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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Problems with SDS gel Started by elysian_chow, 12 Feb 2009	6 replies 3,790 views	dcch 29 Aug 2009
Guanidinium Chloride Started by Aris, 03 Jul 2009	11 replies 3,789 views	Aris 22 Jul 2009
Problem with the SDS-PAGE sample prepaeration?!! Started by jalmali, 16 Feb 2009	6 replies 3,774 views	yobou 13 Mar 2009
Problem with running SDS page gel Started by finkish, 20 Feb 2009	6 replies 3,774 views	yobou 13 Mar 2009
freezing after reducing and boiling Started by Tfal, 20 May 2009	10 replies 3,737 views	bob1 21 Jun 2009
Markers hardly dissapeared Started by Irene G, 13 Mar 2009	10 replies 3,729 views	Nrelo 18 Mar 2009
Tie-2 westerns high background black lanes Started by alex2815, 15 Dec 2011 Tie-2 western, high background and 1 more...	13 replies 3,575 views	alex2815 20 Dec 2011
MW marker shows wrong MWs? Started by Margoute, 09 Mar 2009	8 replies 3,566 views	mikew 10 Mar 2009
Antibody Characterization Started by Neurodarcy, 07 Jul 2009	8 replies 3,554 views	Neurodarcy 01 Aug 2009
How to prepare colloidal coomassie blue? Started by WHR, 08 Jun 2009	4 replies 3,546 views	Tarum Irum 23 Nov 2009
Primary antibody incubation length Started by cancergeek, 12 Jul 2009	3 replies 3,499 views	$Primary antibody incubation length - last post by polyfractal$ polyfractal 22 Jul 2009
Ghost bands in western blot Started by pm1234, 19 Jun 2009	9 replies 3,478 views	medchemgirl 03 Dec 2009
Ponceau staining after primary and secondary? Started by mhkung, 13 May 2009	5 replies 3,444 views	Aris 14 May 2009
What's the problem with this SDS-PAGE? Started by ram, 28 Jul 2009	8 replies 3,424 views	ram 30 Jul 2009
direct cell lysate Started by SF_HK, 06 Apr 2009	8 replies 3,369 views	RNA 20 Jan 2011
Tris Glycine gel running settings Started by Aris, 27 Mar 2009	4 replies 3,314 views	Aris 27 Mar 2009
Identically loaded western bands loose intensity from left to right Started by lipidlover, 13 Dec 2011 Western, phospho-western and 3 more...	3 replies 3,309 views	EvilTwin 04 Jan 2012
BSA concentration for blocking Started by science noob, 14 Dec 2011	5 replies 3,305 views	bob1 15 Dec 2011
nitrocellulose or PVDF Started by almost a doctor, 04 Aug 2009	7 replies 3,301 views	mdfenko 12 Feb 2010
Invitrogen iBlot Started by gfischer, 18 May 2009	6 replies 3,228 views	medchemgirl 21 May 2009
SDS PAGE of Bovine Serum albumin Started by paul t, 05 May 2009	6 replies 3,214 views	paul t 06 May 2009
native PAGE? Started by yobou, 06 Feb 2009	2 replies 3,182 views	mdfenko 06 Feb 2009
bromophenol blue Started by Kami23, 28 Oct 2009	10 replies 3,169 views	mdfenko 29 Oct 2009
How does the protein fixation on SDS gel work? Started by Opal, 27 Feb 2009	3 replies 3,156 views	Opal 06 Mar 2009
Huge background with HT22 cells phophoERK antibody Started by sikander, 12 Dec 2011 Western blotting he and 1 more...	3 replies 3,135 views	mdfenko 29 Dec 2011