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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? Started by nanopore, 07 Feb 2013	5 replies 226 views	PhDinAcronyms 14 Feb 2013
Uneven beta actin Started by asta6801, 15 Apr 2013 western blot, methods	2 replies 226 views	asta6801 30 Apr 2013
turboGFP Ab for EGFP detection in WB, posible? Started by Niwrad, 24 May 2012 turboGFP antibody	1 reply 225 views	Arun Kumaran 01 Jun 2012
Low Protein Content In Cell Lysate Started by shrenik3004, 07 Feb 2013 RIPA	1 reply 225 views	Curtis 07 Feb 2013
No bands/weird bands on western blot Started by philman, 11 Mar 2013 no bands	3 replies 225 views	jerryshelly1 12 Mar 2013
Unknown silver/grey residue left after transfer. Started by molecbioguy, 10 Aug 2012	2 replies 223 views	molecbioguy 12 Aug 2012
Protein ladder not separating Started by weilun, 10 Mar 2013 SDS	6 replies 222 views	weilun 23 Mar 2013
TAU gel electrophoresis of histones Started by pakbiochemist, 01 Oct 2012	4 replies 219 views	mdfenko 05 Oct 2012
Help regarding the function of DTT for SDS-PAGE? Started by Bioscientist, 29 Aug 2012	1 reply 217 views	mdfenko 29 Aug 2012
Storage of Membrane before addition of primary antibody Started by 2xzwei, 08 Oct 2012	3 replies 216 views	cbf88 12 Oct 2012
Which antibody is good for HA-tag with low background bands? Started by memari, 18 Jan 2013 HA-tag Antibody, HA-tag	1 reply 216 views	jerryshelly1 30 Jan 2013
got band in control positive but not in sample... Started by syahida biokimia, 24 May 2012	2 replies 215 views	mhr 25 May 2012
Protein bands not migrating Started by Hakusan1, 21 Sep 2012 SDS-PAGE Silver stain	3 replies 215 views	cbf88 29 Sep 2012
question about protein expression normalization. Started by R.Mufti, 26 Sep 2012 Normalization	6 replies 215 views	cbf88 03 Oct 2012
Transferring >150 kDA proteins conditions Started by pinkc, 03 Oct 2012	3 replies 214 views	bob1 04 Oct 2012
Vertical Vs horizontal electrophoresis Started by rick112, 10 Jun 2012	3 replies 211 views	bob1 11 Jun 2012
Peculiar background problem with MPM-2 antibodies Started by mayeck, 19 Nov 2012	2 replies 211 views	mdfenko 20 Nov 2012
Problems with western blot detection Started by dude3005, 22 May 2012	1 reply 208 views	mhr 23 May 2012
protein showing up around 50 kda rather than 20 kda Started by amy in kentucky, 17 Jul 2012	2 replies 207 views	amy in kentucky 19 Jul 2012
How to prepare blood serum for western blot? Started by Tai, 19 Sep 2012	3 replies 207 views	Tai 01 Oct 2012
Detecting doublet bands in Western for eGFP control Started by bkbk, 07 Sep 2012	1 reply 203 views	bob1 08 Sep 2012
Blocking solution mistake, please help! Started by Fluffy, 15 Jan 2013	2 replies 203 views	Fluffy 16 Jan 2013
WB of cellular proteins in human serum Started by Dabronzo, 23 Jan 2013	2 replies 203 views	Dabronzo 23 Jan 2013
Multiple Non-Specific Bands in Western Blot Detected... Started by singhabhilasha.84, 13 Feb 2013 non specific bands WB	1 reply 203 views	Curtis 13 Feb 2013
Do cell signaling antibodies work with 1h RT incubation? Started by Iceage, 06 Apr 2013	2 replies 201 views	PhDinAcronyms 07 Apr 2013