbabu 25 Nov 2013
Lysis buffer for leptin receptor and pSTAT detection Started by Maria Boije, 25 Nov 2013	1 reply 203 views	bob1 25 Nov 2013
Does a C-terminal Proline Residue Increase Degradation Risk? Started by Missle, 20 Nov 2013 Degradation, proline, recombinant	2 replies 298 views	Missle 21 Nov 2013
His-tag and GST-tag Started by weilun, 15 Nov 2013	1 reply 251 views	Missle 15 Nov 2013
Problems with biotynylated protein binding to monomeric avidin Started by Stive, 06 Oct 2013 Protein, biotin, N-Avitag	1 reply 358 views	dimensionsbio 04 Nov 2013
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	11 replies 575 views	yingquliang 01 Nov 2013
Do protein purification under partially denatured condition Started by Hellen, 23 Oct 2013 protein purification and 1 more...	1 reply 279 views	Adrian K 24 Oct 2013
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	11 replies 331 views	Adrian K 22 Oct 2013
Discoloration of Nickel affinity resins during 6-his protein purification Started by mjmspano, 21 Oct 2013 Nickel affinity, 6-his and 2 more...	1 reply 402 views	Hellen 22 Oct 2013
Phosphorylation of recombinant proteins in E. coli Started by Missle, 26 Aug 2013 recombinant, phosphorylation and 1 more...	1 reply 379 views	neuron 21 Oct 2013