Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Moderators wanted

BioForum needs more volunteer moderators to provide expert help to other members by participating in forum discussions and answering questions. If you are interested, please follow this link to apply.

Protein Expression and Purification

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
Pinned a few questions about ion exchange chromatography Started by xaxax, 02 Nov 2011 1 2 3	Hot 31 replies 7,824 views	mdfenko 19 Jan 2012
Pinned protein expression in Pichia pastoris Started by dreamcatcher, 01 Jul 2004 1 2	Hot 26 replies 19,253 views	sick of labwork 13 Apr 2011
Is it possible to use wireless connection to control and monitor FPLC? Started by mblouis, 08 May 2013 FPLC, wireless, wifi, AKTA	3 replies 106 views	zubin Today, 05:51 PM
Problems in expression of target protein in E. coli Started by voltrapp, 16 May 2013 protein expression, his-tag	3 replies 99 views	pDNA 19 May 2013
Desalting resin Started by biotef, 17 May 2013	1 reply 51 views	mdfenko 17 May 2013
RNA extraction by trizol Started by Roshan, 16 May 2013	3 replies 56 views	Curtis 17 May 2013
Glucose Transporter 2 (GLUT2) Started by jeff kwak, 15 May 2013	0 replies 70 views	jeff kwak 15 May 2013
Contaminants in protein purification.....help! Started by Bioscientist, 02 May 2013	3 replies 130 views	labtastic 14 May 2013
tag for protein purification Started by Angrysh, 17 Apr 2013	4 replies 200 views	labtastic 14 May 2013
Membrane protein extraction Started by Roshan, 13 May 2013	2 replies 70 views	labtastic 14 May 2013
EMSA - general question Started by Drake1982, 07 May 2013 emsa	1 reply 75 views	mdfenko 08 May 2013
Understanding immunoprecipitation - where do the beads go? Started by DrZeus, 07 May 2013 beads, immunoprecipitation, IP and 2 more...	1 reply 101 views	GNANA 07 May 2013
Soluble protien of GST Started by seeking_2012, 02 May 2013 soluble GST protein	2 replies 98 views	mdfenko 06 May 2013
trouble in his-tagged protein binding with Ni-NTA agarose Started by Bioscientist, 02 May 2013	3 replies 99 views	HOYAJM 02 May 2013
Generation of recombinant yeast Started by joylex, 02 May 2013	0 replies 77 views	joylex 02 May 2013
RNA immunoprecipitation...HELP! Started by quinner, 21 Apr 2010	4 replies 1,466 views	shavetak 30 Apr 2013
Protein expression and purification - Please help Started by salema, 29 Apr 2013 protein purification and 2 more...	1 reply 129 views	Curtis 30 Apr 2013
Bacterial Media Formulation Started by Pangea, 18 Apr 2013 Media LB TB	2 replies 169 views	Pangea 24 Apr 2013
Protein purifcation without chromatography Started by 2xzwei, 23 Apr 2013	6 replies 173 views	mdfenko 23 Apr 2013
Is there posibility Reduced Glutathione cut GST-tag out of Protein Started by Hthongoc, 19 Apr 2013	0 replies 139 views	Hthongoc 19 Apr 2013
Protein is not Binding to DE52 column material Started by Monita, 10 Apr 2013 DE52, Binding of protein	2 replies 198 views	Monita 15 Apr 2013
Low expression? and reduction in OD600 in multiple strains Started by Missle, 12 Apr 2013 protein expression, host strains	0 replies 172 views	Missle 12 Apr 2013
expression problem with pBAD33...similar experience? Started by Lynn Shay, 08 Apr 2013	2 replies 176 views	Lynn Shay 11 Apr 2013
How to estimate the protein expression level from the SDS_PAGE? Started by crusoe, 01 Apr 2013 expression level, protein	8 replies 303 views	crusoe 06 Apr 2013
MBP-Tagged protein degrading after expression Started by Eniyan, 04 Apr 2013	1 reply 171 views	Missle 04 Apr 2013