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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 10,033 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 20,215 views	sick of labwork 13 Apr 2011
how do you inactivate His-tag affinity beads?? Started by rockstar, 16 Apr 2014	1 reply 51 views	dimensionsbio Today, 03:30 AM
Protein Purification from mammalian cells Started by Andy1, 08 Apr 2014 Purification mammalian	1 reply 83 views	mdfenko 09 Apr 2014
DNA cleanup after linearization, before transformation? Started by Proteins-R-Us, 08 Apr 2014 Transformation, Pichia pastoris and 2 more...	1 reply 65 views	Proteins-R-Us 08 Apr 2014
Can I use cellufine sulfate cloumn as a strong cation exchange? Started by SHODAN, 02 Apr 2014	2 replies 72 views	mdfenko 03 Apr 2014
need help for transfection to synchronized cells Started by nazli, 31 Mar 2014 transfection and 2 more...	4 replies 139 views	nazli 03 Apr 2014
predict VLP formation Started by zé mann, 31 Mar 2014	1 reply 55 views	bob1 31 Mar 2014
Problems with overnights Started by Smog187, 22 Jan 2013 Overnights	5 replies 477 views	labtastic 28 Mar 2014
checking target protein in soluble and nonsoluble fraction Started by Hayat, 16 Mar 2014	0 replies 117 views	$checking target protein in soluble and nonsoluble fraction - last post by Hayat$ Hayat 16 Mar 2014
Just part of the CDS (like transmembrane domain) or the full-length CDS for prot Started by Veevaa, 14 Jan 2014 fusion protein, FRET, drug screen and 1 more...	3 replies 278 views	dimensionsbio 25 Feb 2014
purified protein binds cell surface. how to be sure it is not an artifact? Started by pkay, 31 Jan 2014	1 reply 294 views	dimensionsbio 23 Feb 2014
TNES buffer and lysis buffer Started by misyz, 19 Feb 2014 Protein and proteomix	0 replies 127 views	misyz 19 Feb 2014
Pulldown of polyubiquitinated proteins Started by Wickerl3732, 15 Feb 2014	1 reply 147 views	tomnicholson 18 Feb 2014
membrane protein expression Started by ahmadfathi, 09 Feb 2014 expression	2 replies 201 views	labtastic 17 Feb 2014
Sephacryl S-300 didn't enhance the purity of my enzyme Started by Biogareth, 21 Jan 2014 purification	3 replies 183 views	dimensionsbio 29 Jan 2014
Protein secretion wtih pFuse expression system in 293T cells Started by ckarthik86, 13 Jan 2014 pFuse, 293T	2 replies 287 views	Osibisa 18 Jan 2014
Will 5mM DTT break existing disulfide bonds? Started by Missle, 16 Jan 2014 periplasm, disulfide bonds and 1 more...	3 replies 289 views	Osibisa 18 Jan 2014
his-tag purification Started by ahmadfathi, 08 Jan 2014 protein purification	9 replies 382 views	Missle 15 Jan 2014
Rate of protein production with pcDNA 3.3 transfected into HEK293 Started by Micro, 11 Nov 2013	3 replies 294 views	Osibisa 14 Jan 2014
protein extraction from cell nucleus Started by Roshan, 09 Jan 2014	2 replies 189 views	memari 09 Jan 2014
selection of the media for ion exchange chromatography Started by chn09, 31 Dec 2013 ion exchange column	1 reply 205 views	mdfenko 31 Dec 2013
Designing rPlasmid for Expression in E.Coli: Questions From A Newbie Started by djvan, 19 Dec 2013	1 reply 276 views	Missle 20 Dec 2013
Buffer to dilute and extract tobacco-leaf-trichome excreted proteins Started by Prakashbabu, 25 Nov 2013 tobacco, leaf excretion protein	0 replies 438 views	Prakashbabu 25 Nov 2013
Lysis buffer for leptin receptor and pSTAT detection Started by Maria Boije, 25 Nov 2013	1 reply 245 views	bob1 25 Nov 2013