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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
IgG heavy chain from IP Started by DrZeus, 05 May 2013 IP, rabbit, IgG, antibody, heavy	1 reply 174 views	bob1 05 May 2013
Native gel electrophoresis: Can it tell if a protein is forming dimer? Started by Bioscientist, 08 May 2013	1 reply 83 views	bob1 08 May 2013
Protocol for detecting soluble, secreted proteins on a Western? Started by JYaron, 13 May 2013 il-1b, western blot, caspase-1	1 reply 87 views	mdfenko 14 May 2013
Extraction of Transmembrane Proteins Started by criscastells, 21 May 2013	1 reply 52 views	GNANA 21 May 2013
Loading Controls for Serum Samples Started by Ihla, 27 Jan 2009	0 replies 1,269 views	Ihla 27 Jan 2009
Custom Antibody Started by rustyshackleford, 24 Mar 2009	0 replies 1,009 views	rustyshackleford 24 Mar 2009
Ab preabsorption Started by Nrelo, 26 Mar 2009	0 replies 1,262 views	Nrelo 26 Mar 2009
Lysyl Oxidase Started by Aris, 17 Jun 2009	0 replies 1,019 views	Aris 17 Jun 2009
His-tag antibody for western Started by kikieb, 15 Jul 2009	0 replies 1,060 views	kikieb 15 Jul 2009
Co-immunopresipitation of Nup 358/RAN BP2(nuclear pore protein) Started by amoltej, 06 Aug 2009	0 replies 1,092 views	amoltej 06 Aug 2009
FLAG Antibody conjugated with FITC Started by Teagan27, 16 Aug 2009	0 replies 1,021 views	Teagan27 16 Aug 2009
Western Blot of Bacteria Started by tylersaintD, 23 Aug 2009	0 replies 1,091 views	tylersaintD 23 Aug 2009
protein precipitation Started by plant proteomics, 17 Sep 2009	0 replies 825 views	plant proteomics 17 Sep 2009
False positive from Endogenous Antibodies? Started by jaxrich, 19 Oct 2009	0 replies 1,179 views	jaxrich 19 Oct 2009
MMP Zymography Started by jakatta70, 28 Oct 2009	0 replies 912 views	jakatta70 28 Oct 2009
Beta-actin with multiple banding Started by Sticks, 03 Dec 2009	0 replies 965 views	Sticks 03 Dec 2009
brain tissue fractionation (cytosolic and nuclear) protocol needed Started by yoshimi79, 07 Jan 2010	0 replies 1,135 views	$brain tissue fractionation (cytosolic and nuclear) protocol needed - last post by yoshimi79$ yoshimi79 07 Jan 2010
nonspecific band of c-ablin transfected cells Started by chirpy, 14 Jan 2010	0 replies 680 views	chirpy 14 Jan 2010
2x/3x SDS - different bands Started by torte, 12 Feb 2010	0 replies 710 views	torte 12 Feb 2010
Have you used SNAP id? Started by almost a doctor, 19 Feb 2010	0 replies 971 views	almost a doctor 19 Feb 2010
please help problem in zymogram Started by amaryllis, 19 Feb 2010	0 replies 747 views	amaryllis 19 Feb 2010
SDS vs RIPA for cell lysis Started by ursae, 18 Jul 2010	0 replies 896 views	ursae 18 Jul 2010
Detecting histone modifications Started by Flavia, 27 Feb 2010	0 replies 799 views	Flavia 27 Feb 2010
I have nice band from cell lysate but not from animal tissue sample...why??? Started by hazard, 10 Mar 2010	0 replies 717 views	hazard 10 Mar 2010
Loading control for Triton X-100-insoluble fraction Started by katenkak, 09 Apr 2010	0 replies 596 views	$Loading control for Triton X-100-insoluble fraction - last post by katenkak$ katenkak 09 Apr 2010