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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
The same strong bands with different primary antibody Started by Mosya, 09 Apr 2012 western blot, unspecific bands and 2 more...	1 reply 312 views	bob1 10 Apr 2012
Signal stuck on top of seperating gel Started by sarah123, 11 Apr 2012 sds-page, signal trapping	1 reply 316 views	mhr 23 May 2012
Protein extraction from dead cells Started by science noob, 18 Apr 2012	1 reply 283 views	Curtis 18 Apr 2012
What percentage gel should I make? Started by science noob, 22 Apr 2012	1 reply 285 views	mdfenko 23 Apr 2012
several questions on running SDS-PAGE Started by southpole, 25 Apr 2012	1 reply 271 views	bob1 25 Apr 2012
DMSO Started by majidatf, 26 Apr 2012 DMSO and gene expre	1 reply 227 views	bob1 26 Apr 2012
What percentage should I use for a stacking gel? Started by Genecks, 05 May 2012 percent, stacking, percentage	1 reply 403 views	proteaMatt 07 May 2012
Excess bromophenol blue in 2-D rehydration buffer Started by sudamit, 10 May 2012 2-D, Bromophenol Blue, Excess	1 reply 301 views	mdfenko 14 May 2012
western blood immunodetection Ab Started by maharith, 13 May 2012	1 reply 264 views	bob1 13 May 2012
Secondary AB blocking Started by It'sNotMe, 17 May 2012	1 reply 190 views	mdfenko 17 May 2012
Problems with western blot detection Started by dude3005, 22 May 2012	1 reply 208 views	mhr 23 May 2012
turboGFP Ab for EGFP detection in WB, posible? Started by Niwrad, 24 May 2012 turboGFP antibody	1 reply 225 views	Arun Kumaran 01 Jun 2012
SDS-PAGE staining for digestion assay Started by TRY, 29 May 2012	1 reply 176 views	proteaMatt 30 May 2012
Transfer time optimisation - Overnight low voltage vs Few hours high voltage Started by science noob, 29 May 2012	1 reply 230 views	mdfenko 30 May 2012
Use of wrong TBS concentration Started by researchgal, 01 Jun 2012 TBS, Concentration, Western Blot	1 reply 302 views	Arun Kumaran 01 Jun 2012
protein aggregation (?) in gelatin zymography Started by BradH, 04 Jun 2012 gelatin, zymography	1 reply 284 views	mdfenko 05 Jun 2012
Western Blot Antibody Epitope Started by southtowns18, 06 Jun 2012 antibody, western blot, epitope	1 reply 391 views	bob1 08 Jun 2012
Western blot narrow bands Started by kedong, 07 Jun 2012	1 reply 293 views	mdfenko 15 Jun 2012
SDS-PAGE for cell culture medium - sample preparation Started by Jaaar, 07 Jun 2012 SDS PAGE, DMEM	1 reply 372 views	proteaMatt 13 Jun 2012
A possible protocol for western blotting troubleshooting? Started by Anexum, 14 Jun 2012	1 reply 227 views	bob1 14 Jun 2012
Fold-changes different when quantifying same protein via IF and Western Started by Caribou, 19 Jun 2012 quantification, western, IF and 2 more...	1 reply 442 views	bob1 20 Jun 2012
Phospho-ATF-2 coming up different sizes Started by philman, 25 Jun 2012	1 reply 186 views	doxorubicin 29 Jun 2012
Non-specific bands and fuzzy bands Started by moerae, 17 Jul 2012	1 reply 301 views	bob1 20 Jul 2012
Reprobing a PVDF membrane which has gone dry Started by itsema, 20 Jul 2012 stripping, western blot and 2 more...	1 reply 271 views	bob1 20 Jul 2012
Multiple bands on blot with one antibody Started by science noob, 06 Aug 2012	1 reply 184 views	Curtis 06 Aug 2012