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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
adjusting pH of 4x stacking buffer Started by pinkc, 14 Aug 2012	3 replies 198 views	pinkc 16 Aug 2012
Dot blot translation to WB Started by assembler01, 18 Feb 2013 dot blot, western blot, protein	2 replies 198 views	mdfenko 19 Feb 2013
2 primary antibodies, both binding actin...they shouldn't be :( Started by Ian Kockelbergh, 02 Jul 2012	3 replies 194 views	gfischer 03 Jul 2012
Reference protein give different expression Started by tierarztkarakus, 17 Feb 2013 Western blot	3 replies 194 views	bob1 18 Feb 2013
Addition of sample loading buffer, before or after heating Started by 2xzwei, 27 Sep 2012	5 replies 191 views	jmiller623 28 Sep 2012
Secondary AB blocking Started by It'sNotMe, 17 May 2012	1 reply 190 views	mdfenko 17 May 2012
SDS-PAGE : when the transfer stops Started by Tim Elliott, 03 Oct 2012	1 reply 190 views	bob1 04 Oct 2012
Alcian Blue and Silver Staining for CPS and LPS Started by Suziebee, 21 Aug 2012	3 replies 189 views	bob1 22 Aug 2012
Low-temperature SDS-PAGE: tips and suggestions Started by yulia24, 08 Jan 2013	4 replies 188 views	yulia24 08 Jan 2013
Phospho-ATF-2 coming up different sizes Started by philman, 25 Jun 2012	1 reply 186 views	doxorubicin 29 Jun 2012
Multiple bands on blot with one antibody Started by science noob, 06 Aug 2012	1 reply 184 views	Curtis 06 Aug 2012
Shift in the position of band onto the western blot Started by chimera, 16 Oct 2012	3 replies 182 views	bob1 17 Oct 2012
Using human serum as first antibody in Western/ELISA Started by ccchen777, 28 Jun 2012	3 replies 180 views	bob1 29 Jun 2012
two bands on WB-alternative use of start codons? Started by suncokret, 20 Aug 2012	2 replies 180 views	bob1 20 Aug 2012
western blot and actin issue Started by silvepa23, 10 Jan 2013	2 replies 180 views	almost a doctor 11 Jan 2013
A lot of background noise on anti-phosphotyrosine blot? Started by DrZeus, 27 Feb 2013 western blot, phospho-tyrosine and 3 more...	1 reply 180 views	GNANA 27 Feb 2013
Far western blot: probe concentration Started by Bioscientist, 11 Feb 2013	1 reply 178 views	phage434 12 Feb 2013
Tricene gel Vs. Bis-tris gel Started by immunolion, 17 Feb 2013 tricene, stacking Electrophoresis	0 replies 177 views	immunolion 17 Feb 2013
Western blot and Triton X-100 in blocking buffer Started by James., 20 Feb 2013	1 reply 177 views	Curtis 20 Feb 2013
SDS-PAGE staining for digestion assay Started by TRY, 29 May 2012	1 reply 176 views	proteaMatt 30 May 2012
Screen for luminicens detection chemiluminescence Started by helporduc, 18 May 2012	0 replies 175 views	helporduc 18 May 2012
Western blot antibody Started by weilun, 18 Apr 2013	0 replies 175 views	weilun 18 Apr 2013
Excess betamercaptoethanol during sample preparation Started by criscastells, 14 Feb 2013	4 replies 174 views	criscastells 15 Feb 2013
Trouble shooting in Far-western blot. Started by Bioscientist, 15 Mar 2013	1 reply 171 views	mdfenko 18 Mar 2013
Gradient-gel for Western Blot Started by Iwenim, 13 Aug 2012 BioRad Model 495	0 replies 169 views	Iwenim 13 Aug 2012