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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 9,672 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 19,864 views	sick of labwork 13 Apr 2011
Buffer to dilute and extract tobacco-leaf-trichome excreted proteins Started by Prakashbabu, 25 Nov 2013 tobacco, leaf excretion protein	0 replies 100 views	Prakashbabu 25 Nov 2013
Lysis buffer for leptin receptor and pSTAT detection Started by Maria Boije, 25 Nov 2013	1 reply 70 views	bob1 25 Nov 2013
Does a C-terminal Proline Residue Increase Degradation Risk? Started by Missle, 20 Nov 2013 Degradation, proline, recombinant	2 replies 96 views	Missle 21 Nov 2013
His-tag and GST-tag Started by weilun, 15 Nov 2013	1 reply 144 views	Missle 15 Nov 2013
Rate of protein production with pcDNA 3.3 transfected into HEK293 Started by Micro, 11 Nov 2013	2 replies 134 views	Micro 12 Nov 2013
Problems with biotynylated protein binding to monomeric avidin Started by Stive, 06 Oct 2013 Protein, biotin, N-Avitag	1 reply 194 views	dimensionsbio 04 Nov 2013
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	11 replies 468 views	yingquliang 01 Nov 2013
Do protein purification under partially denatured condition Started by Hellen, 23 Oct 2013 protein purification and 1 more...	1 reply 178 views	Adrian K 24 Oct 2013
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	11 replies 231 views	Adrian K 22 Oct 2013
Discoloration of Nickel affinity resins during 6-his protein purification Started by mjmspano, 21 Oct 2013 Nickel affinity, 6-his and 2 more...	1 reply 182 views	Hellen 22 Oct 2013
Phosphorylation of recombinant proteins in E. coli Started by Missle, 26 Aug 2013 recombinant, phosphorylation and 1 more...	1 reply 290 views	neuron 21 Oct 2013
Agarose bead size in affinity chromatography Started by map3k, 14 Oct 2013 Agarose, bead, size, affinity and 1 more...	2 replies 188 views	map3k 20 Oct 2013
designing fusion protein with Flag tag Started by KristinaZ, 10 Oct 2013 Flag tag, linker and 2 more...	1 reply 261 views	dimensionsbio 18 Oct 2013
Protein precipitation by storage Started by Mariam, 13 Oct 2013 recombinant protein and 2 more...	11 replies 219 views	Mariam 16 Oct 2013
Control protein for eukaryotic expression Started by Rafmac, 04 Oct 2013	5 replies 215 views	jerryshelly1 11 Oct 2013
Flag fusion protein not detected by Flag antibody Started by KristinaZ, 01 Oct 2013 retroviral vector and 1 more...	8 replies 218 views	KristinaZ 10 Oct 2013
Does acidic Ph affect acetone precipitation? Started by Gantas, 29 Sep 2013 acetone precipitation, ph	0 replies 165 views	Gantas 29 Sep 2013
instability index of expressed protein Started by sophia613, 15 Sep 2013 Instability index: and 1 more...	1 reply 196 views	dimensionsbio 27 Sep 2013
Protein Purification-catastrophe while purifying? Started by joeysmi, 12 Sep 2013	2 replies 226 views	labtastic 25 Sep 2013
E. coli culture Started by Cristinaaaa, 20 Sep 2013	1 reply 189 views	Missle 20 Sep 2013
gfp with hsp promoter Started by MikeAdams, 20 Sep 2013	0 replies 154 views	MikeAdams 20 Sep 2013
T5 Promoter Started by bwschn01, 06 Sep 2013	1 reply 216 views	Missle 10 Sep 2013
Antibody production in Expi293 cells Started by tom43, 02 Sep 2013	2 replies 217 views	tom43 06 Sep 2013