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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Strange pattern after transfer Started by Aris, 10 May 2009	8 replies 3,117 views	Aris 20 May 2009
Uneven leveling in resolving gel Started by Kamalanaadhan, 17 Sep 2009	6 replies 3,105 views	dc984 08 Oct 2009
how to deal with PVDF filter after WB Started by lufang, 04 Mar 2009	5 replies 3,073 views	yobou 13 Mar 2009
Protein Transfer Problems from SDS-PAGE Gels Started by Chiapet874, 07 Apr 2010 1 2	Hot 15 replies 3,067 views	mdfenko 19 Apr 2010
Huge background with HT22 cells phophoERK antibody Started by sikander, 12 Dec 2011 Western blotting he and 1 more...	3 replies 3,064 views	mdfenko 29 Dec 2011
Molecular weight marker Started by ram, 04 Nov 2009	13 replies 3,032 views	Feelcontraire 19 Nov 2009
sample buffer pH Started by rick112, 14 Dec 2009	12 replies 3,017 views	mdfenko 19 Feb 2010
Blue Native Gel - denaturing proteins after transfer? Started by Bobby007, 07 Aug 2009	9 replies 3,014 views	mdfenko 22 Apr 2013
Cast Bis Tris Gel with fibers in the wells Started by Aris, 26 Mar 2009	7 replies 2,993 views	mdfenko 06 Jul 2009
Using Photoshop etc on Western bot images Started by lapooh, 20 Jun 2009	8 replies 2,983 views	rajgene 01 Jul 2009
does 1% SDS prevents proteases? Started by yobou, 06 Jul 2009	8 replies 2,971 views	Tfal 17 Jul 2009
antribody gone old? Started by maris, 08 Jul 2009	11 replies 2,930 views	Tfal 17 Jul 2009
Large protein transfer problem Started by floyd78, 14 Oct 2009	8 replies 2,925 views	Feelcontraire 19 Nov 2009
Destain after PoneauS Started by Aris, 06 Aug 2009	6 replies 2,918 views	rajgene 16 Sep 2009
Protection Lanes? Started by deadally, 16 Dec 2011	4 replies 2,917 views	bob1 19 Dec 2011
Beginner Question Started by mastcells, 30 Jul 2009	6 replies 2,915 views	bob1 09 Aug 2009
Immunofluorescence and Western Blot don't match Started by OliM, 02 Apr 2009	5 replies 2,913 views	OliM 04 Apr 2009
Acrylamide/Bis Solution for Small Protein (14 kDa) Started by nirajm, 02 Sep 2009	3 replies 2,909 views	mdfenko 11 Sep 2009
changing colour of front dye in sds-page Started by kristoff, 23 Sep 2009	10 replies 2,903 views	Sheepy 14 Mar 2013
How to quantify the protein amount for serum samples ? Started by itaimo, 12 Dec 2011	1 reply 2,898 views	mdfenko 12 Dec 2011
Recombinant Protein migration Started by Ivanov_br, 27 Feb 2009	5 replies 2,880 views	Ivanov_br 20 Apr 2009
Anti-FLAG antibody help Started by charles_duh-win, 21 May 2009	3 replies 2,875 views	MaggieRoara 11 Jun 2009
SDS-PAGE of negatively charged protein Started by Inmost sun, 16 Dec 2011	4 replies 2,867 views	mdfenko 19 Dec 2011
Huge background with HT22 cells phophoERK antibody Started by sikander, 12 Dec 2011 Western blotting he	1 reply 2,865 views	Biouday 12 Dec 2011
High background.... Started by Danny Chow, 12 May 2009	6 replies 2,852 views	rajgene 01 Jul 2009