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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Primary antibody Started by Suhaila Taher, 27 May 2013	1 reply 82 views	Curtis 28 May 2013
Native-PAGE, help! Started by le_duy211, 24 May 2013	3 replies 134 views	mdfenko 28 May 2013
Trouble with purified recombinant protein bands Started by Bioscientist, 23 May 2013	3 replies 101 views	mdfenko 24 May 2013
Stain in electrophoresis is going from + to - Started by Gwynbleidd, 22 May 2013	3 replies 111 views	phage434 22 May 2013
Would IP make the protein of interest run slower on SDS-PAGE? Started by DrZeus, 21 May 2013 IP, SDS-PAGE, western, band, high	2 replies 147 views	mdfenko 22 May 2013
Extraction of Transmembrane Proteins Started by criscastells, 21 May 2013	1 reply 87 views	GNANA 21 May 2013
Western Blot - Quantifying Tumor samples from two different blots Started by youngscientist123456, 18 May 2013	2 replies 141 views	Iceage 20 May 2013
Protocol for detecting soluble, secreted proteins on a Western? Started by JYaron, 13 May 2013 il-1b, western blot, caspase-1	1 reply 132 views	mdfenko 14 May 2013
Protein amount to load for publication figure? Started by HOYAJM, 10 May 2013	4 replies 163 views	HOYAJM 11 May 2013
Native gel electrophoresis: Can it tell if a protein is forming dimer? Started by Bioscientist, 08 May 2013	1 reply 103 views	bob1 08 May 2013
What is different between Native protein and Denatured protein samples. Started by Tai, 08 May 2013 western blot	4 replies 199 views	mdfenko 15 May 2013
3 extra bands show up on my western after an IP Started by DrZeus, 07 May 2013 ip, igg, heavy, western, mouse	2 replies 115 views	bob1 08 May 2013
IgG heavy chain from IP Started by DrZeus, 05 May 2013 IP, rabbit, IgG, antibody, heavy	1 reply 210 views	bob1 05 May 2013
Western Blotting loading control for large proteins Started by alchemic, 30 Apr 2013	2 replies 163 views	alchemic 30 Apr 2013
Western blot antibody Started by weilun, 18 Apr 2013	0 replies 202 views	weilun 18 Apr 2013
Uneven beta actin Started by asta6801, 15 Apr 2013 western blot, methods	2 replies 257 views	asta6801 30 Apr 2013
Minimum volume antibody solution for Western blot in tray? Started by assembler01, 09 Apr 2013 antibody, volume, western blot	4 replies 278 views	bob1 09 Apr 2013
NCOR1 western Started by abrahas, 09 Apr 2013	8 replies 295 views	mdfenko 15 Apr 2013
Discrepancy between WB and Flow cytometry result Started by Tabaluga, 08 Apr 2013 Flow cytometry, Western Blot and 2 more...	5 replies 358 views	Tabaluga 08 Apr 2013
Do cell signaling antibodies work with 1h RT incubation? Started by Iceage, 06 Apr 2013	2 replies 215 views	PhDinAcronyms 07 Apr 2013
Western blotting transfer Started by Sneha Datta, 27 Mar 2013 distorted ladder bands	2 replies 380 views	Sneha Datta 29 Mar 2013
Western Quantification (No BCA???) Started by miST32, 26 Mar 2013 Western Blot and 4 more...	2 replies 251 views	jerryshelly1 28 Mar 2013
Stain nitrocellulose membrane with coomassie stain? Started by kalindi, 24 Mar 2013 nitrocellulose membrane and 1 more...	3 replies 304 views	ajithnandanam 28 Mar 2013
Western Blot: only non-specific bands and not protein of interest Started by Epigeneticist, 21 Mar 2013 Western Blot, Primary Antibody and 2 more...	3 replies 270 views	Epigeneticist 23 Mar 2013
Trouble shooting in Far-western blot. Started by Bioscientist, 15 Mar 2013	1 reply 183 views	mdfenko 18 Mar 2013