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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 17,884 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 27,197 views	sick of labwork 13 Apr 2011
How to make antibody based affinity column for protein purification Started by ionchannelbk, 21 Sep 2017 antiboy, affinity and 3 more...	2 replies 59 views	mdfenko Yesterday, 02:24 PM
Agarose beads IP troubles Started by Trof, 10 Aug 2017	7 replies 445 views	Trof 24 Aug 2017
Problem with Rosetta gami B protein expression!!!! Started by astroboy89, 14 Jul 2017	1 reply 648 views	labtastic 16 Aug 2017
protein purification from agarose beads using immunoprecipation kit Started by ajitk76, 16 Jan 2017 protein purification, anti-myc and 1 more...	2 replies 803 views	mdfenko 27 Jul 2017
GST fusion protein - two dominant bands Started by Missle, 10 May 2016 GST, Recombinant, Purification	7 replies 968 views	labtastic 20 Jul 2017
The not all easy task of secreting a protein in Pichia pastoris Started by paramyosin, 19 Jul 2017 heterologous expression	0 replies 904 views	paramyosin 19 Jul 2017
Tagged recombinant protein has different mobility? Started by An-nur Al-Irsyad, 28 Nov 2012 tagged protein	7 replies 2,159 views	paramyosin 18 Jul 2017
bacterial surface protein extraction Started by ozgecanerdem, 09 Jun 2017 protein extraction and 2 more...	1 reply 898 views	mdfenko 09 Jun 2017
peptide concentation clearification Started by samita, 07 Jun 2017	1 reply 680 views	bob1 07 Jun 2017
determination of protein concentation Started by samita, 06 Jun 2017	1 reply 508 views	bob1 06 Jun 2017
Protein concentration in mM Started by samita, 05 Jun 2017	3 replies 341 views	bob1 06 Jun 2017
peptide size either in Da or kDa Started by samita, 06 Jun 2017	1 reply 578 views	bob1 06 Jun 2017
polb cloned in pET not expressed Started by Rana2017, 08 Apr 2017 Protein Expression	2 replies 744 views	labtastic 10 Apr 2017
Anti-Penta His antibody detecting extra bands Started by Mafer, 20 Sep 2016 western blot	2 replies 1,056 views	Mafer 12 Mar 2017
Fab antibody fragment purification Started by pDNA, 13 Jan 2017	2 replies 869 views	Missle 18 Jan 2017
Arctic Express Cpn60 Antibody Started by Missle, 19 Dec 2016 antibody, arctic express	1 reply 706 views	mdfenko 20 Dec 2016
AKTA FPLC - UV instability Started by Irina1, 23 Nov 2016 FPLC	4 replies 803 views	mdfenko 01 Dec 2016
Re-purification of His-tagged protein over IMAC Started by Missle, 21 Nov 2016 IMAC, His Tag, Purification and 1 more...	2 replies 693 views	labtastic 28 Nov 2016
Help!!-only one fragment in p3xFlag-CMV-7.1 does not work! Started by Boming Dong, 03 Nov 2016 Protein expression, plasmid	0 replies 1,655 views	Boming Dong 03 Nov 2016
Which laboratory bioreactor should I equip? Started by trungnghiatn, 26 Oct 2016 fermenter	0 replies 846 views	trungnghiatn 26 Oct 2016
Pros and Cons of refolding proteins Started by Smog187, 29 Jul 2016	3 replies 857 views	Nancy.Lee 10 Oct 2016
pKD1 based vector for K. lactis expression Started by ionchannelbk, 20 Jul 2016 pKD1, K. lactis, heterologous and 1 more...	2 replies 1,219 views	ionchannelbk 02 Aug 2016
HSP70 contamination after Nickel purification Started by Smog187, 09 Jul 2016	3 replies 810 views	labtastic 23 Jul 2016