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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 17,313 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 26,767 views	sick of labwork 13 Apr 2011
GST fusion protein - two dominant bands Started by Missle, 10 May 2016 GST, Recombinant, Purification	7 replies 830 views	labtastic 20 Jul 2017
The not all easy task of secreting a protein in Pichia pastoris Started by paramyosin, 19 Jul 2017 heterologous expression	0 replies 119 views	paramyosin 19 Jul 2017
Tagged recombinant protein has different mobility? Started by An-nur Al-Irsyad, 28 Nov 2012 tagged protein	7 replies 1,955 views	paramyosin 18 Jul 2017
Problem with Rosetta gami B protein expression!!!! Started by astroboy89, 14 Jul 2017	0 replies 213 views	astroboy89 14 Jul 2017
bacterial surface protein extraction Started by ozgecanerdem, 09 Jun 2017 protein extraction and 2 more...	1 reply 762 views	mdfenko 09 Jun 2017
peptide concentation clearification Started by samita, 07 Jun 2017	1 reply 562 views	bob1 07 Jun 2017
determination of protein concentation Started by samita, 06 Jun 2017	1 reply 381 views	bob1 06 Jun 2017
Protein concentration in mM Started by samita, 05 Jun 2017	3 replies 243 views	bob1 06 Jun 2017
peptide size either in Da or kDa Started by samita, 06 Jun 2017	1 reply 454 views	bob1 06 Jun 2017
polb cloned in pET not expressed Started by Rana2017, 08 Apr 2017 Protein Expression	2 replies 644 views	labtastic 10 Apr 2017
Anti-Penta His antibody detecting extra bands Started by Mafer, 20 Sep 2016 western blot	2 replies 900 views	Mafer 12 Mar 2017
Fab antibody fragment purification Started by pDNA, 13 Jan 2017	2 replies 726 views	Missle 18 Jan 2017
protein purification from agarose beads using immunoprecipation kit Started by ajitk76, 16 Jan 2017 protein purification, anti-myc and 1 more...	1 reply 521 views	mdfenko 16 Jan 2017
Arctic Express Cpn60 Antibody Started by Missle, 19 Dec 2016 antibody, arctic express	1 reply 575 views	mdfenko 20 Dec 2016
AKTA FPLC - UV instability Started by Irina1, 23 Nov 2016 FPLC	4 replies 682 views	mdfenko 01 Dec 2016
Re-purification of His-tagged protein over IMAC Started by Missle, 21 Nov 2016 IMAC, His Tag, Purification and 1 more...	2 replies 542 views	labtastic 28 Nov 2016
Help!!-only one fragment in p3xFlag-CMV-7.1 does not work! Started by Boming Dong, 03 Nov 2016 Protein expression, plasmid	0 replies 1,548 views	Boming Dong 03 Nov 2016
Which laboratory bioreactor should I equip? Started by trungnghiatn, 26 Oct 2016 fermenter	0 replies 689 views	trungnghiatn 26 Oct 2016
Pros and Cons of refolding proteins Started by Smog187, 29 Jul 2016	3 replies 744 views	Nancy.Lee 10 Oct 2016
pKD1 based vector for K. lactis expression Started by ionchannelbk, 20 Jul 2016 pKD1, K. lactis, heterologous and 1 more...	2 replies 1,026 views	ionchannelbk 02 Aug 2016
HSP70 contamination after Nickel purification Started by Smog187, 09 Jul 2016	3 replies 702 views	labtastic 23 Jul 2016
Answered Use of urea in solubilizing protein and its aftermath Started by Meg P. Anula, 19 Jun 2016 urea, inclusion bodies and 2 more...	5 replies 779 views	mdfenko 22 Jun 2016
Nonspecific proteins eluting with HIS-tagged peptide Started by Ahrenhase, 03 May 2016	4 replies 845 views	labtastic 30 May 2016