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Protein Expression and Purification

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Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 10,399 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 20,571 views	sick of labwork 13 Apr 2011
Problems with a nonspecific interaction on a Ni-NTA column Started by jessiray189, 16 Jul 2014 nonspecific, Ni-NTA, His-tag	2 replies 234 views	jessiray189 25 Jul 2014
Trichloroacetic Acid precipitation of proteins Started by ad404, 24 Jul 2014	1 reply 152 views	mdfenko 25 Jul 2014
Missing expression of proteins of interest in Pichia pastoris Started by KatB, 30 Jan 2012 pichia pastoris, expression	1 reply 626 views	shuan 22 Jul 2014
Troubleshooting: His-Tag Nickle Column Purification Started by djvan, 16 Jun 2014	1 reply 250 views	PhDinAcronyms 08 Jul 2014
DNA cleanup after linearization, before transformation? Started by Proteins-R-Us, 08 Apr 2014 Transformation, Pichia pastoris and 2 more...	3 replies 273 views	phage434 08 Jul 2014
Answered protein expression with and without induction Started by Jaber, 05 Jul 2014	1 reply 169 views	BioMiha 06 Jul 2014
Please help me :Protein expression and purification Started by luoxuan, 25 Jun 2014	1 reply 191 views	Jaber 05 Jul 2014
Protein expression and purification - Please help Started by salema, 29 Apr 2013 protein purification and 2 more...	2 replies 470 views	CovanceABP 20 Jun 2014
genotype of e coli strain for protein expression Started by ags2014, 10 Jun 2014	2 replies 196 views	ags2014 12 Jun 2014
problem with sds-page Started by marzieh, 08 May 2014	8 replies 332 views	marzieh 09 Jun 2014
How do I go about making a set of protein standards for protein quantification v Started by dette, 02 Jun 2014	2 replies 218 views	dette 08 Jun 2014
Problems with detection of protein expressed in yeast Started by BCBT, 04 Jun 2014	1 reply 196 views	mdfenko 05 Jun 2014
thionin Started by ad404, 03 Jun 2014 thionin, protein extraction	1 reply 155 views	mdfenko 04 Jun 2014
Protein Refolding - Precipitation during dialysis Started by Luria Bertani, 26 May 2014 Refolding Protein	3 replies 200 views	mdfenko 27 May 2014
secretome and zymograms Started by _veronica_, 26 May 2014	1 reply 158 views	mdfenko 27 May 2014
Hydrophobic small-peptide conjugation using polymers Started by Puggie, 14 May 2014 PEG, Pegylation, peptide and 2 more...	0 replies 272 views	Puggie 14 May 2014
Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh Started by Epigeneticist, 11 May 2014 immunoprecipitation and 4 more...	1 reply 204 views	bob1 11 May 2014
Problem with calculations for enzyme activity Started by maja_l, 08 May 2014	2 replies 194 views	maja_l 08 May 2014
heme protein precipitation Started by Biogareth, 24 Apr 2014 heme precipitation	4 replies 227 views	labtastic 06 May 2014
regarding protein purification using 8m urea and sds page Started by atulp, 19 Apr 2014 protease	2 replies 303 views	labtastic 06 May 2014
how do you inactivate His-tag affinity beads?? Started by rockstar, 16 Apr 2014	4 replies 240 views	rockstar 27 Apr 2014
Ammonium sulfate fractionalization help Started by Andy1, 25 Apr 2014	1 reply 191 views	$Ammonium sulfate fractionalization help - last post by labtastic$ labtastic 25 Apr 2014
Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 Started by body2013, 22 Apr 2014 in CHOmacs medium and 3 more...	3 replies 238 views	bob1 23 Apr 2014