SDS-PAGE and Western Blotting
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High Molecular weight protein separationStarted by Dimilletronc, 29 Oct 2009 |
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nonreducing antibody in reducing conditionStarted by Xin, 29 Oct 2009 |
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bromophenol blueStarted by Kami23, 28 Oct 2009 |
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MMP ZymographyStarted by jakatta70, 28 Oct 2009 |
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protein stuckStarted by bsengez, 29 Sep 2009 |
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densitometric analysis for silver stained/coomassie gels?Started by alicee!, 20 Oct 2009 |
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Quantiying bands.Started by Ivanov_br, 06 Oct 2009 |
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Native Page transfer/detection problemsStarted by rly, 22 Oct 2009 |
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nuclear loading controlStarted by sllwestern, 20 Oct 2009 |
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False positive from Endogenous Antibodies?Started by jaxrich, 19 Oct 2009 |
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overloading GAPDH but nice band of interestStarted by sllwestern, 13 Oct 2009 |
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Lysis buffer for ImmunoprecipitationStarted by mtrnbh, 09 Oct 2009 |
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Uneven leveling in resolving gelStarted by Kamalanaadhan, 17 Sep 2009 |
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Could I denature the proteins that were transferred on a membrane?Started by icome, 07 Oct 2009 |
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Too many bands in my SDS-PAGEStarted by ojbillions, 17 Sep 2009 |
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Strange lane pattern, yes/no patternStarted by Aris, 02 Oct 2009 |
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no front in sds-electrophoresisStarted by lna, 05 Oct 2009 |
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Native PAGE w. Western protein markerStarted by katenkak, 01 Oct 2009 |
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Phospatase inhibitors needed for LDS Buffer?Started by zatler, 05 Mar 2009 |
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Loading control for nuclear extractsStarted by Jay Em, 28 Jan 2009 |
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anti-FLAG (M2) cross reactionStarted by PiDC, 01 Sep 2009 |
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What gel thickness and numebr of combs do you use?Started by dc984, 24 Sep 2009 |
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Can my sample be going to the buffer instead of the membrane??Started by medchemgirl, 15 Sep 2009 |
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