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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 9,916 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 20,122 views	sick of labwork 13 Apr 2011
Just part of the CDS (like transmembrane domain) or the full-length CDS for prot Started by Veevaa, 14 Jan 2014 fusion protein, FRET, drug screen and 1 more...	3 replies 189 views	dimensionsbio 25 Feb 2014
purified protein binds cell surface. how to be sure it is not an artifact? Started by pkay, 31 Jan 2014	1 reply 249 views	dimensionsbio 23 Feb 2014
TNES buffer and lysis buffer Started by misyz, 19 Feb 2014 Protein and proteomix	0 replies 77 views	misyz 19 Feb 2014
Pulldown of polyubiquitinated proteins Started by Wickerl3732, 15 Feb 2014	1 reply 101 views	tomnicholson 18 Feb 2014
membrane protein expression Started by ahmadfathi, 09 Feb 2014 expression	2 replies 141 views	labtastic 17 Feb 2014
Sephacryl S-300 didn't enhance the purity of my enzyme Started by Biogareth, 21 Jan 2014 purification	3 replies 138 views	dimensionsbio 29 Jan 2014
Protein secretion wtih pFuse expression system in 293T cells Started by ckarthik86, 13 Jan 2014 pFuse, 293T	2 replies 198 views	Osibisa 18 Jan 2014
Will 5mM DTT break existing disulfide bonds? Started by Missle, 16 Jan 2014 periplasm, disulfide bonds and 1 more...	3 replies 191 views	Osibisa 18 Jan 2014
his-tag purification Started by ahmadfathi, 08 Jan 2014 protein purification	9 replies 316 views	Missle 15 Jan 2014
Rate of protein production with pcDNA 3.3 transfected into HEK293 Started by Micro, 11 Nov 2013	3 replies 259 views	Osibisa 14 Jan 2014
protein extraction from cell nucleus Started by Roshan, 09 Jan 2014	2 replies 132 views	memari 09 Jan 2014
selection of the media for ion exchange chromatography Started by chn09, 31 Dec 2013 ion exchange column	1 reply 166 views	mdfenko 31 Dec 2013
Designing rPlasmid for Expression in E.Coli: Questions From A Newbie Started by djvan, 19 Dec 2013	1 reply 228 views	Missle 20 Dec 2013
Buffer to dilute and extract tobacco-leaf-trichome excreted proteins Started by Prakashbabu, 25 Nov 2013 tobacco, leaf excretion protein	0 replies 359 views	Prakashbabu 25 Nov 2013
Lysis buffer for leptin receptor and pSTAT detection Started by Maria Boije, 25 Nov 2013	1 reply 204 views	bob1 25 Nov 2013
Does a C-terminal Proline Residue Increase Degradation Risk? Started by Missle, 20 Nov 2013 Degradation, proline, recombinant	2 replies 307 views	Missle 21 Nov 2013
His-tag and GST-tag Started by weilun, 15 Nov 2013	1 reply 253 views	Missle 15 Nov 2013
Problems with biotynylated protein binding to monomeric avidin Started by Stive, 06 Oct 2013 Protein, biotin, N-Avitag	1 reply 367 views	dimensionsbio 04 Nov 2013
My protein doesn't bind to the Ni-NTA column Started by Qundo12, 07 Nov 2012 His-tag purification	11 replies 576 views	yingquliang 01 Nov 2013
Do protein purification under partially denatured condition Started by Hellen, 23 Oct 2013 protein purification and 1 more...	1 reply 285 views	Adrian K 24 Oct 2013
how to remove affinity tag completely Started by Hellen, 16 Oct 2013 protein purification, remove tag	11 replies 334 views	Adrian K 22 Oct 2013
Discoloration of Nickel affinity resins during 6-his protein purification Started by mjmspano, 21 Oct 2013 Nickel affinity, 6-his and 2 more...	1 reply 407 views	Hellen 22 Oct 2013
Phosphorylation of recombinant proteins in E. coli Started by Missle, 26 Aug 2013 recombinant, phosphorylation and 1 more...	1 reply 380 views	neuron 21 Oct 2013